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Protein

Calpain-14

Gene

CAPN14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei101 – 1011By similarity
Active sitei254 – 2541By similarity
Active sitei278 – 2781By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi570 – 58112PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: GO_Central
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. proteolysis Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.021.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-14 (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase 14
Short name:
CANP 14
Gene namesi
Name:CAPN14
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:16664. CAPN14.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. nucleoplasm Source: HPA
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134888839.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 684684Calpain-14PRO_0000341373Add
BLAST

Proteomic databases

MaxQBiA8MX76.
PaxDbiA8MX76.
PRIDEiA8MX76.

PTM databases

PhosphoSiteiA8MX76.

Expressioni

Tissue specificityi

Not expressed in tissues tested.

Gene expression databases

BgeeiA8MX76.
CleanExiHS_CAPN14.
ExpressionAtlasiA8MX76. baseline and differential.
GenevestigatoriA8MX76.

Organism-specific databases

HPAiHPA035720.
HPA035721.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000385247.

Structurei

3D structure databases

ProteinModelPortaliA8MX76.
SMRiA8MX76. Positions 28-682.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 336294Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini557 – 59236EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini586 – 62136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini651 – 68434EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni337 – 503167Domain IIIAdd
BLAST
Regioni504 – 51714LinkerAdd
BLAST
Regioni518 – 683166Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG249866.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiA8MX76.
KOiK13442.
OrthoDBiEOG7RV9FM.
PhylomeDBiA8MX76.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A8MX76-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLWPPFRCR WKLAPRYSRR ASPQQPQQDF EALLAECLRN GCLFEDTSFP
60 70 80 90 100
ATLSSIGSGS LLQKLPPRLQ WKRPPELHSN PQFYFAKAKR LDLCQGIVGD
110 120 130 140 150
CWFLAALQAL ALHQDILSRV VPLNQSFTEK YAGIFRFWFW HYGNWVPVVI
160 170 180 190 200
DDRLPVNEAG QLVFVSSTYK NLFWGALLEK AYAKLSGSYE DLQSGQVSEA
210 220 230 240 250
LVDFTGGVTM TINLAEAHGN LWDILIEATY NRTLIGCQTH SGEKILENGL
260 270 280 290 300
VEGHAYTLTG IRKVTCKHRP EYLVKLRNPW GKVEWKGDWS DSSSKWELLS
310 320 330 340 350
PKEKILLLRK DNDGEFWMTL QDFKTHFVLL VICKLTPGLL SQEAAQKWTY
360 370 380 390 400
TMREGRWEKR STAGGQRQLL QDTFWKNPQF LLSVWRPEEG RRSLRPCSVL
410 420 430 440 450
VSLLQKPRHR CRKRKPLLAI GFYLYRMNKY HDDQRRLPPE FFQRNTPLSQ
460 470 480 490 500
PDRFLKEKEV SQELCLEPGT YLIVPCILEA HQKSEFVLRV FSRKHIFYEI
510 520 530 540 550
GSNSGVVFSK EIEDQNERQD EFFTKFFEKH PEINAVQLQN LLNQMTWSSL
560 570 580 590 600
GSRQPFFSLE ACQGILALLD LNASGTMSIQ EFRDLWKQLK LSQKVFHKQD
610 620 630 640 650
RGSGYLNWEQ LHAAMREAGI MLSDDVCQLM LIRYGGPRLQ MDFVSFIHLM
660 670 680
LRVENMEDVF QNLTQDGKGI YLQKPEWMMM ALYS
Length:684
Mass (Da):79,568
Last modified:June 10, 2008 - v2
Checksum:i6627B859E4F375A9
GO
Isoform 2 (identifier: A8MX76-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-176: Missing.
     177-184: LLEKAYAK → MLASSGSG

Show »
Length:508
Mass (Da):59,025
Checksum:iF8DBD839E18AC7F7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 176176Missing in isoform 2. 1 PublicationVSP_034268Add
BLAST
Alternative sequencei177 – 1848LLEKAYAK → MLASSGSG in isoform 2. 1 PublicationVSP_034269

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK092257 mRNA. Translation: BAG52511.1.
AC015980 Genomic DNA. No translation available.
CCDSiCCDS46254.1. [A8MX76-1]
RefSeqiNP_001138594.1. NM_001145122.1. [A8MX76-1]
XP_006712085.1. XM_006712022.1. [A8MX76-2]
UniGeneiHs.468059.

Genome annotation databases

EnsembliENST00000403897; ENSP00000385247; ENSG00000214711. [A8MX76-1]
GeneIDi440854.
KEGGihsa:440854.
UCSCiuc002rnt.2. human. [A8MX76-2]
uc010yms.2. human. [A8MX76-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK092257 mRNA. Translation: BAG52511.1.
AC015980 Genomic DNA. No translation available.
CCDSiCCDS46254.1. [A8MX76-1]
RefSeqiNP_001138594.1. NM_001145122.1. [A8MX76-1]
XP_006712085.1. XM_006712022.1. [A8MX76-2]
UniGeneiHs.468059.

3D structure databases

ProteinModelPortaliA8MX76.
SMRiA8MX76. Positions 28-682.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000385247.

Protein family/group databases

MEROPSiC02.021.

PTM databases

PhosphoSiteiA8MX76.

Proteomic databases

MaxQBiA8MX76.
PaxDbiA8MX76.
PRIDEiA8MX76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000403897; ENSP00000385247; ENSG00000214711. [A8MX76-1]
GeneIDi440854.
KEGGihsa:440854.
UCSCiuc002rnt.2. human. [A8MX76-2]
uc010yms.2. human. [A8MX76-1]

Organism-specific databases

CTDi440854.
GeneCardsiGC02M031370.
H-InvDBHIX0001949.
HGNCiHGNC:16664. CAPN14.
HPAiHPA035720.
HPA035721.
MIMi610229. gene.
neXtProtiNX_A8MX76.
PharmGKBiPA134888839.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG249866.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiA8MX76.
KOiK13442.
OrthoDBiEOG7RV9FM.
PhylomeDBiA8MX76.
TreeFamiTF314748.

Miscellaneous databases

ChiTaRSiCAPN14. human.
NextBioi109543.
PROiA8MX76.
SOURCEiSearch...

Gene expression databases

BgeeiA8MX76.
CleanExiHS_CAPN14.
ExpressionAtlasiA8MX76. baseline and differential.
GenevestigatoriA8MX76.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Teratocarcinoma.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Identification and characterization of two novel calpain large subunit genes."
    Dear T.N., Boehm T.
    Gene 274:245-252(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, LACK OF TISSUE SPECIFICITY.

Entry informationi

Entry nameiCAN14_HUMAN
AccessioniPrimary (citable) accession number: A8MX76
Secondary accession number(s): B3KRU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: February 4, 2015
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.