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A8MYU2

- KCNU1_HUMAN

UniProt

A8MYU2 - KCNU1_HUMAN

Protein

Potassium channel subfamily U member 1

Gene

KCNU1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (02 Sep 2008)
      Previous versions | rss
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    Functioni

    Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K+. May represent the primary spermatozoan K+ current. In contrast to KCNMA1/SLO1, it is not activated by Ca2+ or Mg2+. Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.1 Publication

    GO - Molecular functioni

    1. large conductance calcium-activated potassium channel activity Source: RefGenome
    2. potassium channel activity Source: Reactome
    3. voltage-gated potassium channel activity Source: RefGenome

    GO - Biological processi

    1. multicellular organism reproduction Source: Reactome
    2. potassium ion transmembrane transport Source: RefGenome
    3. single fertilization Source: Reactome
    4. sperm-egg recognition Source: Reactome

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_163875. Sperm Motility And Taxes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel subfamily U member 1
    Alternative name(s):
    Calcium-activated potassium channel subunit alpha-3
    Calcium-activated potassium channel, subfamily M subunit alpha-3
    KCa5
    Slowpoke homolog 3
    Gene namesi
    Name:KCNU1
    Synonyms:KCNMA3, KCNMC1, SLO3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:18867. KCNU1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. plasma membrane Source: Reactome
    2. voltage-gated potassium channel complex Source: RefGenome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA38727.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11491149Potassium channel subfamily U member 1PRO_0000349186Add
    BLAST

    Proteomic databases

    PaxDbiA8MYU2.
    PRIDEiA8MYU2.

    PTM databases

    PhosphoSiteiA8MYU2.

    Expressioni

    Tissue specificityi

    Testis-specific.1 Publication

    Gene expression databases

    ArrayExpressiA8MYU2.
    BgeeiA8MYU2.
    CleanExiHS_KCNU1.
    GenevestigatoriA8MYU2.

    Interactioni

    Subunit structurei

    Homotetramer; which constitutes the calcium-activated potassium channel. May interact with LRRC52; this interaction may change channel gating properties, such as shifting gating to more negative potentials at a given pH.By similarity

    Protein-protein interaction databases

    DIPiDIP-60091N.
    STRINGi9606.ENSP00000382770.

    Structurei

    Secondary structure

    1
    1149
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi333 – 3386
    Helixi342 – 3498
    Beta strandi363 – 3653
    Turni382 – 3854
    Beta strandi386 – 3883
    Helixi397 – 4037
    Helixi405 – 4073
    Beta strandi408 – 4136
    Helixi422 – 43918
    Beta strandi445 – 4484
    Helixi452 – 4565
    Helixi457 – 4604
    Turni466 – 4694
    Beta strandi471 – 4733
    Helixi475 – 48814
    Helixi492 – 4987
    Helixi512 – 52110
    Beta strandi527 – 5293
    Helixi532 – 5343
    Helixi539 – 54911
    Beta strandi553 – 5586
    Beta strandi571 – 5733
    Beta strandi585 – 5917
    Helixi593 – 5975
    Helixi598 – 6003
    Beta strandi685 – 6906
    Helixi697 – 7004
    Beta strandi716 – 7205
    Helixi732 – 7354
    Helixi736 – 7394
    Helixi745 – 7473
    Beta strandi751 – 7555
    Helixi757 – 7637
    Helixi764 – 7674
    Beta strandi771 – 7788
    Helixi783 – 7886
    Helixi791 – 7933
    Beta strandi795 – 8006
    Helixi815 – 82511
    Beta strandi861 – 8644
    Helixi868 – 8703
    Helixi871 – 8788
    Turni882 – 8843
    Helixi889 – 8913
    Helixi893 – 8975
    Beta strandi899 – 9013
    Helixi903 – 9086
    Helixi909 – 9157
    Helixi917 – 92812
    Beta strandi962 – 9665
    Beta strandi968 – 9714
    Helixi972 – 9754
    Helixi982 – 99312
    Beta strandi996 – 10038
    Beta strandi1015 – 10206
    Beta strandi1032 – 10376

