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Protein

Calpain-14

Gene

CAPN14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101By similarity1
Active sitei254By similarity1
Active sitei278By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi570 – 581PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.

Protein family/group databases

MEROPSiC02.021.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-14 (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase 14
Short name:
CANP 14
Gene namesi
Name:CAPN14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:16664. CAPN14.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi440854.
OpenTargetsiENSG00000214711.
PharmGKBiPA134888839.

Polymorphism and mutation databases

BioMutaiCAPN14.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003413731 – 684Calpain-14Add BLAST684

Proteomic databases

MaxQBiA8MX76.
PaxDbiA8MX76.
PRIDEiA8MX76.

PTM databases

iPTMnetiA8MX76.
PhosphoSitePlusiA8MX76.

Expressioni

Tissue specificityi

Not expressed in tissues tested.

Gene expression databases

BgeeiENSG00000214711.
CleanExiHS_CAPN14.
ExpressionAtlasiA8MX76. baseline and differential.

Organism-specific databases

HPAiHPA035720.
HPA035721.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000385247.

Structurei

3D structure databases

ProteinModelPortaliA8MX76.
SMRiA8MX76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 336Calpain catalyticPROSITE-ProRule annotationAdd BLAST294
Domaini557 – 592EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini586 – 621EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini651 – 684EF-hand 3PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni337 – 503Domain IIIAdd BLAST167
Regioni504 – 517LinkerAdd BLAST14
Regioni518 – 683Domain IVAdd BLAST166

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiA8MX76.
KOiK13442.
OMAiQLMLIRY.
OrthoDBiEOG091G049E.
PhylomeDBiA8MX76.
TreeFamiTF314748.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A8MX76-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLWPPFRCR WKLAPRYSRR ASPQQPQQDF EALLAECLRN GCLFEDTSFP
60 70 80 90 100
ATLSSIGSGS LLQKLPPRLQ WKRPPELHSN PQFYFAKAKR LDLCQGIVGD
110 120 130 140 150
CWFLAALQAL ALHQDILSRV VPLNQSFTEK YAGIFRFWFW HYGNWVPVVI
160 170 180 190 200
DDRLPVNEAG QLVFVSSTYK NLFWGALLEK AYAKLSGSYE DLQSGQVSEA
210 220 230 240 250
LVDFTGGVTM TINLAEAHGN LWDILIEATY NRTLIGCQTH SGEKILENGL
260 270 280 290 300
VEGHAYTLTG IRKVTCKHRP EYLVKLRNPW GKVEWKGDWS DSSSKWELLS
310 320 330 340 350
PKEKILLLRK DNDGEFWMTL QDFKTHFVLL VICKLTPGLL SQEAAQKWTY
360 370 380 390 400
TMREGRWEKR STAGGQRQLL QDTFWKNPQF LLSVWRPEEG RRSLRPCSVL
410 420 430 440 450
VSLLQKPRHR CRKRKPLLAI GFYLYRMNKY HDDQRRLPPE FFQRNTPLSQ
460 470 480 490 500
PDRFLKEKEV SQELCLEPGT YLIVPCILEA HQKSEFVLRV FSRKHIFYEI
510 520 530 540 550
GSNSGVVFSK EIEDQNERQD EFFTKFFEKH PEINAVQLQN LLNQMTWSSL
560 570 580 590 600
GSRQPFFSLE ACQGILALLD LNASGTMSIQ EFRDLWKQLK LSQKVFHKQD
610 620 630 640 650
RGSGYLNWEQ LHAAMREAGI MLSDDVCQLM LIRYGGPRLQ MDFVSFIHLM
660 670 680
LRVENMEDVF QNLTQDGKGI YLQKPEWMMM ALYS
Length:684
Mass (Da):79,568
Last modified:June 10, 2008 - v2
Checksum:i6627B859E4F375A9
GO
Isoform 2 (identifier: A8MX76-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-176: Missing.
     177-184: LLEKAYAK → MLASSGSG

Show »
Length:508
Mass (Da):59,025
Checksum:iF8DBD839E18AC7F7
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0342681 – 176Missing in isoform 2. 1 PublicationAdd BLAST176
Alternative sequenceiVSP_034269177 – 184LLEKAYAK → MLASSGSG in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK092257 mRNA. Translation: BAG52511.1.
AC015980 Genomic DNA. No translation available.
CCDSiCCDS46254.1. [A8MX76-1]
RefSeqiNP_001138594.1. NM_001145122.1. [A8MX76-1]
NP_001308199.1. NM_001321270.1. [A8MX76-2]
XP_011531165.1. XM_011532863.2. [A8MX76-1]
XP_011531166.1. XM_011532864.2. [A8MX76-1]
XP_011531167.1. XM_011532865.1. [A8MX76-1]
XP_011531168.1. XM_011532866.2. [A8MX76-2]
UniGeneiHs.468059.

Genome annotation databases

EnsembliENST00000403897; ENSP00000385247; ENSG00000214711. [A8MX76-1]
GeneIDi440854.
KEGGihsa:440854.
UCSCiuc010yms.3. human. [A8MX76-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK092257 mRNA. Translation: BAG52511.1.
AC015980 Genomic DNA. No translation available.
CCDSiCCDS46254.1. [A8MX76-1]
RefSeqiNP_001138594.1. NM_001145122.1. [A8MX76-1]
NP_001308199.1. NM_001321270.1. [A8MX76-2]
XP_011531165.1. XM_011532863.2. [A8MX76-1]
XP_011531166.1. XM_011532864.2. [A8MX76-1]
XP_011531167.1. XM_011532865.1. [A8MX76-1]
XP_011531168.1. XM_011532866.2. [A8MX76-2]
UniGeneiHs.468059.

3D structure databases

ProteinModelPortaliA8MX76.
SMRiA8MX76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000385247.

Protein family/group databases

MEROPSiC02.021.

PTM databases

iPTMnetiA8MX76.
PhosphoSitePlusiA8MX76.

Polymorphism and mutation databases

BioMutaiCAPN14.

Proteomic databases

MaxQBiA8MX76.
PaxDbiA8MX76.
PRIDEiA8MX76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000403897; ENSP00000385247; ENSG00000214711. [A8MX76-1]
GeneIDi440854.
KEGGihsa:440854.
UCSCiuc010yms.3. human. [A8MX76-1]

Organism-specific databases

CTDi440854.
DisGeNETi440854.
GeneCardsiCAPN14.
H-InvDBHIX0001949.
HGNCiHGNC:16664. CAPN14.
HPAiHPA035720.
HPA035721.
MIMi610229. gene.
neXtProtiNX_A8MX76.
OpenTargetsiENSG00000214711.
PharmGKBiPA134888839.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiA8MX76.
KOiK13442.
OMAiQLMLIRY.
OrthoDBiEOG091G049E.
PhylomeDBiA8MX76.
TreeFamiTF314748.

Enzyme and pathway databases

ReactomeiR-HSA-1474228. Degradation of the extracellular matrix.

Miscellaneous databases

ChiTaRSiCAPN14. human.
GenomeRNAii440854.
PROiA8MX76.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000214711.
CleanExiHS_CAPN14.
ExpressionAtlasiA8MX76. baseline and differential.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN14_HUMAN
AccessioniPrimary (citable) accession number: A8MX76
Secondary accession number(s): B3KRU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: November 30, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.