A8MX76 (CAN14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calpain-14
EC=3.4.22.-

Alternative name(s):
Calcium-activated neutral proteinase 14
Short name=CANP 14

Gene names

Name:

CAPN14

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calcium-regulated non-lysosomal thiol-protease By similarity.
Tissue specificity	Not expressed in tissues tested. Ref.3
Sequence similarities	Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 3 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A8MX76-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A8MX76-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-176: Missing.
     177-184: LLEKAYAK → MLASSGSG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 684	684	Calpain-14		PRO_0000341373
Regions
	Domain	43 – 336	294	Calpain catalytic
	Domain	557 – 592	36	EF-hand 1
	Domain	586 – 621	36	EF-hand 2
	Domain	651 – 684	34	EF-hand 3
	Calcium binding	570 – 581	12	Potential
	Region	337 – 503	167	Domain III
	Region	504 – 517	14	Linker
	Region	518 – 683	166	Domain IV
Sites
	Active site	101	1	By similarity
	Active site	254	1	By similarity
	Active site	278	1	By similarity
Natural variations
	Alternative sequence	1 – 176	176	Missing in isoform 2.		VSP_034268
	Alternative sequence	177 – 184	8	LLEKAYAK → MLASSGSG in isoform 2.		VSP_034269

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: 6627B859E4F375A9	FASTA	684	79,568
	10 20 30 40 50 60 MSLWPPFRCR WKLAPRYSRR ASPQQPQQDF EALLAECLRN GCLFEDTSFP ATLSSIGSGS 70 80 90 100 110 120 LLQKLPPRLQ WKRPPELHSN PQFYFAKAKR LDLCQGIVGD CWFLAALQAL ALHQDILSRV 130 140 150 160 170 180 VPLNQSFTEK YAGIFRFWFW HYGNWVPVVI DDRLPVNEAG QLVFVSSTYK NLFWGALLEK 190 200 210 220 230 240 AYAKLSGSYE DLQSGQVSEA LVDFTGGVTM TINLAEAHGN LWDILIEATY NRTLIGCQTH 250 260 270 280 290 300 SGEKILENGL VEGHAYTLTG IRKVTCKHRP EYLVKLRNPW GKVEWKGDWS DSSSKWELLS 310 320 330 340 350 360 PKEKILLLRK DNDGEFWMTL QDFKTHFVLL VICKLTPGLL SQEAAQKWTY TMREGRWEKR 370 380 390 400 410 420 STAGGQRQLL QDTFWKNPQF LLSVWRPEEG RRSLRPCSVL VSLLQKPRHR CRKRKPLLAI 430 440 450 460 470 480 GFYLYRMNKY HDDQRRLPPE FFQRNTPLSQ PDRFLKEKEV SQELCLEPGT YLIVPCILEA 490 500 510 520 530 540 HQKSEFVLRV FSRKHIFYEI GSNSGVVFSK EIEDQNERQD EFFTKFFEKH PEINAVQLQN 550 560 570 580 590 600 LLNQMTWSSL GSRQPFFSLE ACQGILALLD LNASGTMSIQ EFRDLWKQLK LSQKVFHKQD 610 620 630 640 650 660 RGSGYLNWEQ LHAAMREAGI MLSDDVCQLM LIRYGGPRLQ MDFVSFIHLM LRVENMEDVF 670 680 QNLTQDGKGI YLQKPEWMMM ALYS
	Isoform 2 [UniParc]. Checksum: F8DBD839E18AC7F7	FASTA	508	59,025
	10 20 30 40 50 60 MLASSGSGLS GSYEDLQSGQ VSEALVDFTG GVTMTINLAE AHGNLWDILI EATYNRTLIG 70 80 90 100 110 120 CQTHSGEKIL ENGLVEGHAY TLTGIRKVTC KHRPEYLVKL RNPWGKVEWK GDWSDSSSKW 130 140 150 160 170 180 ELLSPKEKIL LLRKDNDGEF WMTLQDFKTH FVLLVICKLT PGLLSQEAAQ KWTYTMREGR 190 200 210 220 230 240 WEKRSTAGGQ RQLLQDTFWK NPQFLLSVWR PEEGRRSLRP CSVLVSLLQK PRHRCRKRKP 250 260 270 280 290 300 LLAIGFYLYR MNKYHDDQRR LPPEFFQRNT PLSQPDRFLK EKEVSQELCL EPGTYLIVPC 310 320 330 340 350 360 ILEAHQKSEF VLRVFSRKHI FYEIGSNSGV VFSKEIEDQN ERQDEFFTKF FEKHPEINAV 370 380 390 400 410 420 QLQNLLNQMT WSSLGSRQPF FSLEACQGIL ALLDLNASGT MSIQEFRDLW KQLKLSQKVF 430 440 450 460 470 480 HKQDRGSGYL NWEQLHAAMR EAGIMLSDDV CQLMLIRYGG PRLQMDFVSF IHLMLRVENM 490 500 EDVFQNLTQD GKGIYLQKPE WMMMALYS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Teratocarcinoma.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Identification and characterization of two novel calpain large subunit genes." Dear T.N., Boehm T. Gene 274:245-252(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, LACK OF TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK092257 mRNA. Translation: BAG52511.1. AC015980 Genomic DNA. No translation available.
IPI	IPI00455530. IPI00895873.
RefSeq	NP_001138594.1. NM_001145122.1.
UniGene	Hs.468059.
3D structure databases
ProteinModelPortal	A8MX76.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000385247.
PTM databases
PhosphoSite	A8MX76.
Proteomic databases
PaxDb	A8MX76.
PRIDE	A8MX76.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000403897; ENSP00000385247; ENSG00000214711.
GeneID	440854.
KEGG	hsa:440854.
UCSC	uc002rnt.2. human. uc010yms.2. human.
Organism-specific databases
CTD	440854.
GeneCards	GC02M031370.
H-InvDB	HIX0001949.
HGNC	HGNC:16664. CAPN14.
HPA	HPA035721.
MIM	610229. gene.
neXtProt	NX_A8MX76.
PharmGKB	PA134888839.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG249866.
HOGENOM	HOG000232035.
HOVERGEN	HBG012645.
KO	K13442.
OMA	RVFSRKH.
OrthoDB	EOG4ZGPBR.
Gene expression databases
ArrayExpress	A8MX76.
Bgee	A8MX76.
CleanEx	HS_CAPN14.
Genevestigator	A8MX76.
Family and domain databases
Gene3D	1.10.238.10. 1 hit.
InterPro	IPR022684. Calpain_cysteine_protease. IPR022682. Calpain_domain_III. IPR022683. Calpain_III. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR000169. Pept_cys_AS. IPR001300. Peptidase_C2_calpain_cat. [Graphical view]
Pfam	PF01067. Calpain_III. 1 hit. PF00648. Peptidase_C2. 1 hit. [Graphical view]
PRINTS	PR00704. CALPAIN.
SMART	SM00720. calpain_III. 1 hit. SM00230. CysPc. 1 hit. [Graphical view]
SUPFAM	SSF49758. Peptidase_C2. 1 hit.
PROSITE	PS50203. CALPAIN_CAT. 1 hit. PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 3 hits. PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	109543.
SOURCE	Search...

Entry information

Entry name

CAN14_HUMAN

Accession

Primary (citable) accession number: A8MX76
Secondary accession number(s): B3KRU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	May 1, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.