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A8MYU2

- KCNU1_HUMAN

UniProt

A8MYU2 - KCNU1_HUMAN

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Protein

Potassium channel subfamily U member 1

Gene

KCNU1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K+. May represent the primary spermatozoan K+ current. In contrast to KCNMA1/SLO1, it is not activated by Ca2+ or Mg2+. Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.1 Publication

GO - Molecular functioni

  1. large conductance calcium-activated potassium channel activity Source: RefGenome
  2. potassium channel activity Source: Reactome
  3. voltage-gated potassium channel activity Source: RefGenome

GO - Biological processi

  1. multicellular organism reproduction Source: Reactome
  2. potassium ion transmembrane transport Source: RefGenome
  3. single fertilization Source: Reactome
  4. sperm-egg recognition Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_163875. Sperm Motility And Taxes.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily U member 1
Alternative name(s):
Calcium-activated potassium channel subunit alpha-3
Calcium-activated potassium channel, subfamily M subunit alpha-3
KCa5
Slowpoke homolog 3
Gene namesi
Name:KCNU1
Synonyms:KCNMA3, KCNMC1, SLO3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:18867. KCNU1.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity

GO - Cellular componenti

  1. plasma membrane Source: Reactome
  2. voltage-gated potassium channel complex Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38727.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11491149Potassium channel subfamily U member 1PRO_0000349186Add
BLAST

Proteomic databases

PaxDbiA8MYU2.
PRIDEiA8MYU2.

PTM databases

PhosphoSiteiA8MYU2.

Expressioni

Tissue specificityi

Testis-specific.1 Publication

Gene expression databases

BgeeiA8MYU2.
CleanExiHS_KCNU1.
ExpressionAtlasiA8MYU2. baseline and differential.
GenevestigatoriA8MYU2.

Interactioni

Subunit structurei

Homotetramer; which constitutes the calcium-activated potassium channel. May interact with LRRC52; this interaction may change channel gating properties, such as shifting gating to more negative potentials at a given pH.By similarity

Protein-protein interaction databases

DIPiDIP-60091N.
STRINGi9606.ENSP00000382770.

Structurei

Secondary structure

1
1149
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi333 – 3386Combined sources
Helixi342 – 3498Combined sources
Beta strandi363 – 3653Combined sources
Turni382 – 3854Combined sources
Beta strandi386 – 3883Combined sources
Helixi397 – 4037Combined sources
Helixi405 – 4073Combined sources
Beta strandi408 – 4136Combined sources
Helixi422 – 43918Combined sources
Beta strandi445 – 4484Combined sources
Helixi452 – 4565Combined sources
Helixi457 – 4604Combined sources
Turni466 – 4694Combined sources
Beta strandi471 – 4733Combined sources
Helixi475 – 48814Combined sources
Helixi492 – 4987Combined sources
Helixi512 – 52110Combined sources
Beta strandi527 – 5293Combined sources
Helixi532 – 5343Combined sources
Helixi539 – 54911Combined sources
Beta strandi553 – 5586Combined sources
Beta strandi571 – 5733Combined sources
Beta strandi585 – 5917Combined sources
Helixi593 – 5975Combined sources
Helixi598 – 6003Combined sources
Beta strandi685 – 6906Combined sources
Helixi697 – 7004Combined sources
Beta strandi716 – 7205Combined sources
Helixi732 – 7354Combined sources
Helixi736 – 7394Combined sources
Helixi745 – 7473Combined sources
Beta strandi751 – 7555Combined sources
Helixi757 – 7637Combined sources
Helixi764 – 7674Combined sources
Beta strandi771 – 7788Combined sources
Helixi783 – 7886Combined sources
Helixi791 – 7933Combined sources
Beta strandi795 – 8006Combined sources
Helixi815 – 82511Combined sources
Beta strandi861 – 8644Combined sources
Helixi868 – 8703Combined sources
Helixi871 – 8788Combined sources
Turni882 – 8843Combined sources
Helixi889 – 8913Combined sources
Helixi893 – 8975Combined sources
Beta strandi899 – 9013Combined sources
Helixi903 – 9086Combined sources
Helixi909 – 9157Combined sources
Helixi917 – 92812Combined sources
Beta strandi962 – 9665Combined sources
Beta strandi968 – 9714Combined sources
Helixi972 – 9754Combined sources
Helixi982 – 99312Combined sources
Beta strandi996 – 10038Combined sources
Beta strandi1015 – 10206Combined sources
Beta strandi1032 – 10376Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HPFX-ray3.40A/B330-1062[»]
ProteinModelPortaliA8MYU2.
SMRiA8MYU2. Positions 104-315, 331-601, 652-1043.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424ExtracellularSequence AnalysisAdd
BLAST
Topological domaini46 – 10156CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini123 – 13816ExtracellularSequence AnalysisAdd
BLAST
Topological domaini160 – 1634CytoplasmicSequence Analysis
Topological domaini185 – 1884ExtracellularSequence Analysis
Topological domaini210 – 22617CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini248 – 25912ExtracellularSequence AnalysisAdd
BLAST
Topological domaini283 – 2919ExtracellularSequence Analysis
Topological domaini313 – 1149837CytoplasmicSequence AnalysisAdd
BLAST

Intramembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei260 – 28223Pore-forming; Name=P regionSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei25 – 4521Helical; Name=Segment S0Sequence AnalysisAdd
BLAST
Transmembranei102 – 12221Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Transmembranei139 – 15921Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Transmembranei164 – 18421Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Transmembranei189 – 20921Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Transmembranei227 – 24721Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Transmembranei292 – 31221Helical; Name=Segment S6Sequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini339 – 482144RCK N-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni480 – 50021Segment S7Add
BLAST
Regioni537 – 55721Segment S8Add
BLAST
Regioni711 – 73121Segment S9Add
BLAST
Regioni895 – 91521Segment S10Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi276 – 2794Selectivity for potassium

Domaini

The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.By similarity
The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium (By similarity).By similarity
The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel.By similarity
The C-terminal cytosolic region confers the pH-dependence.By similarity

Sequence similaritiesi

Contains 1 RCK N-terminal domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253173.
GeneTreeiENSGT00530000063026.
HOGENOMiHOG000019856.
HOVERGENiHBG052222.
InParanoidiA8MYU2.
KOiK05274.
OMAiSGMFHWC.
OrthoDBiEOG7288R0.
PhylomeDBiA8MYU2.
TreeFamiTF314283.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005821. Ion_trans_dom.
IPR003929. K_chnl_Ca-activ_BK_asu.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF03493. BK_channel_a. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01449. BKCHANNELA.

Sequencei

Sequence statusi: Complete.

A8MYU2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFQTKLRNET WEDLPKMSCT TEIQAAFILS SFVTFFSGLI ILLIFRLIWR
60 70 80 90 100
SVKKWQIIKG TGIILELFTS GTIARSHVRS LHFQGQFRDH IEMLLSAQTF
110 120 130 140 150
VGQVLVILVF VLSIGSLIIY FINSADPVGS CSSYEDKTIP IDLVFNAFFS
160 170 180 190 200
FYFGLRFMAA DDKIKFWLEM NSIVDIFTIP PTFISYYLKS NWLGLRFLRA
210 220 230 240 250
LRLLELPQIL QILRAIKTSN SVKFSKLLSI ILSTWFTAAG FIHLVENSGD
260 270 280 290 300
PWLKGRNSQN ISYFESIYLV MATTSTVGFG DVVAKTSLGR TFIMFFTLGS
310 320 330 340 350
LILFANYIPE MVELFANKRK YTSSYEALKG KKFIVVCGNI TVDSVTAFLR
360 370 380 390 400
NFLRDKSGEI NTEIVFLGET PPSLELETIF KCYLAYTTFI SGSAMKWEDL
410 420 430 440 450
RRVAVESAEA CLIIANPLCS DSHAEDISNI MRVLSIKNYD STTRIIIQIL
460 470 480 490 500
QSHNKVYLPK IPSWNWDTGD NIICFAELKL GFIAQGCLVP GLCTFLTSLF
510 520 530 540 550
VEQNKKVMPK QTWKKHFLNS MKNKILTQRL SDDFAGMSFP EVARLCFLKM
560 570 580 590 600
HLLLIAIEYK SLFTDGFCGL ILNPPPQVRI RKNTLGFFIA ETPKDVRRAL
610 620 630 640 650
FYCSVCHDDV FIPELITNCG CKSRSRQHIT VPSVKRMKKC LKGISSRISG
660 670 680 690 700
QDSPPRVSAS TSSISNFTTR TLQHDVEQDS DQLDSSGMFH WCKPTSLDKV
710 720 730 740 750
TLKRTGKSKY KFRNHIVACV FGDAHSAPMG LRNFVMPLRA SNYTRKELKD
760 770 780 790 800
IVFIGSLDYL QREWRFLWNF PQIYILPGCA LYSGDLHAAN IEQCSMCAVL
810 820 830 840 850
SPPPQPSSNQ TLVDTEAIMA TLTIGSLQID SSSDPSPSVS EETPGYTNGH
860 870 880 890 900
NEKSNCRKVP ILTELKNPSN IHFIEQLGGL EGSLQETNLH LSTAFSTGTV
910 920 930 940 950
FSGSFLDSLL ATAFYNYHVL ELLQMLVTGG VSSQLEQHLD KDKVYGVADS
960 970 980 990 1000
CTSLLSGRNR CKLGLLSLHE TILSDVNPRN TFGQLFCGSL DLFGILCVGL
1010 1020 1030 1040 1050
YRIIDEEELN PENKRFVITR PANEFKLLPS DLVFCAIPFS TACYKRNEEF
1060 1070 1080 1090 1100
SLQKSYEIVN KASQTTETHS DTNCPPTIDS VTETLYSPVY SYQPRTNSLS
1110 1120 1130 1140
FPKQIAWNQS RTNSIISSQI PLGDNAKENE RKTSDEVYDE DPFAYSEPL
Length:1,149
Mass (Da):129,543
Last modified:September 2, 2008 - v2
Checksum:iBA527F41498B6AA7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti175 – 1751D → N.
Corresponds to variant rs1111125 [ dbSNP | Ensembl ].
VAR_060148
Natural varianti768 – 7681W → R.
Corresponds to variant rs28608091 [ dbSNP | Ensembl ].
VAR_053868
Natural varianti916 – 9161N → S.
Corresponds to variant rs16885577 [ dbSNP | Ensembl ].
VAR_053869

