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A8N1T1

- LIPA_COPC7

UniProt

A8N1T1 - LIPA_COPC7

Protein

Lipoyl synthase, mitochondrial

Gene

CC1G_03624

Organism
Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi110 – 1101Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi144 – 1441Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    ORF Names:CC1G_03624
    OrganismiCoprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) (Inky cap fungus) (Hormographiella aspergillata)
    Taxonomic identifieri240176 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPsathyrellaceaeCoprinopsis
    ProteomesiUP000001861: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1818MitochondrionUniRule annotationAdd
    BLAST
    Chaini19 – 390372Lipoyl synthase, mitochondrialPRO_0000398264Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliA8N1T1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03644.
    OrthoDBiEOG79KPR7.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A8N1T1-1 [UniParc]FASTAAdd to Basket

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    MALYRAPKLQ RSLLNRCLAT ASTTPAAAST SRTSTFRKTL EEGPTLDDFI    50
    AGDVPNNRVV LGNTSAPRLP SYLKTSIPTG ASFSKIKKDL RGLNLHTVCE 100
    EARCPNIGDC WGGKPGATEA EGRSAATATI MLMGDTCTRG CRFCSVKTSR 150
    TPPPLDPHEP ENTAEAISRW GLGYIVLTSV DRDDLADGGA RHFAETISKI 200
    KQKAPHILVE ALTGDFAGNL EHVSLVAKSG LDVYAHNIET VEALTPFVRD 250
    RRATFRQSLS VLKRAKEEGV KVTKTSIMLG VGETEDQVLD ALKELRKVDV 300
    DVVTFGQYMR PTKRHMKVDR YVEPAEFDRW KQVAEDLGFL YVASGPLVRS 350
    SYKAGEFYIE NVLKGKSVNK RVKNLTMESE LSEKSASASL 390
    Length:390
    Mass (Da):42,711
    Last modified:January 15, 2008 - v1
    Checksum:i2D13896927A3B057
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACS02000001 Genomic DNA. Translation: EAU92837.1.
    RefSeqiXP_001828830.1. XM_001828778.2.

    Genome annotation databases

    GeneIDi6005256.
    KEGGicci:CC1G_03624.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACS02000001 Genomic DNA. Translation: EAU92837.1 .
    RefSeqi XP_001828830.1. XM_001828778.2.

    3D structure databases

    ProteinModelPortali A8N1T1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6005256.
    KEGGi cci:CC1G_03624.

    Phylogenomic databases

    KOi K03644.
    OrthoDBi EOG79KPR7.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus)."
      Stajich J.E., Wilke S.K., Ahren D., Au C.H., Birren B.W., Borodovsky M., Burns C., Canbaeck B., Casselton L.A., Cheng C.K., Deng J., Dietrich F.S., Fargo D.C., Farman M.L., Gathman A.C., Goldberg J., Guigo R., Hoegger P.J.
      , Hooker J.B., Huggins A., James T.Y., Kamada T., Kilaru S., Kodira C., Kuees U., Kupfer D., Kwan H.S., Lomsadze A., Li W., Lilly W.W., Ma L.-J., Mackey A.J., Manning G., Martin F., Muraguchi H., Natvig D.O., Palmerini H., Ramesh M.A., Rehmeyer C.J., Roe B.A., Shenoy N., Stanke M., Ter-Hovhannisyan V., Tunlid A., Velagapudi R., Vision T.J., Zeng Q., Zolan M.E., Pukkila P.J.
      Proc. Natl. Acad. Sci. U.S.A. 107:11889-11894(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003.

    Entry informationi

    Entry nameiLIPA_COPC7
    AccessioniPrimary (citable) accession number: A8N1T1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3