A8MU46 (SMTL1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Smoothelin-like protein 1

Gene names

Name:

SMTNL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the regulation of contractile properties of both striated and smooth muscles. When unphosphorylated, may inhibit myosin dephosphorylation. Phosphorylation at Ser-299 reduces this inhibitory activity By similarity.
Subunit structure	Interacts with PPP1R12A By similarity.
Subcellular location	Cytoplasm › myofibril. Cytoplasm › myofibril › sarcomere › I band By similarity. Cytoplasm › myofibril › sarcomere › M line By similarity. Nucleus By similarity. Note: Colocalizes with MYH2. In its unphosphorylated state, localizes to the cytoplasm By similarity. Phosphorylation at Ser-301 promotes translocation to the nucleus By similarity. Ref.2
Tissue specificity	Expressed in striated muscles, specifically in type 2a fibers (at protein level). Ref.2 Ref.3
Post-translational modification	Maximal phosphorylation of Ser-299 correlates with maximal relaxation of aorta in response to acetylcholine By similarity.
Sequence similarities	Belongs to the smoothelin family. Contains 1 CH (calponin-homology) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil
Ligand	Calmodulin-binding
Molecular function	Muscle protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of vasodilation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of vasoconstriction Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	I band Inferred from electronic annotation. Source: UniProtKB-SubCell M band Inferred from electronic annotation. Source: UniProtKB-SubCell contractile fiber Inferred from direct assay Ref.3. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Smoothelin-like protein 1		PRO_0000317275
Regions
	Domain	341 – 444	104	CH
	Region	439 – 457	19	Calmodulin-binding By similarity
	Coiled coil	123 – 145	23	Potential
	Compositional bias	44 – 245	202	Glu-rich
Amino acid modifications
	Modified residue	299	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MU46 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 54C5DC43EECC3C66	FASTA	457	48,953
	10 20 30 40 50 60 MEQKEGKLSE DGTTVSPAAD NPEMSGGGAP AEETKGTAGK AINEGPPTES GKQEKAPAED 70 80 90 100 110 120 GMSAELQGEA NGLDEVKVES QREAGGKEDA EAELKKEDGE KEETTVGSQE MTGRKEETKS 130 140 150 160 170 180 EPKEAEEKES TLASEKQKAE EKEAKPESGQ KADANDRDKP EPKATVEEED AKTASQEETV 190 200 210 220 230 240 VEDEAKAEPK EPDGKEEAKH GAKEEADEPG SPSEEQEQDV EKEPEGGAGV IPSSPEEWPE 250 260 270 280 290 300 SPTGEGHNLS TDGLGPDCVA SGQTSPSASE SSPSDVPQSP PESPSSGEKK EKAPERRVSA 310 320 330 340 350 360 PARPRGPRAQ NRKAIVDKFG GAASGPTALF RNTKAAGAAI GGVKNMLLEW CRAMTKKYEH 370 380 390 400 410 420 VDIQNFSSSW SSGMAFCALI HKFFPDAFDY AELDPAKRRH NFTLAFSTAE KLADCAQLLD 430 440 450 VDDMVRLAVP DSKCVYTYIQ ELYRSLVQKG LVKTKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Deletion of the protein kinase A/protein kinase G target SMTNL1 promotes an exercise-adapted phenotype in vascular smooth muscle." Wooldridge A.A., Fortner C.N., Lontay B., Akimoto T., Neppl R.L., Facemire C., Datto M.B., Kwon A., McCook E., Li P., Wang S., Thresher R.J., Miller S.E., Perriard J.C., Gavin T.P., Hickner R.C., Coffman T.M., Somlyo A.V., Yan Z., Haystead T.A. J. Biol. Chem. 283:11850-11859(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"Smoothelin-like 1 protein regulates myosin phosphatase-targeting subunit 1 expression during sexual development and pregnancy." Lontay B., Bodoor K., Weitzel D.H., Loiselle D., Fortner C., Lengyel S., Zheng D., Devente J., Hickner R., Haystead T.A. J. Biol. Chem. 285:29357-29366(2010) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP002893 Genomic DNA. No translation available.
IPI	IPI00377167.
UniGene	Hs.68756.
3D structure databases
ProteinModelPortal	A8MU46.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000406485.
Proteomic databases
PaxDb	A8MU46.
PRIDE	A8MU46.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000399154; ENSP00000382108; ENSG00000214872.
Organism-specific databases
GeneCards	GC11P057309.
HGNC	HGNC:32394. SMTNL1.
HPA	HPA040264.
MIM	613664. gene.
neXtProt	NX_A8MU46.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5069.
HOGENOM	HOG000154352.
HOVERGEN	HBG095368.
InParanoid	A8MU46.
Gene expression databases
ArrayExpress	A8MU46.
Bgee	A8MU46.
CleanEx	HS_SMTNL1.
Genevestigator	A8MU46.
Family and domain databases
Gene3D	1.10.418.10. 1 hit.
InterPro	IPR001715. CH-domain. [Graphical view]
Pfam	PF00307. CH. 1 hit. [Graphical view]
SMART	SM00033. CH. 1 hit. [Graphical view]
SUPFAM	SSF47576. Calponin-homology. 1 hit.
PROSITE	PS50021. CH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

SMTL1_HUMAN

Accession

Primary (citable) accession number: A8MU46

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.