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Protein

Centromere protein X

Gene

STRA13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation.3 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • CENP-A containing nucleosome assembly Source: Reactome
  • DNA repair Source: UniProtKB-KW
  • kinetochore assembly Source: InterPro
  • mitotic nuclear division Source: UniProtKB-KW
  • nucleosome assembly Source: Reactome
  • positive regulation of protein ubiquitination Source: UniProtKB
  • replication fork processing Source: UniProtKB
  • resolution of meiotic recombination intermediates Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA repair, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein X
Short name:
CENP-X
Alternative name(s):
FANCM-interacting histone fold protein 2
Fanconi anemia-associated polypeptide of 10 kDa
Retinoic acid-inducible gene D9 protein homolog
Stimulated by retinoic acid gene 13 protein homolog
Gene namesi
Name:STRA13
Synonyms:CENPX, FAAP10, MHF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:11422. STRA13.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • FANCM-MHF complex Source: UniProtKB
  • Fanconi anaemia nuclear complex Source: UniProtKB
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Fanconi anemia

Organism-specific databases

PharmGKBiPA36223.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8181Centromere protein XPRO_0000337180Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiA8MT69.
PaxDbiA8MT69.
PRIDEiA8MT69.

Expressioni

Gene expression databases

BgeeiA8MT69.
CleanExiHS_STRA13.
ExpressionAtlasiA8MT69. baseline and differential.
GenevisibleiA8MT69. HS.

Organism-specific databases

HPAiHPA027348.

Interactioni

Subunit structurei

Belongs to the multisubunit FA complex composed of APITD1/CENPS, FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, FANCM, FAAP24 and STRA13/CENPX. Interacts with APITD1/CENPS, FANCM and FAAP24. Component of a discrete APITD1/CENPS complex composed of at least APITD1/CENPS and STRA13/CENPX; this complex binds DNA. The heterodimer composed of APITD1/CENPS and STRA13/CENPX can dimerize to form a heterotetramer. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APITD1Q8N2Z96EBI-5529694,EBI-5529649
RELQ048643EBI-5529694,EBI-307352
TRIM54Q9BYV23EBI-5529694,EBI-2130429

Protein-protein interaction databases

BioGridi128376. 18 interactions.
IntActiA8MT69. 3 interactions.

Structurei

Secondary structure

1
81
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 209Combined sources
Helixi32 – 5928Combined sources
Beta strandi63 – 653Combined sources
Helixi67 – 8014Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DRAX-ray2.41E/F/G/H1-81[»]
4DRBX-ray2.63J/K/L/M/N/O1-81[»]
4E44X-ray2.10B/D1-81[»]
4E45X-ray2.00B/D/G/I/L/N1-81[»]
4NDYX-ray7.00B/D/H/L/M/N/U/V/W/X8-81[»]
4NE1X-ray6.50B/D/H/L/M/N/U/V/W/X/Z/b/d/h/i/j/o/p/q/r8-81[»]
4NE3X-ray1.80B8-81[»]
4NE5X-ray2.50B/D/F/H8-81[»]
4NE6X-ray2.10B/D8-81[»]
ProteinModelPortaliA8MT69.
SMRiA8MT69. Positions 8-81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CENPX family.Curated

Phylogenomic databases

eggNOGiNOG71130.
GeneTreeiENSGT00390000002725.
HOGENOMiHOG000231242.
InParanoidiA8MT69.
KOiK15360.
PhylomeDBiA8MT69.

Family and domain databases

InterProiIPR018552. CENP-X.
[Graphical view]
PfamiPF09415. CENP-X. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A8MT69-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGAGAGSGF RKELVSRLLH LHFKDDKTKV SGDALQLMVE LLKVFVVEAA
60 70 80
VRGVRQAQAE DALRVDVDQL EKVLPQLLLD F
Length:81
Mass (Da):8,959
Last modified:December 4, 2007 - v1
Checksum:i0BA47A4F0FFD2978
GO
Isoform 2 (identifier: A8MT69-2) [UniParc]FASTAAdd to basket

Also known as: Variant A

The sequence of this isoform differs from the canonical sequence as follows:
     30-47: Missing.

Show »
Length:63
Mass (Da):7,017
Checksum:iB751CBF3D27E6B1A
GO
Isoform 3 (identifier: A8MT69-3) [UniParc]FASTAAdd to basket

Also known as: Variant B

The sequence of this isoform differs from the canonical sequence as follows:
     30-47: Missing.
     73-77: Missing.

Show »
Length:58
Mass (Da):6,466
Checksum:i5166DA91818795E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651V → A in AAH09571 (PubMed:15489334).Curated
Sequence conflicti73 – 731V → L in AAB53638 (Ref. 1) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei30 – 4718Missing in isoform 2 and isoform 3. 2 PublicationsVSP_033948Add
BLAST
Alternative sequencei73 – 775Missing in isoform 3. 2 PublicationsVSP_033949

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95006 mRNA. Translation: AAB53638.1.
U95007 mRNA. Translation: AAB53639.1.
AC137723 Genomic DNA. No translation available.
BC009571 mRNA. Translation: AAH09571.1.
BC011610 mRNA. Translation: AAH11610.1.
CCDSiCCDS32772.1. [A8MT69-2]
CCDS59302.1. [A8MT69-3]
CCDS59303.1. [A8MT69-1]
RefSeqiNP_001257935.1. NM_001271006.1. [A8MT69-1]
NP_001257936.1. NM_001271007.1. [A8MT69-3]
NP_659435.2. NM_144998.3. [A8MT69-2]
UniGeneiHs.37616.

