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Protein

HEPACAM family member 2

Gene

HEPACAM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required during prometaphase for centrosome maturation. Following poly-ADP-ribosylation (PARsylation) by TNKS, translocates from the Golgi apparatus to mitotic centrosomes and plays a key role in the formation of robust microtubules for prompt movement of chromosomes: anchors AKAP9/CG-NAP, a scaffold protein of the gamma-tubulin ring complex and promotes centrosome maturation.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centrosome organization Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciZFISH:G66-31444-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HEPACAM family member 2
Alternative name(s):
Mitotic kinetics regulator
Gene namesi
Name:HEPACAM2
Synonyms:MIKI
ORF Names:UNQ305/PRO346
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:27364. HEPACAM2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei352 – 372HelicalSequence analysisAdd BLAST21
Topological domaini373 – 462CytoplasmicSequence analysisAdd BLAST90

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • midbody Source: UniProtKB
  • spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000188175.
PharmGKBiPA162390849.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000033222032 – 462HEPACAM family member 2Add BLAST431

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi85N-linked (GlcNAc...)Sequence analysis1
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Glycosylationi165N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi170 ↔ 219PROSITE-ProRule annotation
Disulfide bondi270 ↔ 315PROSITE-ProRule annotation
Glycosylationi320N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Poly-ADP-ribosylated (PARsylated) by tankyrase TNKS during late G2 and prophase, leading to translocation to mitotic centrosomes.
N-glycosylated.2 Publications

Keywords - PTMi

ADP-ribosylation, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiA8MVW5.
PRIDEiA8MVW5.

PTM databases

iPTMnetiA8MVW5.
PhosphoSitePlusiA8MVW5.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000188175.
CleanExiHS_HEPACAM2.
ExpressionAtlasiA8MVW5. baseline and differential.
GenevisibleiA8MVW5. HS.

Interactioni

Protein-protein interaction databases

BioGridi128949. 37 interactors.
STRINGi9606.ENSP00000377980.

Structurei

3D structure databases

ProteinModelPortaliA8MVW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 233Ig-like C2-type 1Add BLAST85
Domaini235 – 331Ig-like C2-type 2Add BLAST97

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IK28. Eukaryota.
ENOG410Z756. LUCA.
GeneTreeiENSGT00530000063550.
HOGENOMiHOG000263412.
HOVERGENiHBG063553.
InParanoidiA8MVW5.
OMAiIYYGPYG.
OrthoDBiEOG091G05OZ.
PhylomeDBiA8MVW5.
TreeFamiTF331199.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
SM00408. IGc2. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A8MVW5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQDAFMEPF GDTLGVFQCK IYLLLFGACS GLKVTVPSHT VHGVRGQALY
60 70 80 90 100
LPVHYGFHTP ASDIQIIWLF ERPHTMPKYL LGSVNKSVVP DLEYQHKFTM
110 120 130 140 150
MPPNASLLIN PLQFPDEGNY IVKVNIQGNG TLSASQKIQV TVDDPVTKPV
160 170 180 190 200
VQIHPPSGAV EYVGNMTLTC HVEGGTRLAY QWLKNGRPVH TSSTYSFSPQ
210 220 230 240 250
NNTLHIAPVT KEDIGNYSCL VRNPVSEMES DIIMPIIYYG PYGLQVNSDK
260 270 280 290 300
GLKVGEVFTV DLGEAILFDC SADSHPPNTY SWIRRTDNTT YIIKHGPRLE
310 320 330 340 350
VASEKVAQKT MDYVCCAYNN ITGRQDETHF TVIITSVGLE KLAQKGKSLS
360 370 380 390 400
PLASITGISL FLIISMCLLF LWKKYQPYKV IKQKLEGRPE TEYRKAQTFS
410 420 430 440 450
GHEDALDDFG IYEFVAFPDV SGVSRIPSRS VPASDCVSGQ DLHSTVYEVI
460
QHIPAQQQDH PE
Length:462
Mass (Da):51,407
Last modified:December 4, 2007 - v1
Checksum:iE38DE6E4CCAA6BC2
GO
Isoform 2 (identifier: A8MVW5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MGQDAFMEPFGDTLGVFQCKIYLLLF → MWLKVFTTFLSFAT

Show »
Length:450
Mass (Da):50,114
Checksum:iA22FF822CC3CB226
GO
Isoform 3 (identifier: A8MVW5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     26-26: F → FDIVANCLLLRFKLSVSSYEIWKK

