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Protein

Isocitrate lyase

Gene

ACU-7

Organism
Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathway: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (ACU-7)
  2. Malate synthase (CC1G_02345)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi165 – 1651MagnesiumBy similarity
Active sitei203 – 2031Proton acceptorBy similarity
Binding sitei240 – 2401SubstrateBy similarity
Binding sitei455 – 4551SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:ACU-7By similarity
ORF Names:CC1G_07122
OrganismiCoprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata)
Taxonomic identifieri240176 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPsathyrellaceaeCoprinopsis
ProteomesiUP000001861 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:CC1G_07122.

Subcellular locationi

  • Glyoxysome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Isocitrate lyasePRO_0000333260Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi240176.XP_001835698.1.

Structurei

3D structure databases

ProteinModelPortaliA8NR45.
SMRiA8NR45. Positions 1-518.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 963Substrate bindingBy similarity
Regioni204 – 2052Substrate bindingBy similarity
Regioni420 – 4245Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi536 – 5383Microbody targeting signalSequence Analysis

Sequence similaritiesi

Phylogenomic databases

InParanoidiA8NR45.
KOiK01637.
OrthoDBiEOG73Z331.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8NR45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSERAQFAA EVAEVERWWK SPRFARVNRP YTAADVVSKR GTIKINYPSD
60 70 80 90 100
VQGKKLWKLL SEHAKNGTPS HTYGALDPVQ VTQMAKYLET VYVSGWQSSS
110 120 130 140 150
TASSSNEPGP DLADYPSNTV PNKVEHLFMA QLFHDRKQRE ARSRMSDAEL
160 170 180 190 200
ANTPVIDYLR PIVADADTGH GGLTAVMKLT KMFVEKGAAG IHIEDQAPGT
210 220 230 240 250
KKCGHMAGKV LVPIQEHINR LVAIRLQYDI MGVENLVVAR TDSEAATLIT
260 270 280 290 300
SNIDDRDHPF IQGSTNPSLP PLNNVMVEAE AQGKTGDQLQ AIEDGWIKAA
310 320 330 340 350
NLQLFPQALA QALANEGASR STVEKLVARV SRLSWPQAVA VAQKEFGLKQ
360 370 380 390 400
VPYWNWDAPR TREGYYRYQG GTECAIHRAN AFAPYADLLW METKKPILAQ
410 420 430 440 450
AKEFAAGVHA VHPGQWLAYN LSPSFNWEAA GLNAQDMQAY VWELGKLGFV
460 470 480 490 500
WQFITLAGLH SNAYISDLFA QNFAKTGMKA YVELVQSRER EIGCDVLTHQ
510 520 530
KWSGADYADS LIKTVTGGVS STAAMGAGVT ESQFTSKL
Length:538
Mass (Da):59,243
Last modified:January 15, 2008 - v1
Checksum:i75A0624B3453C0C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACS02000008 Genomic DNA. Translation: EAU86043.1.
RefSeqiXP_001835698.1. XM_001835646.1.

Genome annotation databases

GeneIDi6012236.
KEGGicci:CC1G_07122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACS02000008 Genomic DNA. Translation: EAU86043.1.
RefSeqiXP_001835698.1. XM_001835646.1.

3D structure databases

ProteinModelPortaliA8NR45.
SMRiA8NR45. Positions 1-518.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi240176.XP_001835698.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi6012236.
KEGGicci:CC1G_07122.

Organism-specific databases

EuPathDBiFungiDB:CC1G_07122.

Phylogenomic databases

InParanoidiA8NR45.
KOiK01637.
OrthoDBiEOG73Z331.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus)."
    Stajich J.E., Wilke S.K., Ahren D., Au C.H., Birren B.W., Borodovsky M., Burns C., Canbaeck B., Casselton L.A., Cheng C.K., Deng J., Dietrich F.S., Fargo D.C., Farman M.L., Gathman A.C., Goldberg J., Guigo R., Hoegger P.J.
    , Hooker J.B., Huggins A., James T.Y., Kamada T., Kilaru S., Kodira C., Kuees U., Kupfer D., Kwan H.S., Lomsadze A., Li W., Lilly W.W., Ma L.-J., Mackey A.J., Manning G., Martin F., Muraguchi H., Natvig D.O., Palmerini H., Ramesh M.A., Rehmeyer C.J., Roe B.A., Shenoy N., Stanke M., Ter-Hovhannisyan V., Tunlid A., Velagapudi R., Vision T.J., Zeng Q., Zolan M.E., Pukkila P.J.
    Proc. Natl. Acad. Sci. U.S.A. 107:11889-11894(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003.

Entry informationi

Entry nameiACEA_COPC7
AccessioniPrimary (citable) accession number: A8NR45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 15, 2008
Last modified: June 24, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.