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A8MX76

- CAN14_HUMAN

UniProt

A8MX76 - CAN14_HUMAN

Protein

Calpain-14

Gene

CAPN14

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 2 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei101 – 1011By similarity
    Active sitei254 – 2541By similarity
    Active sitei278 – 2781By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi570 – 58112PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: RefGenome
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiC02.021.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-14 (EC:3.4.22.-)
    Alternative name(s):
    Calcium-activated neutral proteinase 14
    Short name:
    CANP 14
    Gene namesi
    Name:CAPN14
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:16664. CAPN14.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. nucleus Source: HPA

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134888839.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 684684Calpain-14PRO_0000341373Add
    BLAST

    Proteomic databases

    PaxDbiA8MX76.
    PRIDEiA8MX76.

    PTM databases

    PhosphoSiteiA8MX76.

    Expressioni

    Tissue specificityi

    Not expressed in tissues tested.

    Gene expression databases

    ArrayExpressiA8MX76.
    BgeeiA8MX76.
    CleanExiHS_CAPN14.
    GenevestigatoriA8MX76.

    Organism-specific databases

    HPAiHPA035720.
    HPA035721.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000385247.

    Structurei

    3D structure databases

    ProteinModelPortaliA8MX76.
    SMRiA8MX76. Positions 28-682.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 336294Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini557 – 59236EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini586 – 62136EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini651 – 68434EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni337 – 503167Domain IIIAdd
    BLAST
    Regioni504 – 51714LinkerAdd
    BLAST
    Regioni518 – 683166Domain IVAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 3 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG249866.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.
    KOiK13442.
    OrthoDBiEOG7RV9FM.
    PhylomeDBiA8MX76.
    TreeFamiTF314748.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PfamiPF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A8MX76-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLWPPFRCR WKLAPRYSRR ASPQQPQQDF EALLAECLRN GCLFEDTSFP    50
    ATLSSIGSGS LLQKLPPRLQ WKRPPELHSN PQFYFAKAKR LDLCQGIVGD 100
    CWFLAALQAL ALHQDILSRV VPLNQSFTEK YAGIFRFWFW HYGNWVPVVI 150
    DDRLPVNEAG QLVFVSSTYK NLFWGALLEK AYAKLSGSYE DLQSGQVSEA 200
    LVDFTGGVTM TINLAEAHGN LWDILIEATY NRTLIGCQTH SGEKILENGL 250
    VEGHAYTLTG IRKVTCKHRP EYLVKLRNPW GKVEWKGDWS DSSSKWELLS 300
    PKEKILLLRK DNDGEFWMTL QDFKTHFVLL VICKLTPGLL SQEAAQKWTY 350
    TMREGRWEKR STAGGQRQLL QDTFWKNPQF LLSVWRPEEG RRSLRPCSVL 400
    VSLLQKPRHR CRKRKPLLAI GFYLYRMNKY HDDQRRLPPE FFQRNTPLSQ 450
    PDRFLKEKEV SQELCLEPGT YLIVPCILEA HQKSEFVLRV FSRKHIFYEI 500
    GSNSGVVFSK EIEDQNERQD EFFTKFFEKH PEINAVQLQN LLNQMTWSSL 550
    GSRQPFFSLE ACQGILALLD LNASGTMSIQ EFRDLWKQLK LSQKVFHKQD 600
    RGSGYLNWEQ LHAAMREAGI MLSDDVCQLM LIRYGGPRLQ MDFVSFIHLM 650
    LRVENMEDVF QNLTQDGKGI YLQKPEWMMM ALYS 684
    Length:684
    Mass (Da):79,568
    Last modified:June 10, 2008 - v2
    Checksum:i6627B859E4F375A9
    GO
    Isoform 2 (identifier: A8MX76-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-176: Missing.
         177-184: LLEKAYAK → MLASSGSG

    Show »
    Length:508
    Mass (Da):59,025
    Checksum:iF8DBD839E18AC7F7
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 176176Missing in isoform 2. 1 PublicationVSP_034268Add
    BLAST
    Alternative sequencei177 – 1848LLEKAYAK → MLASSGSG in isoform 2. 1 PublicationVSP_034269

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK092257 mRNA. Translation: BAG52511.1.
    AC015980 Genomic DNA. No translation available.
    CCDSiCCDS46254.1. [A8MX76-1]
    RefSeqiNP_001138594.1. NM_001145122.1. [A8MX76-1]
    XP_006712085.1. XM_006712022.1. [A8MX76-2]
    UniGeneiHs.468059.

    Genome annotation databases

    EnsembliENST00000403897; ENSP00000385247; ENSG00000214711. [A8MX76-1]
    GeneIDi440854.
    KEGGihsa:440854.
    UCSCiuc002rnt.2. human. [A8MX76-2]
    uc010yms.2. human. [A8MX76-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK092257 mRNA. Translation: BAG52511.1 .
    AC015980 Genomic DNA. No translation available.
    CCDSi CCDS46254.1. [A8MX76-1 ]
    RefSeqi NP_001138594.1. NM_001145122.1. [A8MX76-1 ]
    XP_006712085.1. XM_006712022.1. [A8MX76-2 ]
    UniGenei Hs.468059.

    3D structure databases

    ProteinModelPortali A8MX76.
    SMRi A8MX76. Positions 28-682.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000385247.

    Protein family/group databases

    MEROPSi C02.021.

    PTM databases

    PhosphoSitei A8MX76.

    Proteomic databases

    PaxDbi A8MX76.
    PRIDEi A8MX76.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000403897 ; ENSP00000385247 ; ENSG00000214711 . [A8MX76-1 ]
    GeneIDi 440854.
    KEGGi hsa:440854.
    UCSCi uc002rnt.2. human. [A8MX76-2 ]
    uc010yms.2. human. [A8MX76-1 ]

    Organism-specific databases

    CTDi 440854.
    GeneCardsi GC02M031370.
    H-InvDB HIX0001949.
    HGNCi HGNC:16664. CAPN14.
    HPAi HPA035720.
    HPA035721.
    MIMi 610229. gene.
    neXtProti NX_A8MX76.
    PharmGKBi PA134888839.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG249866.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.
    KOi K13442.
    OrthoDBi EOG7RV9FM.
    PhylomeDBi A8MX76.
    TreeFami TF314748.

    Miscellaneous databases

    NextBioi 109543.
    PROi A8MX76.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A8MX76.
    Bgeei A8MX76.
    CleanExi HS_CAPN14.
    Genevestigatori A8MX76.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    Pfami PF01067. Calpain_III. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Teratocarcinoma.
    2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Identification and characterization of two novel calpain large subunit genes."
      Dear T.N., Boehm T.
      Gene 274:245-252(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, LACK OF TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCAN14_HUMAN
    AccessioniPrimary (citable) accession number: A8MX76
    Secondary accession number(s): B3KRU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 64 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3