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HPFX-ray3.40A/B330-1062[»]
    ProteinModelPortaliA8MYU2.
    SMRiA8MYU2. Positions 104-315, 331-601, 652-1043.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini46 – 10156CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini123 – 13816ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini160 – 1634CytoplasmicSequence Analysis
    Topological domaini185 – 1884ExtracellularSequence Analysis
    Topological domaini210 – 22617CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini248 – 25912ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini283 – 2919ExtracellularSequence Analysis
    Topological domaini313 – 1149837CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei260 – 28223Pore-forming; Name=P regionSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4521Helical; Name=Segment S0Sequence AnalysisAdd
    BLAST
    Transmembranei102 – 12221Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei139 – 15921Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei164 – 18421Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei189 – 20921Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei227 – 24721Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei292 – 31221Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini339 – 482144RCK N-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni480 – 50021Segment S7Add
    BLAST
    Regioni537 – 55721Segment S8Add
    BLAST
    Regioni711 – 73121Segment S9Add
    BLAST
    Regioni895 – 91521Segment S10Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi276 – 2794Selectivity for potassium

    Domaini

    The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.By similarity
    The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium By similarity.By similarity
    The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel.By similarity
    The C-terminal cytosolic region confers the pH-dependence.By similarity

    Sequence similaritiesi

    Contains 1 RCK N-terminal domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG253173.
    HOGENOMiHOG000019856.
    HOVERGENiHBG052222.
    KOiK05274.
    OMAiSGMFHWC.
    OrthoDBiEOG7288R0.
    PhylomeDBiA8MYU2.
    TreeFamiTF314283.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR005821. Ion_trans_dom.
    IPR003929. K_chnl_Ca-activ_BK_asu.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF03493. BK_channel_a. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR01449. BKCHANNELA.

    Sequencei

    Sequence statusi: Complete.

    A8MYU2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFQTKLRNET WEDLPKMSCT TEIQAAFILS SFVTFFSGLI ILLIFRLIWR     50
    SVKKWQIIKG TGIILELFTS GTIARSHVRS LHFQGQFRDH IEMLLSAQTF 100
    VGQVLVILVF VLSIGSLIIY FINSADPVGS CSSYEDKTIP IDLVFNAFFS 150
    FYFGLRFMAA DDKIKFWLEM NSIVDIFTIP PTFISYYLKS NWLGLRFLRA 200
    LRLLELPQIL QILRAIKTSN SVKFSKLLSI ILSTWFTAAG FIHLVENSGD 250
    PWLKGRNSQN ISYFESIYLV MATTSTVGFG DVVAKTSLGR TFIMFFTLGS 300
    LILFANYIPE MVELFANKRK YTSSYEALKG KKFIVVCGNI TVDSVTAFLR 350
    NFLRDKSGEI NTEIVFLGET PPSLELETIF KCYLAYTTFI SGSAMKWEDL 400
    RRVAVESAEA CLIIANPLCS DSHAEDISNI MRVLSIKNYD STTRIIIQIL 450
    QSHNKVYLPK IPSWNWDTGD NIICFAELKL GFIAQGCLVP GLCTFLTSLF 500
    VEQNKKVMPK QTWKKHFLNS MKNKILTQRL SDDFAGMSFP EVARLCFLKM 550
    HLLLIAIEYK SLFTDGFCGL ILNPPPQVRI RKNTLGFFIA ETPKDVRRAL 600
    FYCSVCHDDV FIPELITNCG CKSRSRQHIT VPSVKRMKKC LKGISSRISG 650
    QDSPPRVSAS TSSISNFTTR TLQHDVEQDS DQLDSSGMFH WCKPTSLDKV 700
    TLKRTGKSKY KFRNHIVACV FGDAHSAPMG LRNFVMPLRA SNYTRKELKD 750
    IVFIGSLDYL QREWRFLWNF PQIYILPGCA LYSGDLHAAN IEQCSMCAVL 800
    SPPPQPSSNQ TLVDTEAIMA TLTIGSLQID SSSDPSPSVS EETPGYTNGH 850
    NEKSNCRKVP ILTELKNPSN IHFIEQLGGL EGSLQETNLH LSTAFSTGTV 900
    FSGSFLDSLL ATAFYNYHVL ELLQMLVTGG VSSQLEQHLD KDKVYGVADS 950
    CTSLLSGRNR CKLGLLSLHE TILSDVNPRN TFGQLFCGSL DLFGILCVGL 1000
    YRIIDEEELN PENKRFVITR PANEFKLLPS DLVFCAIPFS TACYKRNEEF 1050
    SLQKSYEIVN KASQTTETHS DTNCPPTIDS VTETLYSPVY SYQPRTNSLS 1100
    FPKQIAWNQS RTNSIISSQI PLGDNAKENE RKTSDEVYDE DPFAYSEPL 1149
    Length:1,149
    Mass (Da):129,543
    Last modified:September 2, 2008 - v2
    Checksum:iBA527F41498B6AA7
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti175 – 1751D → N.
    Corresponds to variant rs1111125 [ dbSNP | Ensembl ].
    VAR_060148
    Natural varianti768 – 7681W → R.
    Corresponds to variant rs28608091 [ dbSNP | Ensembl ].
    VAR_053868
    Natural varianti916 – 9161N → S.
    Corresponds to variant rs16885577 [ dbSNP | Ensembl ].
    VAR_053869

