A8NR45 (ACEA_COPC7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate lyase
Short name=ICL
Short name=Isocitrase
Short name=Isocitratase
EC=4.1.3.1

Gene names

Name:	ACU-7
ORF Names:	CC1G_07122

Organism

Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata) [Reference proteome]

Taxonomic identifier

240176 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Agaricomycetes › Agaricomycetidae › Agaricales › Psathyrellaceae › Coprinopsis › Coprinopsis cinerea

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate = succinate + glyoxylate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Peroxisome
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Isocitrate lyase		PRO_0000333260
Regions
	Motif	536 – 538	3	Microbody targeting signal Potential
Sites
	Active site	203	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8NR45 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 75A0624B3453C0C2	FASTA	538	59,243
	10 20 30 40 50 60 MSSERAQFAA EVAEVERWWK SPRFARVNRP YTAADVVSKR GTIKINYPSD VQGKKLWKLL 70 80 90 100 110 120 SEHAKNGTPS HTYGALDPVQ VTQMAKYLET VYVSGWQSSS TASSSNEPGP DLADYPSNTV 130 140 150 160 170 180 PNKVEHLFMA QLFHDRKQRE ARSRMSDAEL ANTPVIDYLR PIVADADTGH GGLTAVMKLT 190 200 210 220 230 240 KMFVEKGAAG IHIEDQAPGT KKCGHMAGKV LVPIQEHINR LVAIRLQYDI MGVENLVVAR 250 260 270 280 290 300 TDSEAATLIT SNIDDRDHPF IQGSTNPSLP PLNNVMVEAE AQGKTGDQLQ AIEDGWIKAA 310 320 330 340 350 360 NLQLFPQALA QALANEGASR STVEKLVARV SRLSWPQAVA VAQKEFGLKQ VPYWNWDAPR 370 380 390 400 410 420 TREGYYRYQG GTECAIHRAN AFAPYADLLW METKKPILAQ AKEFAAGVHA VHPGQWLAYN 430 440 450 460 470 480 LSPSFNWEAA GLNAQDMQAY VWELGKLGFV WQFITLAGLH SNAYISDLFA QNFAKTGMKA 490 500 510 520 530 YVELVQSRER EIGCDVLTHQ KWSGADYADS LIKTVTGGVS STAAMGAGVT ESQFTSKL

References

[1]

"Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus)." Stajich J.E., Wilke S.K., Ahren D., Au C.H., Birren B.W., Borodovsky M., Burns C., Canbaeck B., Casselton L.A., Cheng C.K., Deng J., Dietrich F.S., Fargo D.C., Farman M.L., Gathman A.C., Goldberg J., Guigo R., Hoegger P.J. , Hooker J.B., Huggins A., James T.Y., Kamada T., Kilaru S., Kodira C., Kuees U., Kupfer D., Kwan H.S., Lomsadze A., Li W., Lilly W.W., Ma L.-J., Mackey A.J., Manning G., Martin F., Muraguchi H., Natvig D.O., Palmerini H., Ramesh M.A., Rehmeyer C.J., Roe B.A., Shenoy N., Stanke M., Ter-Hovhannisyan V., Tunlid A., Velagapudi R., Vision T.J., Zeng Q., Zolan M.E., Pukkila P.J. Proc. Natl. Acad. Sci. U.S.A. 107:11889-11894(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACS02000008 Genomic DNA. Translation: EAU86043.1.
RefSeq	XP_001835698.1. XM_001835646.1.
3D structure databases
ProteinModelPortal	A8NR45.
SMR	A8NR45. Positions 1-518.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6012236.
KEGG	cci:CC1G_07122.
Phylogenomic databases
KO	K01637.
OrthoDB	EOG4P5PJP.
Enzyme and pathway databases
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 2 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_COPC7

Accession

Primary (citable) accession number: A8NR45

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 15, 2008
Last modified:	March 6, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program