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A8MX76 (CAN14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calpain-14

EC=3.4.22.-
Alternative name(s):
Calcium-activated neutral proteinase 14
Short name=CANP 14
Gene names
Name:CAPN14
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length684 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-regulated non-lysosomal thiol-protease By similarity.

Tissue specificity

Not expressed in tissues tested. Ref.3

Sequence similarities

Belongs to the peptidase C2 family.

Contains 1 calpain catalytic domain.

Contains 3 EF-hand domains.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentcytoplasm

Inferred from direct assay. Source: HPA

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

calcium-dependent cysteine-type endopeptidase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A8MX76-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A8MX76-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-176: Missing.
     177-184: LLEKAYAK → MLASSGSG

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 684684Calpain-14
PRO_0000341373

Regions

Domain43 – 336294Calpain catalytic
Domain557 – 59236EF-hand 1
Domain586 – 62136EF-hand 2
Domain651 – 68434EF-hand 3
Calcium binding570 – 58112 Potential
Region337 – 503167Domain III
Region504 – 51714Linker
Region518 – 683166Domain IV

Sites

Active site1011 By similarity
Active site2541 By similarity
Active site2781 By similarity

Natural variations

Alternative sequence1 – 176176Missing in isoform 2.
VSP_034268
Alternative sequence177 – 1848LLEKAYAK → MLASSGSG in isoform 2.
VSP_034269

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 10, 2008. Version 2.
Checksum: 6627B859E4F375A9

FASTA68479,568
        10         20         30         40         50         60 
MSLWPPFRCR WKLAPRYSRR ASPQQPQQDF EALLAECLRN GCLFEDTSFP ATLSSIGSGS 

        70         80         90        100        110        120 
LLQKLPPRLQ WKRPPELHSN PQFYFAKAKR LDLCQGIVGD CWFLAALQAL ALHQDILSRV 

       130        140        150        160        170        180 
VPLNQSFTEK YAGIFRFWFW HYGNWVPVVI DDRLPVNEAG QLVFVSSTYK NLFWGALLEK 

       190        200        210        220        230        240 
AYAKLSGSYE DLQSGQVSEA LVDFTGGVTM TINLAEAHGN LWDILIEATY NRTLIGCQTH 

       250        260        270        280        290        300 
SGEKILENGL VEGHAYTLTG IRKVTCKHRP EYLVKLRNPW GKVEWKGDWS DSSSKWELLS 

       310        320        330        340        350        360 
PKEKILLLRK DNDGEFWMTL QDFKTHFVLL VICKLTPGLL SQEAAQKWTY TMREGRWEKR 

       370        380        390        400        410        420 
STAGGQRQLL QDTFWKNPQF LLSVWRPEEG RRSLRPCSVL VSLLQKPRHR CRKRKPLLAI 

       430        440        450        460        470        480 
GFYLYRMNKY HDDQRRLPPE FFQRNTPLSQ PDRFLKEKEV SQELCLEPGT YLIVPCILEA 

       490        500        510        520        530        540 
HQKSEFVLRV FSRKHIFYEI GSNSGVVFSK EIEDQNERQD EFFTKFFEKH PEINAVQLQN 

       550        560        570        580        590        600 
LLNQMTWSSL GSRQPFFSLE ACQGILALLD LNASGTMSIQ EFRDLWKQLK LSQKVFHKQD 

       610        620        630        640        650        660 
RGSGYLNWEQ LHAAMREAGI MLSDDVCQLM LIRYGGPRLQ MDFVSFIHLM LRVENMEDVF 

       670        680 
QNLTQDGKGI YLQKPEWMMM ALYS 

« Hide

Isoform 2 [UniParc].

Checksum: F8DBD839E18AC7F7
Show »

FASTA50859,025

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Teratocarcinoma.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Identification and characterization of two novel calpain large subunit genes."
Dear T.N., Boehm T.
Gene 274:245-252(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, LACK OF TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK092257 mRNA. Translation: BAG52511.1.
AC015980 Genomic DNA. No translation available.
CCDSCCDS46254.1. [A8MX76-1]
RefSeqNP_001138594.1. NM_001145122.1. [A8MX76-1]
XP_006712085.1. XM_006712022.1. [A8MX76-2]
UniGeneHs.468059.

3D structure databases

ProteinModelPortalA8MX76.
SMRA8MX76. Positions 28-682.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000385247.

Protein family/group databases

MEROPSC02.021.

PTM databases

PhosphoSiteA8MX76.

Proteomic databases

PaxDbA8MX76.
PRIDEA8MX76.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000403897; ENSP00000385247; ENSG00000214711. [A8MX76-1]
GeneID440854.
KEGGhsa:440854.
UCSCuc002rnt.2. human. [A8MX76-2]
uc010yms.2. human. [A8MX76-1]

Organism-specific databases

CTD440854.
GeneCardsGC02M031370.
H-InvDBHIX0001949.
HGNCHGNC:16664. CAPN14.
HPAHPA035720.
HPA035721.
MIM610229. gene.
neXtProtNX_A8MX76.
PharmGKBPA134888839.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG249866.
HOGENOMHOG000232035.
HOVERGENHBG012645.
KOK13442.
OrthoDBEOG7RV9FM.
PhylomeDBA8MX76.
TreeFamTF314748.

Gene expression databases

ArrayExpressA8MX76.
BgeeA8MX76.
CleanExHS_CAPN14.
GenevestigatorA8MX76.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSPR00704. CALPAIN.
SMARTSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMSSF49758. SSF49758. 1 hit.
PROSITEPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio109543.
PROA8MX76.
SOURCESearch...

Entry information

Entry nameCAN14_HUMAN
AccessionPrimary (citable) accession number: A8MX76
Secondary accession number(s): B3KRU9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM