Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A8MTJ3

- GNAT3_HUMAN

UniProt

A8MTJ3 - GNAT3_HUMAN

Protein

Guanine nucleotide-binding protein G(t) subunit alpha-3

Gene

GNAT3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 2 (01 Jul 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471MagnesiumBy similarity
    Metal bindingi181 – 1811MagnesiumBy similarity
    Binding sitei326 – 3261GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi40 – 478GTPBy similarity
    Nucleotide bindingi175 – 1817GTPBy similarity
    Nucleotide bindingi200 – 2045GTPBy similarity
    Nucleotide bindingi269 – 2724GTPBy similarity

    GO - Molecular functioni

    1. G-protein beta/gamma-subunit complex binding Source: RefGenome
    2. G-protein coupled photoreceptor activity Source: RefGenome
    3. G-protein coupled receptor binding Source: RefGenome
    4. GTPase activity Source: RefGenome
    5. GTP binding Source: UniProtKB-KW
    6. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: Reactome
    2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
    3. blood coagulation Source: Reactome
    4. detection of chemical stimulus involved in sensory perception of bitter taste Source: RefGenome
    5. detection of visible light Source: GOC
    6. platelet activation Source: Reactome
    7. response to nicotine Source: Ensembl
    8. sensory perception of sweet taste Source: RefGenome
    9. sensory perception of umami taste Source: Ensembl
    10. synaptic transmission Source: Reactome

    Keywords - Molecular functioni

    Transducer

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_15333. Adenylate cyclase inhibitory pathway.
    REACT_15426. PLC beta mediated events.
    REACT_15457. G-protein activation.
    REACT_19231. G alpha (i) signalling events.
    REACT_19327. G alpha (s) signalling events.
    REACT_19333. G alpha (z) signalling events.
    REACT_20653. ADP signalling through P2Y purinoceptor 12.
    REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein G(t) subunit alpha-3
    Alternative name(s):
    Gustducin alpha-3 chain
    Gene namesi
    Name:GNAT3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:22800. GNAT3.

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: Dual ditribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm.

    GO - Cellular componenti

    1. acrosomal vesicle Source: Ensembl
    2. apical plasma membrane Source: Ensembl
    3. axoneme Source: Ensembl
    4. heterotrimeric G-protein complex Source: RefGenome
    5. photoreceptor inner segment Source: RefGenome
    6. photoreceptor outer segment Source: RefGenome
    7. plasma membrane Source: Reactome
    8. protein complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134931156.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 354353Guanine nucleotide-binding protein G(t) subunit alpha-3PRO_0000342671Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity

    Post-translational modificationi

    Potential N-myristoylation may anchor alpha-subunit to the inner surface of plasma membrane.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate

    Proteomic databases

    MaxQBiA8MTJ3.
    PaxDbiA8MTJ3.
    PRIDEiA8MTJ3.

    PTM databases

    PhosphoSiteiA8MTJ3.

    Expressioni

    Tissue specificityi

    Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and foliate papillae of the tongue at protein level. Expressed in enteroendocrine L cells of the gut. Detected also in spermatozoa.4 Publications

    Gene expression databases

    BgeeiA8MTJ3.
    CleanExiHS_GNAT3.
    GenevestigatoriA8MTJ3.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma, respectively GNAT3, GNB1 and GNG13 for Gustducin heterotrimer for bitter taste transduction. The alpha chain contains the guanine nucleotide binding site. Gustducin heterotrimer may also be composed of GNAT3, GNB3 and GNG13.

    Protein-protein interaction databases

    BioGridi131390. 4 interactions.
    IntActiA8MTJ3. 1 interaction.
    STRINGi9606.ENSP00000381339.

    Structurei

    3D structure databases

    ProteinModelPortaliA8MTJ3.
    SMRiA8MTJ3. Positions 14-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG322962.
    HOGENOMiHOG000038730.
    HOVERGENiHBG063184.
    InParanoidiA8MTJ3.
    KOiK04631.
    OMAiKVHLSVC.
    OrthoDBiEOG72C50B.
    PhylomeDBiA8MTJ3.
    TreeFamiTF300673.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A8MTJ3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSGISSESK ESAKRSKELE KKLQEDAERD ARTVKLLLLG AGESGKSTIV    50
    KQMKIIHKNG YSEQECMEFK AVIYSNTLQS ILAIVKAMTT LGIDYVNPRS 100
    AEDQRQLYAM ANTLEDGGMT PQLAEVIKRL WRDPGIQACF ERASEYQLND 150
    SAAYYLNDLD RITASGYVPN EQDVLHSRVK TTGIIETQFS FKDLHFRMFD 200
    VGGQRSERKK WIHCFEGVTC IIFCAALSAY DMVLVEDEEV NRMHESLHLF 250
    NSICNHKYFS TTSIVLFLNK KDIFQEKVTK VHLSICFPEY TGPNTFEDAG 300
    NYIKNQFLDL NLKKEDKEIY SHMTCATDTQ NVKFVFDAVT DIIIKENLKD 350
    CGLF 354
    Length:354
    Mass (Da):40,357
    Last modified:July 1, 2008 - v2
    Checksum:i47366FB99E9322AD
    GO