Note: No experimental confirmation available.
Show »
Length:485
Mass (Da):54,115
Checksum:iAEEBBDD00466C282
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti277P → S in BAG60603 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04297631G → R in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_04297786K → T.Corresponds to variant rs10281525dbSNPEnsembl.1
Natural variantiVAR_064721114F → L Found in a renal cell carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant rs555587403dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0333551 – 26MGQDA…YLLLF → MWLKVFTTFLSFAT in isoform 2. 2 PublicationsAdd BLAST26
Alternative sequenceiVSP_04432226F → FDIVANCLLLRFKLSVSSYE IWKK in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358345 mRNA. Translation: AAQ88711.1.
AK096002 mRNA. Translation: BAG53196.1.
AK298361 mRNA. Translation: BAG60603.1.
AC000119 Genomic DNA. No translation available.
AC002453 Genomic DNA. No translation available.
BC136300 mRNA. Translation: AAI36301.1.
BC136301 mRNA. Translation: AAI36302.1.
BC139906 mRNA. Translation: AAI39907.1.
CCDSiCCDS43616.1. [A8MVW5-1]
CCDS5629.1. [A8MVW5-2]
CCDS75632.1. [A8MVW5-3]
RefSeqiNP_001034461.1. NM_001039372.2. [A8MVW5-1]
NP_001275733.1. NM_001288804.1. [A8MVW5-3]
NP_001275739.1. NM_001288810.1.
NP_937794.1. NM_198151.2. [A8MVW5-2]
UniGeneiHs.443169.

Genome annotation databases

EnsembliENST00000341723; ENSP00000340532; ENSG00000188175. [A8MVW5-2]
ENST00000394468; ENSP00000377980; ENSG00000188175. [A8MVW5-1]
ENST00000453812; ENSP00000390204; ENSG00000188175. [A8MVW5-3]
GeneIDi253012.
KEGGihsa:253012.
UCSCiuc003uml.5. human. [A8MVW5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY358345 mRNA. Translation: AAQ88711.1.
AK096002 mRNA. Translation: BAG53196.1.
AK298361 mRNA. Translation: BAG60603.1.
AC000119 Genomic DNA. No translation available.
AC002453 Genomic DNA. No translation available.
BC136300 mRNA. Translation: AAI36301.1.
BC136301 mRNA. Translation: AAI36302.1.
BC139906 mRNA. Translation: AAI39907.1.
CCDSiCCDS43616.1. [A8MVW5-1]
CCDS5629.1. [A8MVW5-2]
CCDS75632.1. [A8MVW5-3]
RefSeqiNP_001034461.1. NM_001039372.2. [A8MVW5-1]
NP_001275733.1. NM_001288804.1. [A8MVW5-3]
NP_001275739.1. NM_001288810.1.
NP_937794.1. NM_198151.2. [A8MVW5-2]
UniGeneiHs.443169.

3D structure databases

ProteinModelPortaliA8MVW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128949. 37 interactors.
STRINGi9606.ENSP00000377980.

PTM databases

iPTMnetiA8MVW5.
PhosphoSitePlusiA8MVW5.

Proteomic databases

PaxDbiA8MVW5.
PRIDEiA8MVW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000341723; ENSP00000340532; ENSG00000188175. [A8MVW5-2]
ENST00000394468; ENSP00000377980; ENSG00000188175. [A8MVW5-1]
ENST00000453812; ENSP00000390204; ENSG00000188175. [A8MVW5-3]
GeneIDi253012.
KEGGihsa:253012.
UCSCiuc003uml.5. human. [A8MVW5-1]

Organism-specific databases

CTDi253012.
GeneCardsiHEPACAM2.
HGNCiHGNC:27364. HEPACAM2.
MIMi614133. gene.
neXtProtiNX_A8MVW5.
OpenTargetsiENSG00000188175.
PharmGKBiPA162390849.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IK28. Eukaryota.
ENOG410Z756. LUCA.
GeneTreeiENSGT00530000063550.
HOGENOMiHOG000263412.
HOVERGENiHBG063553.
InParanoidiA8MVW5.
OMAiIYYGPYG.
OrthoDBiEOG091G05OZ.
PhylomeDBiA8MVW5.
TreeFamiTF331199.

Enzyme and pathway databases

BioCyciZFISH:G66-31444-MONOMER.

Miscellaneous databases

GenomeRNAii253012.
PROiA8MVW5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000188175.
CleanExiHS_HEPACAM2.
ExpressionAtlasiA8MVW5. baseline and differential.
GenevisibleiA8MVW5. HS.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
SM00408. IGc2. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHECA2_HUMAN
AccessioniPrimary (citable) accession number: A8MVW5
Secondary accession number(s): B3KTT4
, B4DPJ1, B9EG93, E9PDV5, Q6UXI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: December 4, 2007
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.