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A8NFF0

- TRMB_BRUMA

UniProt

A8NFF0 - TRMB_BRUMA

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
Bm1_01445
Organism
Brugia malayi (Filarial nematode worm)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei152 – 1521S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei155 – 1551 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
ORF Names:Bm1_01445
OrganismiBrugia malayi (Filarial nematode worm)
Taxonomic identifieri6279 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaSpiruridaFilarioideaOnchocercidaeBrugia
ProteomesiUP000006672: Unassembled WGS sequence

Subcellular locationi

Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationPRO_0000370562Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA8NFF0.
SMRiA8NFF0. Positions 48-256.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 1002S-adenosyl-L-methionine binding By similarity
Regioni132 – 1332S-adenosyl-L-methionine binding By similarity
Regioni230 – 2323S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiA8NFF0.
KOiK03439.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8NFF0-1 [UniParc]FASTAAdd to Basket

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MVSTENKIGL FKNKDDDIDG EEMRELPQKK FYRQRAHANP ISDHEFDYPV    50
FPEQMDWKKY FGDFSEGRQV EFADVGCGYG GLLIKLSTLY PEALMVGLEI 100
RVKVSDYVQD KIHALRLREP GNYRNVACLR TNAMKYLPNY FRRHQLTKMF 150
FLYPDPHFKK AKHKWRIITP TLLAEYAYVL KPGGLVYTIT DVEELHIWMV 200
RHLSAHPLFE RLTDLEMKMD PVVEMLYDST EEGQKVARNE GSKWSAVFRR 250
LPNPVLSS 258
Length:258
Mass (Da):30,286
Last modified:January 15, 2008 - v1
Checksum:i545EF608E4B807F2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS237063 Genomic DNA. Translation: EDP39397.1.
RefSeqiXP_001891805.1. XM_001891770.1.

Genome annotation databases

GeneIDi6095251.
KEGGibmy:Bm1_01445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DS237063 Genomic DNA. Translation: EDP39397.1 .
RefSeqi XP_001891805.1. XM_001891770.1.

3D structure databases

ProteinModelPortali A8NFF0.
SMRi A8NFF0. Positions 48-256.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 6095251.
KEGGi bmy:Bm1_01445.

Organism-specific databases

CTDi 6095251.

Phylogenomic databases

InParanoidi A8NFF0.
KOi K03439.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Draft genome of the filarial nematode parasite Brugia malayi."
    Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E., Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T., Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L.
    , Schatz M., Shumway M., Koo H., Salzberg S.L., Schobel S., Pertea M., Pop M., White O., Barton G.J., Carlow C.K.S., Crawford M.J., Daub J., Dimmic M.W., Estes C.F., Foster J.M., Ganatra M., Gregory W.F., Johnson N.M., Jin J., Komuniecki R., Korf I., Kumar S., Laney S., Li B.-W., Li W., Lindblom T.H., Lustigman S., Ma D., Maina C.V., Martin D.M., McCarter J.P., McReynolds L., Mitreva M., Nutman T.B., Parkinson J., Peregrin-Alvarez J.M., Poole C., Ren Q., Saunders L., Sluder A.E., Smith K., Stanke M., Unnasch T.R., Ware J., Wei A.D., Weil G., Williams D.J., Zhang Y., Williams S.A., Fraser-Liggett C., Slatko B., Blaxter M.L., Scott A.L.
    Science 317:1756-1760(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiTRMB_BRUMA
AccessioniPrimary (citable) accession number: A8NFF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 15, 2008
Last modified: June 11, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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