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC124076 Genomic DNA. No translation available.
CCDSiCCDS55220.1.
RefSeqiNP_001027006.2. NM_001031836.2.
UniGeneiHs.13861.

Genome annotation databases

EnsembliENST00000399881; ENSP00000382770; ENSG00000215262.
GeneIDi157855.
KEGGihsa:157855.
UCSCiuc010lvw.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC124076 Genomic DNA. No translation available.
CCDSi CCDS55220.1.
RefSeqi NP_001027006.2. NM_001031836.2.
UniGenei Hs.13861.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HPF X-ray 3.40 A/B 330-1062 [» ]
ProteinModelPortali A8MYU2.
SMRi A8MYU2. Positions 104-315, 331-601, 652-1043.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60091N.
STRINGi 9606.ENSP00000382770.

Chemistry

GuidetoPHARMACOLOGYi 387.

PTM databases

PhosphoSitei A8MYU2.

Proteomic databases

PaxDbi A8MYU2.
PRIDEi A8MYU2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000399881 ; ENSP00000382770 ; ENSG00000215262 .
GeneIDi 157855.
KEGGi hsa:157855.
UCSCi uc010lvw.3. human.

Organism-specific databases

CTDi 157855.
GeneCardsi GC08P036641.
H-InvDB HIX0020845.
HGNCi HGNC:18867. KCNU1.
MIMi 615215. gene.
neXtProti NX_A8MYU2.
PharmGKBi PA38727.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG253173.
GeneTreei ENSGT00530000063026.
HOGENOMi HOG000019856.
HOVERGENi HBG052222.
InParanoidi A8MYU2.
KOi K05274.
OMAi SGMFHWC.
OrthoDBi EOG7288R0.
PhylomeDBi A8MYU2.
TreeFami TF314283.

Enzyme and pathway databases

Reactomei REACT_163875. Sperm Motility And Taxes.

Miscellaneous databases

GeneWikii KCNU1.
GenomeRNAii 157855.
NextBioi 87566.
PROi A8MYU2.
SOURCEi Search...

Gene expression databases

Bgeei A8MYU2.
CleanExi HS_KCNU1.
ExpressionAtlasi A8MYU2. baseline and differential.
Genevestigatori A8MYU2.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR005821. Ion_trans_dom.
IPR003929. K_chnl_Ca-activ_BK_asu.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF03493. BK_channel_a. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
PRINTSi PR01449. BKCHANNELA.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Slo3, a novel pH-sensitive K+ channel from mammalian spermatocytes."
    Schreiber M., Wei A., Yuan A., Gaut J., Saito M., Salkoff L.
    J. Biol. Chem. 273:3509-3516(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. "Functional and structural analysis of the human SLO3 pH- and voltage-gated K+ channel."
    Leonetti M.D., Yuan P., Hsiung Y., Mackinnon R.
    Proc. Natl. Acad. Sci. U.S.A. 109:19274-19279(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 330-1063, FUNCTION, PH DEPENDENCE.

Entry informationi

Entry nameiKCNU1_HUMAN
AccessioniPrimary (citable) accession number: A8MYU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: October 29, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3