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A8NFF0 (TRMB_BRUMA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
ORF Names:Bm1_01445
OrganismBrugia malayi (Filarial nematode worm) [Complete proteome]
Taxonomic identifier6279 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaSpiruridaFilarioideaOnchocercidaeBrugia

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 258258tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370562

Regions

Region99 – 1002S-adenosyl-L-methionine binding By similarity
Region132 – 1332S-adenosyl-L-methionine binding By similarity
Region230 – 2323S-adenosyl-L-methionine binding By similarity

Sites

Active site1551 By similarity
Binding site761S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1521S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A8NFF0 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 545EF608E4B807F2

FASTA25830,286
        10         20         30         40         50         60 
MVSTENKIGL FKNKDDDIDG EEMRELPQKK FYRQRAHANP ISDHEFDYPV FPEQMDWKKY 

        70         80         90        100        110        120 
FGDFSEGRQV EFADVGCGYG GLLIKLSTLY PEALMVGLEI RVKVSDYVQD KIHALRLREP 

       130        140        150        160        170        180 
GNYRNVACLR TNAMKYLPNY FRRHQLTKMF FLYPDPHFKK AKHKWRIITP TLLAEYAYVL 

       190        200        210        220        230        240 
KPGGLVYTIT DVEELHIWMV RHLSAHPLFE RLTDLEMKMD PVVEMLYDST EEGQKVARNE 

       250 
GSKWSAVFRR LPNPVLSS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DS237063 Genomic DNA. Translation: EDP39397.1.
RefSeqXP_001891805.1. XM_001891770.1.

3D structure databases

ProteinModelPortalA8NFF0.
SMRA8NFF0. Positions 48-256.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6095251.
KEGGbmy:Bm1_01445.

Organism-specific databases

CTD6095251.

Phylogenomic databases

InParanoidA8NFF0.
KOK03439.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BRUMA
AccessionPrimary (citable) accession number: A8NFF0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 15, 2008
Last modified: June 11, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways