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A8MT69

- CENPX_HUMAN

UniProt

A8MT69 - CENPX_HUMAN

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Protein

Centromere protein X

Gene
STRA13, CENPX, FAAP10, MHF2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation.3 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. protein binding Source: UniProtKB

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. kinetochore assembly Source: InterPro
  3. mitotic nuclear division Source: UniProtKB-KW
  4. positive regulation of protein ubiquitination Source: UniProtKB
  5. replication fork processing Source: UniProtKB
  6. resolution of meiotic recombination intermediates Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA repair, Mitosis

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein X
Short name:
CENP-X
Alternative name(s):
FANCM-interacting histone fold protein 2
Fanconi anemia-associated polypeptide of 10 kDa
Retinoic acid-inducible gene D9 protein homolog
Stimulated by retinoic acid gene 13 protein homolog
Gene namesi
Name:STRA13
Synonyms:CENPX, FAAP10, MHF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:11422. STRA13.

Subcellular locationi

Nucleus. Chromosomecentromere. Chromosomecentromerekinetochore
Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis.2 Publications

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB-SubCell
  2. FANCM-MHF complex Source: UniProtKB
  3. Fanconi anaemia nuclear complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Fanconi anemia

Organism-specific databases

PharmGKBiPA36223.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8181Centromere protein XPRO_0000337180Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiA8MT69.
PaxDbiA8MT69.
PRIDEiA8MT69.

Expressioni

Gene expression databases

ArrayExpressiA8MT69.
BgeeiA8MT69.
CleanExiHS_STRA13.
GenevestigatoriA8MT69.

Organism-specific databases

HPAiHPA027348.

Interactioni

Subunit structurei

Belongs to the multisubunit FA complex composed of APITD1/CENPS, FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, FANCM, FAAP24 and STRA13/CENPX. Interacts with APITD1/CENPS, FANCM and FAAP24. Component of a discrete APITD1/CENPS complex composed of at least APITD1/CENPS and STRA13/CENPX; this complex binds DNA. The heterodimer composed of APITD1/CENPS and STRA13/CENPX can dimerize to form a heterotetramer. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APITD1Q8N2Z96EBI-5529694,EBI-5529649

Protein-protein interaction databases

BioGridi128376. 7 interactions.
IntActiA8MT69. 1 interaction.
STRINGi9606.ENSP00000302951.

Structurei

Secondary structure

1
81
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 209
Helixi32 – 5928
Beta strandi63 – 653
Helixi67 – 8014

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DRAX-ray2.41E/F/G/H1-81[»]
4DRBX-ray2.63J/K/L/M/N/O1-81[»]
4E44X-ray2.10B/D1-81[»]
4E45X-ray2.00B/D/G/I/L/N1-81[»]
4NDYX-ray7.00B/D/H/L/M/N/U/V/W/X8-81[»]
4NE1X-ray6.50B/D/H/L/M/N/U/V/W/X/Z/b/d/h/i/j/o/p/q/r8-81[»]
4NE3X-ray1.80B8-81[»]
4NE5X-ray2.50B/D/F/H8-81[»]
4NE6X-ray2.10B/D8-81[»]
ProteinModelPortaliA8MT69.
SMRiA8MT69. Positions 8-81.

Family & Domainsi

Sequence similaritiesi

Belongs to the CENPX family.

Phylogenomic databases

eggNOGiNOG71130.
HOGENOMiHOG000231242.
InParanoidiA8MT69.
KOiK15360.
PhylomeDBiA8MT69.

Family and domain databases

InterProiIPR018552. CENP-X.
[Graphical view]
PfamiPF09415. CENP-X. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A8MT69-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEGAGAGSGF RKELVSRLLH LHFKDDKTKV SGDALQLMVE LLKVFVVEAA   50
VRGVRQAQAE DALRVDVDQL EKVLPQLLLD F 81
Length:81
Mass (Da):8,959
Last modified:December 4, 2007 - v1
Checksum:i0BA47A4F0FFD2978
GO
Isoform 2 (identifier: A8MT69-2) [UniParc]FASTAAdd to Basket

Also known as: Variant A

The sequence of this isoform differs from the canonical sequence as follows:
     30-47: Missing.

Show »
Length:63
Mass (Da):7,017
Checksum:iB751CBF3D27E6B1A
GO
Isoform 3 (identifier: A8MT69-3) [UniParc]FASTAAdd to Basket

Also known as: Variant B

The sequence of this isoform differs from the canonical sequence as follows:
     30-47: Missing.
     73-77: Missing.

Show »
Length:58
Mass (Da):6,466
Checksum:i5166DA91818795E5
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei30 – 4718Missing in isoform 2 and isoform 3. VSP_033948Add
BLAST
Alternative sequencei73 – 775Missing in isoform 3. VSP_033949

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651V → A in AAH09571. 1 Publication
Sequence conflicti73 – 731V → L in AAB53638. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U95006 mRNA. Translation: AAB53638.1.
U95007 mRNA. Translation: AAB53639.1.
AC137723 Genomic DNA. No translation available.
BC009571 mRNA. Translation: AAH09571.1.
BC011610 mRNA. Translation: AAH11610.1.
CCDSiCCDS32772.1. [A8MT69-2]
CCDS59302.1. [A8MT69-3]
CCDS59303.1. [A8MT69-1]
RefSeqiNP_001257935.1. NM_001271006.1. [A8MT69-1]
NP_001257936.1. NM_001271007.1. [A8MT69-3]
NP_659435.2. NM_144998.3. [A8MT69-2]
UniGeneiHs.37616.

Genome annotation databases

EnsembliENST00000306704; ENSP00000302951; ENSG00000169689. [A8MT69-2]
ENST00000392359; ENSP00000376168; ENSG00000169689. [A8MT69-1]
ENST00000580435; ENSP00000462015; ENSG00000169689. [A8MT69-3]
GeneIDi201254.
KEGGihsa:201254.
UCSCiuc002kdc.4. human. [A8MT69-2]
uc002kdd.4. human. [A8MT69-3]
uc031rey.1. human. [A8MT69-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U95006 mRNA. Translation: AAB53638.1 .
U95007 mRNA. Translation: AAB53639.1 .
AC137723 Genomic DNA. No translation available.
BC009571 mRNA. Translation: AAH09571.1 .
BC011610 mRNA. Translation: AAH11610.1 .
CCDSi CCDS32772.1. [A8MT69-2 ]
CCDS59302.1. [A8MT69-3 ]
CCDS59303.1. [A8MT69-1 ]
RefSeqi NP_001257935.1. NM_001271006.1. [A8MT69-1 ]
NP_001257936.1. NM_001271007.1. [A8MT69-3 ]
NP_659435.2. NM_144998.3. [A8MT69-2 ]
UniGenei Hs.37616.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4DRA X-ray 2.41 E/F/G/H 1-81 [» ]
4DRB X-ray 2.63 J/K/L/M/N/O 1-81 [» ]
4E44 X-ray 2.10 B/D 1-81 [» ]
4E45 X-ray 2.00 B/D/G/I/L/N 1-81 [» ]
4NDY X-ray 7.00 B/D/H/L/M/N/U/V/W/X 8-81 [» ]
4NE1 X-ray 6.50 B/D/H/L/M/N/U/V/W/X/Z/b/d/h/i/j/o/p/q/r 8-81 [» ]
4NE3 X-ray 1.80 B 8-81 [» ]
4NE5 X-ray 2.50 B/D/F/H 8-81 [» ]
4NE6 X-ray 2.10 B/D 8-81 [» ]
ProteinModelPortali A8MT69.
SMRi A8MT69. Positions 8-81.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 128376. 7 interactions.
IntActi A8MT69. 1 interaction.
STRINGi 9606.ENSP00000302951.

Proteomic databases

MaxQBi A8MT69.
PaxDbi A8MT69.
PRIDEi A8MT69.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000306704 ; ENSP00000302951 ; ENSG00000169689 . [A8MT69-2 ]
ENST00000392359 ; ENSP00000376168 ; ENSG00000169689 . [A8MT69-1 ]
ENST00000580435 ; ENSP00000462015 ; ENSG00000169689 . [A8MT69-3 ]
GeneIDi 201254.
KEGGi hsa:201254.
UCSCi uc002kdc.4. human. [A8MT69-2 ]
uc002kdd.4. human. [A8MT69-3 ]
uc031rey.1. human. [A8MT69-1 ]

Organism-specific databases

CTDi 201254.
GeneCardsi GC17M079977.
H-InvDB HIX0014271.
HGNCi HGNC:11422. STRA13.
HPAi HPA027348.
neXtProti NX_A8MT69.
PharmGKBi PA36223.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG71130.
HOGENOMi HOG000231242.
InParanoidi A8MT69.
KOi K15360.
PhylomeDBi A8MT69.

Miscellaneous databases

GenomeRNAii 201254.
NextBioi 90103.
PROi A8MT69.

Gene expression databases

ArrayExpressi A8MT69.
Bgeei A8MT69.
CleanExi HS_STRA13.
Genevestigatori A8MT69.

Family and domain databases

InterProi IPR018552. CENP-X.
[Graphical view ]
Pfami PF09415. CENP-X. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of two human D9 transcripts."
    Scott L.M., Collins S.J.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
  2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-81 (ISOFORM 3).
    Tissue: Placenta and Skin.
  4. "The CENP-S complex is essential for the stable assembly of outer kinetochore structure."
    Amano M., Suzuki A., Hori T., Backer C., Okawa K., Cheeseman I.M., Fukagawa T.
    J. Cell Biol. 186:173-182(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH APITD1.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FA CORE COMPLEX, INTERACTION WITH FANCM AND APITD1, SUBCELLULAR LOCATION, DNA-BINDING, FUNCTION.
  6. "MHF1-MHF2, a histone-fold-containing protein complex, participates in the Fanconi anemia pathway via FANCM."
    Singh T.R., Saro D., Ali A.M., Zheng X.-F., Du C., Killen M.W., Sachpatzidis A., Wahengbam K., Pierce A.J., Xiong Y., Sung P., Meetei A.R.
    Mol. Cell 37:879-886(2010)
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FA CORE COMPLEX, INTERACTION WITH FANCM AND APITD1, SUBCELLULAR LOCATION, DNA-BINDING, FUNCTION.
  7. "CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."
    Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
    Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCENPX_HUMAN
AccessioniPrimary (citable) accession number: A8MT69
Secondary accession number(s): O00281
, O00282, Q96DD4, Q96F51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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