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC124076 Genomic DNA. No translation available.
    CCDSiCCDS55220.1.
    RefSeqiNP_001027006.2. NM_001031836.2.
    UniGeneiHs.13861.

    Genome annotation databases

    EnsembliENST00000399881; ENSP00000382770; ENSG00000215262.
    GeneIDi157855.
    KEGGihsa:157855.
    UCSCiuc010lvw.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC124076 Genomic DNA. No translation available.
    CCDSi CCDS55220.1.
    RefSeqi NP_001027006.2. NM_001031836.2.
    UniGenei Hs.13861.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HPF X-ray 3.40 A/B 330-1062 [» ]
    ProteinModelPortali A8MYU2.
    SMRi A8MYU2. Positions 104-315, 331-601, 652-1043.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60091N.
    STRINGi 9606.ENSP00000382770.

    Chemistry

    GuidetoPHARMACOLOGYi 387.

    PTM databases

    PhosphoSitei A8MYU2.

    Proteomic databases

    PaxDbi A8MYU2.
    PRIDEi A8MYU2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000399881 ; ENSP00000382770 ; ENSG00000215262 .
    GeneIDi 157855.
    KEGGi hsa:157855.
    UCSCi uc010lvw.3. human.

    Organism-specific databases

    CTDi 157855.
    GeneCardsi GC08P036641.
    H-InvDB HIX0020845.
    HGNCi HGNC:18867. KCNU1.
    MIMi 615215. gene.
    neXtProti NX_A8MYU2.
    PharmGKBi PA38727.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG253173.
    HOGENOMi HOG000019856.
    HOVERGENi HBG052222.
    KOi K05274.
    OMAi SGMFHWC.
    OrthoDBi EOG7288R0.
    PhylomeDBi A8MYU2.
    TreeFami TF314283.

    Enzyme and pathway databases

    Reactomei REACT_163875. Sperm Motility And Taxes.

    Miscellaneous databases

    GeneWikii KCNU1.
    GenomeRNAii 157855.
    NextBioi 87566.
    PROi A8MYU2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A8MYU2.
    Bgeei A8MYU2.
    CleanExi HS_KCNU1.
    Genevestigatori A8MYU2.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR005821. Ion_trans_dom.
    IPR003929. K_chnl_Ca-activ_BK_asu.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF03493. BK_channel_a. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR01449. BKCHANNELA.
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Slo3, a novel pH-sensitive K+ channel from mammalian spermatocytes."
      Schreiber M., Wei A., Yuan A., Gaut J., Saito M., Salkoff L.
      J. Biol. Chem. 273:3509-3516(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    3. "Functional and structural analysis of the human SLO3 pH- and voltage-gated K+ channel."
      Leonetti M.D., Yuan P., Hsiung Y., Mackinnon R.
      Proc. Natl. Acad. Sci. U.S.A. 109:19274-19279(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 330-1063, FUNCTION, PH DEPENDENCE.

    Entry informationi

    Entry nameiKCNU1_HUMAN
    AccessioniPrimary (citable) accession number: A8MYU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3