Genome annotation databases

EnsembliENST00000306704; ENSP00000302951; ENSG00000169689. [A8MT69-2]
ENST00000392359; ENSP00000376168; ENSG00000169689.
ENST00000580435; ENSP00000462015; ENSG00000169689. [A8MT69-3]
GeneIDi201254.
KEGGihsa:201254.
UCSCiuc002kdc.4. human. [A8MT69-2]
uc002kdd.4. human. [A8MT69-3]
uc031rey.1. human. [A8MT69-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95006 mRNA. Translation: AAB53638.1.
U95007 mRNA. Translation: AAB53639.1.
AC137723 Genomic DNA. No translation available.
BC009571 mRNA. Translation: AAH09571.1.
BC011610 mRNA. Translation: AAH11610.1.
CCDSiCCDS32772.1. [A8MT69-2]
CCDS59302.1. [A8MT69-3]
CCDS59303.1. [A8MT69-1]
RefSeqiNP_001257935.1. NM_001271006.1. [A8MT69-1]
NP_001257936.1. NM_001271007.1. [A8MT69-3]
NP_659435.2. NM_144998.3. [A8MT69-2]
UniGeneiHs.37616.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DRAX-ray2.41E/F/G/H1-81[»]
4DRBX-ray2.63J/K/L/M/N/O1-81[»]
4E44X-ray2.10B/D1-81[»]
4E45X-ray2.00B/D/G/I/L/N1-81[»]
4NDYX-ray7.00B/D/H/L/M/N/U/V/W/X8-81[»]
4NE1X-ray6.50B/D/H/L/M/N/U/V/W/X/Z/b/d/h/i/j/o/p/q/r8-81[»]
4NE3X-ray1.80B8-81[»]
4NE5X-ray2.50B/D/F/H8-81[»]
4NE6X-ray2.10B/D8-81[»]
ProteinModelPortaliA8MT69.
SMRiA8MT69. Positions 8-81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128376. 18 interactions.
IntActiA8MT69. 3 interactions.

Proteomic databases

MaxQBiA8MT69.
PaxDbiA8MT69.
PRIDEiA8MT69.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000306704; ENSP00000302951; ENSG00000169689. [A8MT69-2]
ENST00000392359; ENSP00000376168; ENSG00000169689.
ENST00000580435; ENSP00000462015; ENSG00000169689. [A8MT69-3]
GeneIDi201254.
KEGGihsa:201254.
UCSCiuc002kdc.4. human. [A8MT69-2]
uc002kdd.4. human. [A8MT69-3]
uc031rey.1. human. [A8MT69-1]

Organism-specific databases

CTDi201254.
GeneCardsiGC17M079977.
H-InvDBHIX0014271.
HGNCiHGNC:11422. STRA13.
HPAiHPA027348.
MIMi615128. gene.
neXtProtiNX_A8MT69.
PharmGKBiPA36223.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG71130.
GeneTreeiENSGT00390000002725.
HOGENOMiHOG000231242.
InParanoidiA8MT69.
KOiK15360.
PhylomeDBiA8MT69.

Enzyme and pathway databases

ReactomeiREACT_22186. Deposition of new CENPA-containing nucleosomes at the centromere.

Miscellaneous databases

GenomeRNAii201254.
NextBioi90103.
PROiA8MT69.
SOURCEiSearch...

Gene expression databases

BgeeiA8MT69.
CleanExiHS_STRA13.
ExpressionAtlasiA8MT69. baseline and differential.
GenevisibleiA8MT69. HS.

Family and domain databases

InterProiIPR018552. CENP-X.
[Graphical view]
PfamiPF09415. CENP-X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of two human D9 transcripts."
    Scott L.M., Collins S.J.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
  2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-81 (ISOFORM 3).
    Tissue: Placenta and Skin.
  4. "The CENP-S complex is essential for the stable assembly of outer kinetochore structure."
    Amano M., Suzuki A., Hori T., Backer C., Okawa K., Cheeseman I.M., Fukagawa T.
    J. Cell Biol. 186:173-182(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH APITD1.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FA CORE COMPLEX, INTERACTION WITH FANCM AND APITD1, SUBCELLULAR LOCATION, DNA-BINDING, FUNCTION.
  6. "MHF1-MHF2, a histone-fold-containing protein complex, participates in the Fanconi anemia pathway via FANCM."
    Singh T.R., Saro D., Ali A.M., Zheng X.-F., Du C., Killen M.W., Sachpatzidis A., Wahengbam K., Pierce A.J., Xiong Y., Sung P., Meetei A.R.
    Mol. Cell 37:879-886(2010)
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FA CORE COMPLEX, INTERACTION WITH FANCM AND APITD1, SUBCELLULAR LOCATION, DNA-BINDING, FUNCTION.
  7. "CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."
    Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
    Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCENPX_HUMAN
AccessioniPrimary (citable) accession number: A8MT69
Secondary accession number(s): O00281
, O00282, Q96DD4, Q96F51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: July 22, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.