    Sequence cautioni

    The sequence EAL24192.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence EAL24193.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004862 Genomic DNA. No translation available.
    CH236949 Genomic DNA. Translation: EAL24192.1. Sequence problems.
    CH236949 Genomic DNA. Translation: EAL24193.1. Sequence problems.
    CH471091 Genomic DNA. Translation: EAW77007.1.
    BC147016 mRNA. Translation: AAI47017.1.
    BC147017 mRNA. Translation: AAI47018.1.
    CCDSiCCDS47625.1.
    RefSeqiNP_001095856.1. NM_001102386.1.
    UniGeneiHs.335049.

    Genome annotation databases

    EnsembliENST00000398291; ENSP00000381339; ENSG00000214415.
    GeneIDi346562.
    KEGGihsa:346562.
    UCSCiuc011kgu.2. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004862 Genomic DNA. No translation available.
    CH236949 Genomic DNA. Translation: EAL24192.1 . Sequence problems.
    CH236949 Genomic DNA. Translation: EAL24193.1 . Sequence problems.
    CH471091 Genomic DNA. Translation: EAW77007.1 .
    BC147016 mRNA. Translation: AAI47017.1 .
    BC147017 mRNA. Translation: AAI47018.1 .
    CCDSi CCDS47625.1.
    RefSeqi NP_001095856.1. NM_001102386.1.
    UniGenei Hs.335049.

    3D structure databases

    ProteinModelPortali A8MTJ3.
    SMRi A8MTJ3. Positions 14-354.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 131390. 4 interactions.
    IntActi A8MTJ3. 1 interaction.
    STRINGi 9606.ENSP00000381339.

    PTM databases

    PhosphoSitei A8MTJ3.

    Proteomic databases

    MaxQBi A8MTJ3.
    PaxDbi A8MTJ3.
    PRIDEi A8MTJ3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000398291 ; ENSP00000381339 ; ENSG00000214415 .
    GeneIDi 346562.
    KEGGi hsa:346562.
    UCSCi uc011kgu.2. human.

    Organism-specific databases

    CTDi 346562.
    GeneCardsi GC07M080087.
    HGNCi HGNC:22800. GNAT3.
    MIMi 139395. gene.
    neXtProti NX_A8MTJ3.
    PharmGKBi PA134931156.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG322962.
    HOGENOMi HOG000038730.
    HOVERGENi HBG063184.
    InParanoidi A8MTJ3.
    KOi K04631.
    OMAi KVHLSVC.
    OrthoDBi EOG72C50B.
    PhylomeDBi A8MTJ3.
    TreeFami TF300673.

    Enzyme and pathway databases

    Reactomei REACT_15333. Adenylate cyclase inhibitory pathway.
    REACT_15426. PLC beta mediated events.
    REACT_15457. G-protein activation.
    REACT_19231. G alpha (i) signalling events.
    REACT_19327. G alpha (s) signalling events.
    REACT_19333. G alpha (z) signalling events.
    REACT_20653. ADP signalling through P2Y purinoceptor 12.
    REACT_24019. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).

    Miscellaneous databases

    GeneWikii GNAT3.
    GenomeRNAii 346562.
    NextBioi 98979.
    PROi A8MTJ3.
    SOURCEi Search...

    Gene expression databases

    Bgeei A8MTJ3.
    CleanExi HS_GNAT3.
    Genevestigatori A8MTJ3.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001408. Gprotein_alpha_I.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00441. GPROTEINAI.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Human chromosome 7: DNA sequence and biology."
      Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
      , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
      Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Human taste cells express the G protein alpha-gustducin and neuron-specific enolase."
      Takami S., Getchell T.V., McLaughlin S.K., Margolskee R.F., Getchell M.L.
      Brain Res. Mol. Brain Res. 22:193-203(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    6. Cited for: FUNCTION.
    7. "Colocalization of the alpha-subunit of gustducin with PYY and GLP-1 in L cells of human colon."
      Rozengurt N., Wu S.V., Chen M.C., Huang C., Sternini C., Rozengurt E.
      Am. J. Physiol. 291:G792-G802(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    8. "Expression of the G-protein alpha-subunit gustducin in mammalian spermatozoa."
      Fehr J., Meyer D., Widmayer P., Borth H.C., Ackermann F., Wilhelm B., Gudermann T., Boekhoff I.
      J. Comp. Physiol. A 193:21-34(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    9. Cited for: FUNCTION.
    10. Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGNAT3_HUMAN
    AccessioniPrimary (citable) accession number: A8MTJ3
    Secondary accession number(s): A4D1B2, A4D1B3, B9EJG5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 67 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3