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Protein

Flap endonuclease 1

Gene

FEN1

Organism
Brugia malayi (Filarial nematode worm)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Magnesium 1UniRule annotation1
Binding sitei47DNA substrateUniRule annotation1
Binding sitei70DNA substrateUniRule annotation1
Metal bindingi86Magnesium 1UniRule annotation1
Metal bindingi158Magnesium 1UniRule annotation1
Binding sitei158DNA substrateUniRule annotation1
Metal bindingi160Magnesium 1UniRule annotation1
Metal bindingi179Magnesium 2UniRule annotation1
Metal bindingi181Magnesium 2UniRule annotation1
Binding sitei231DNA substrateUniRule annotation1
Metal bindingi233Magnesium 2UniRule annotation1
Binding sitei233DNA substrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:FEN1UniRule annotation
ORF Names:Bm1_49605
OrganismiBrugia malayi (Filarial nematode worm)
Taxonomic identifieri6279 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaSpiruridaSpiruromorphaFilarioideaOnchocercidaeBrugia
Proteomesi
  • UP000006672 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004035111 – 378Flap endonuclease 1Add BLAST378

Post-translational modificationi

Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA8QCH0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 104N-domainAdd BLAST104
Regioni122 – 253I-domainAdd BLAST132
Regioni336 – 344Interaction with PCNAUniRule annotation9

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

InParanoidiA8QCH0
KOiK04799
OMAiGSQDYDS
OrthoDBiEOG091G0C0E

Family and domain databases

CDDicd09867 PIN_FEN1, 1 hit
HAMAPiMF_00614 Fen, 1 hit
InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR023426 Flap_endonuc
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PANTHERiPTHR11081 PTHR11081, 1 hit
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF88723 SSF88723, 1 hit
PROSITEiView protein in PROSITE
PS00841 XPG_1, 1 hit
PS00842 XPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

A8QCH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVKDLSKVI GDHSPNSIRL KEFKGYFGRK VAVDASMCLY QFLIAVRQDG
60 70 80 90 100
SQLQTESGET TSHLLGMFYR TIRMIDNGIK PVYVFDGKPP QMKTSELEKR
110 120 130 140 150
TERRTEAEKQ RNDAVELGDE TSVNKFEKRL VKVTKEQSEE AKRLVTLMGI
160 170 180 190 200
PVLDAPCEAE AQCAALAKAG KVFATVSEDM DALTFGSPIL LRQMIASEAK
210 220 230 240 250
KLPVKEMNLN QVLKDFGMNM GQFVDLCILL GCDYVSTIRG IGPKKAFELI
260 270 280 290 300
KKYECIENVL ETINQTKYPI PQDWQYKEAR RLFLEPDVMN CENLELVWKE
310 320 330 340 350
PDVEGIVQFL CVEKSFNEDR VRGSLTRMQK GRQAAQQARI DSFFSVSKVV
360 370
TSETTKRKNE EKNNLKKRGP SLGKKAKK
Length:378
Mass (Da):42,844
Last modified:January 15, 2008 - v1
Checksum:i8E9DAD594F202030
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DS239429 Genomic DNA Translation: EDP30068.1
RefSeqiXP_001901388.1, XM_001901353.1

Genome annotation databases

EnsemblMetazoaiBm13951; Bm13951; WBGene00234212
GeneIDi6104806
KEGGibmy:Bm1_49605
WBParaSiteiBm13951; Bm13951; WBGene00234212

Similar proteinsi

Entry informationi

Entry nameiFEN1_BRUMA
AccessioniPrimary (citable) accession number: A8QCH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: January 15, 2008
Last modified: March 28, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health