Reviewed, UniProtKB/Swiss-Prot A8N2T3 (PMIP_COPC7)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
ORF Names:	CC1G_01835

Organism

Coprinopsis cinerea (strain Okayama-7 / 130 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata)

Taxonomic identifier

240176 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Psathyrellaceae › Coprinopsis

Protein attributes

Sequence length	777 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion Potential
	Chain	40 – 777	738	Mitochondrial intermediate peptidase		PRO_0000338580
Regions
	Compositional bias	490 – 493	4	Poly-Asp
Sites
	Active site	562	1	By similarity
	Metal binding	561	1	Zinc; catalytic By similarity
	Metal binding	565	1	Zinc; catalytic By similarity
	Metal binding	568	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8N2T3-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 1BE83120B6ECF1B2	FASTA	777	87,437
	10 20 30 40 50 60 MLSSSARTVL ARHSARQLYR FRGCLVHQQR HRHQVQRTLA THVQRHLPAS LDDKALVALF 70 80 90 100 110 120 DQPNGSKLRS PFNTTGLFGH PNLTHPRALV SLAESTLVRA QLLTQRILEA GKSEHELAKV 130 140 150 160 170 180 VKNLDRLSDM LCGVIDLAEL VRNAHPDRLW VEAANHAYET LCEFMNVLNT HTGLYEVLKQ 190 200 210 220 230 240 VLSNPTLVNS LSPEAYQTAL IFWRDFEKSA IDLPPAQRNK FVSLSSDILV LGRQFLENAS 250 260 270 280 290 300 TPRPPTSIKA SDLAGLKDKG MGVRLQLQAQ FTNRDLQIYP GSLQAHMIMR SAPNEEPRRR 310 320 330 340 350 360 LYLAANSSTQ EQIEVLEALL KKRAELAQLV GRESFAHMTL DDKMAKTPDN VTNFLDALID 370 380 390 400 410 420 HTRPFARSAL RTLAQRKQAH HGLSSLPIIQ AWDRDFYCPP DPPAPPIPLP PLTLGTVFMG 430 440 450 460 470 480 LSRLFRHLYG VSLRPVPSAS GEVWHTDVQK LEVVDEDQGI IGWIYADLFA RRGKASGAAH 490 500 510 520 530 540 YTVRCSRRTD DDDEQGDGMF EGAELQILES QEFEAVKRHR LPNQEGVFQL PLVVLLCEFT 550 560 570 580 590 600 RPTVSKGGTI LEWHEVQTLF HEMGHAMHSM LGRTEYQNVS GTRCATDFVE LPSILMEHFL 610 620 630 640 650 660 NSPAVLSLFD ADGTSTLRQI GNHHHDPCHA IDTYSQIMLA VVDQIYHSPT VLDPSFDSTR 670 680 690 700 710 720 EYGNLQNTRG LIPYVPGTSY QTQFGHLFGY GATYYSYLFD RAIASRVWSK VFSKDPLDRE 730 740 750 760 770 LGEKYKREVL RWGGARDPWE MVATLLDAPE LAAGDAEAMR EVGRWRIEDE VGVGGRH

References

[1]

"Annotation of the Coprinopsis cinerea genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K., Pushparaj V. , DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Kodira C.D., Zeng Q., Alvarado L., Yandava C., Oleary S., Dietrich F.S., Pukkila P.J. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACS01000026 Genomic DNA. Translation: EAU92790.1.
RefSeq	XP_001829155.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	6005581.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_COPC7

Accession

Primary (citable) accession number: A8N2T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	January 15, 2008
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)