A8NFF0 (TRMB_BRUMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

ORF Names:

Bm1_01445

Organism

Brugia malayi (Filarial nematode worm) [Complete proteome]

Taxonomic identifier

6279 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Spirurida › Filarioidea › Onchocercidae › Brugia

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370562
Regions
	Region	99 – 100	2	S-adenosyl-L-methionine binding By similarity
	Region	132 – 133	2	S-adenosyl-L-methionine binding By similarity
	Region	230 – 232	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	155	1	By similarity
	Binding site	76	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	152	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8NFF0 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 545EF608E4B807F2	FASTA	258	30,286
	10 20 30 40 50 60 MVSTENKIGL FKNKDDDIDG EEMRELPQKK FYRQRAHANP ISDHEFDYPV FPEQMDWKKY 70 80 90 100 110 120 FGDFSEGRQV EFADVGCGYG GLLIKLSTLY PEALMVGLEI RVKVSDYVQD KIHALRLREP 130 140 150 160 170 180 GNYRNVACLR TNAMKYLPNY FRRHQLTKMF FLYPDPHFKK AKHKWRIITP TLLAEYAYVL 190 200 210 220 230 240 KPGGLVYTIT DVEELHIWMV RHLSAHPLFE RLTDLEMKMD PVVEMLYDST EEGQKVARNE 250 GSKWSAVFRR LPNPVLSS

References

[1]

"Draft genome of the filarial nematode parasite Brugia malayi." Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E., Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T., Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L. , Schatz M., Shumway M., Koo H., Salzberg S.L., Schobel S., Pertea M., Pop M., White O., Barton G.J., Carlow C.K.S., Crawford M.J., Daub J., Dimmic M.W., Estes C.F., Foster J.M., Ganatra M., Gregory W.F., Johnson N.M., Jin J., Komuniecki R., Korf I., Kumar S., Laney S., Li B.-W., Li W., Lindblom T.H., Lustigman S., Ma D., Maina C.V., Martin D.M., McCarter J.P., McReynolds L., Mitreva M., Nutman T.B., Parkinson J., Peregrin-Alvarez J.M., Poole C., Ren Q., Saunders L., Sluder A.E., Smith K., Stanke M., Unnasch T.R., Ware J., Wei A.D., Weil G., Williams D.J., Zhang Y., Williams S.A., Fraser-Liggett C., Slatko B., Blaxter M.L., Scott A.L. Science 317:1756-1760(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS237063 Genomic DNA. Translation: EDP39397.1.
RefSeq	XP_001891805.1. XM_001891770.1.
3D structure databases
ProteinModelPortal	A8NFF0.
SMR	A8NFF0. Positions 48-256.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6095251.
KEGG	bmy:Bm1_01445.
Organism-specific databases
CTD	6095251.
Phylogenomic databases
InParanoid	A8NFF0.
KO	K03439.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_BRUMA

Accession

Primary (citable) accession number: A8NFF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	January 15, 2008
Last modified:	October 3, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)