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A8MT69

- CENPX_HUMAN

UniProt

A8MT69 - CENPX_HUMAN

Protein

Centromere protein X

Gene

STRA13

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation.3 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. kinetochore assembly Source: InterPro
    3. mitotic nuclear division Source: UniProtKB-KW
    4. positive regulation of protein ubiquitination Source: UniProtKB
    5. replication fork processing Source: UniProtKB
    6. resolution of meiotic recombination intermediates Source: UniProtKB

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA repair, Mitosis

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Centromere protein X
    Short name:
    CENP-X
    Alternative name(s):
    FANCM-interacting histone fold protein 2
    Fanconi anemia-associated polypeptide of 10 kDa
    Retinoic acid-inducible gene D9 protein homolog
    Stimulated by retinoic acid gene 13 protein homolog
    Gene namesi
    Name:STRA13
    Synonyms:CENPX, FAAP10, MHF2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:11422. STRA13.

    Subcellular locationi

    Nucleus. Chromosomecentromere. Chromosomecentromerekinetochore
    Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis.

    GO - Cellular componenti

    1. condensed chromosome kinetochore Source: UniProtKB-SubCell
    2. FANCM-MHF complex Source: UniProtKB
    3. Fanconi anaemia nuclear complex Source: UniProtKB

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Fanconi anemia

    Organism-specific databases

    PharmGKBiPA36223.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8181Centromere protein XPRO_0000337180Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiA8MT69.
    PaxDbiA8MT69.
    PRIDEiA8MT69.

    Expressioni

    Gene expression databases

    ArrayExpressiA8MT69.
    BgeeiA8MT69.
    CleanExiHS_STRA13.
    GenevestigatoriA8MT69.

    Organism-specific databases

    HPAiHPA027348.

    Interactioni

    Subunit structurei

    Belongs to the multisubunit FA complex composed of APITD1/CENPS, FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, FANCM, FAAP24 and STRA13/CENPX. Interacts with APITD1/CENPS, FANCM and FAAP24. Component of a discrete APITD1/CENPS complex composed of at least APITD1/CENPS and STRA13/CENPX; this complex binds DNA. The heterodimer composed of APITD1/CENPS and STRA13/CENPX can dimerize to form a heterotetramer. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APITD1Q8N2Z96EBI-5529694,EBI-5529649

    Protein-protein interaction databases

    BioGridi128376. 7 interactions.
    IntActiA8MT69. 1 interaction.
    STRINGi9606.ENSP00000302951.

    Structurei

    Secondary structure

    1
    81
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi12 – 209
    Helixi32 – 5928
    Beta strandi63 – 653
    Helixi67 – 8014

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4DRAX-ray2.41E/F/G/H1-81[»]
    4DRBX-ray2.63J/K/L/M/N/O1-81[»]
    4E44X-ray2.10B/D1-81[»]
    4E45X-ray2.00B/D/G/I/L/N1-81[»]
    4NDYX-ray7.00B/D/H/L/M/N/U/V/W/X8-81[»]
    4NE1X-ray6.50B/D/H/L/M/N/U/V/W/X/Z/b/d/h/i/j/o/p/q/r8-81[»]
    4NE3X-ray1.80B8-81[»]
    4NE5X-ray2.50B/D/F/H8-81[»]
    4NE6X-ray2.10B/D8-81[»]
    ProteinModelPortaliA8MT69.
    SMRiA8MT69. Positions 8-81.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CENPX family.Curated

    Phylogenomic databases

    eggNOGiNOG71130.
    HOGENOMiHOG000231242.
    InParanoidiA8MT69.
    KOiK15360.
    PhylomeDBiA8MT69.

    Family and domain databases

    InterProiIPR018552. CENP-X.
    [Graphical view]
    PfamiPF09415. CENP-X. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A8MT69-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEGAGAGSGF RKELVSRLLH LHFKDDKTKV SGDALQLMVE LLKVFVVEAA   50
    VRGVRQAQAE DALRVDVDQL EKVLPQLLLD F 81
    Length:81
    Mass (Da):8,959
    Last modified:December 4, 2007 - v1
    Checksum:i0BA47A4F0FFD2978
    GO
    Isoform 2 (identifier: A8MT69-2) [UniParc]FASTAAdd to Basket

    Also known as: Variant A

    The sequence of this isoform differs from the canonical sequence as follows:
         30-47: Missing.

    Show »
    Length:63
    Mass (Da):7,017
    Checksum:iB751CBF3D27E6B1A
    GO
    Isoform 3 (identifier: A8MT69-3) [UniParc]FASTAAdd to Basket

    Also known as: Variant B

    The sequence of this isoform differs from the canonical sequence as follows:
         30-47: Missing.
         73-77: Missing.

    Show »
    Length:58
    Mass (Da):6,466
    Checksum:i5166DA91818795E5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651V → A in AAH09571. (PubMed:15489334)Curated
    Sequence conflicti73 – 731V → L in AAB53638. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei30 – 4718Missing in isoform 2 and isoform 3. 2 PublicationsVSP_033948Add
    BLAST
    Alternative sequencei73 – 775Missing in isoform 3. 2 PublicationsVSP_033949

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U95006 mRNA. Translation: AAB53638.1.
    U95007 mRNA. Translation: AAB53639.1.
    AC137723 Genomic DNA. No translation available.
    BC009571 mRNA. Translation: AAH09571.1.
    BC011610 mRNA. Translation: AAH11610.1.
    CCDSiCCDS32772.1. [A8MT69-2]
    CCDS59302.1. [A8MT69-3]
    CCDS59303.1. [A8MT69-1]
    RefSeqiNP_001257935.1. NM_001271006.1. [A8MT69-1]
    NP_001257936.1. NM_001271007.1. [A8MT69-3]
    NP_659435.2. NM_144998.3. [A8MT69-2]
    UniGeneiHs.37616.

    Genome annotation databases

    EnsembliENST00000306704; ENSP00000302951; ENSG00000169689. [A8MT69-2]
    ENST00000392359; ENSP00000376168; ENSG00000169689. [A8MT69-1]
    ENST00000580435; ENSP00000462015; ENSG00000169689. [A8MT69-3]
    GeneIDi201254.
    KEGGihsa:201254.
    UCSCiuc002kdc.4. human. [A8MT69-2]
    uc002kdd.4. human. [A8MT69-3]
    uc031rey.1. human. [A8MT69-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U95006 mRNA. Translation: AAB53638.1 .
    U95007 mRNA. Translation: AAB53639.1 .
    AC137723 Genomic DNA. No translation available.
    BC009571 mRNA. Translation: AAH09571.1 .
    BC011610 mRNA. Translation: AAH11610.1 .
    CCDSi CCDS32772.1. [A8MT69-2 ]
    CCDS59302.1. [A8MT69-3 ]
    CCDS59303.1. [A8MT69-1 ]
    RefSeqi NP_001257935.1. NM_001271006.1. [A8MT69-1 ]
    NP_001257936.1. NM_001271007.1. [A8MT69-3 ]
    NP_659435.2. NM_144998.3. [A8MT69-2 ]
    UniGenei Hs.37616.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4DRA X-ray 2.41 E/F/G/H 1-81 [» ]
    4DRB X-ray 2.63 J/K/L/M/N/O 1-81 [» ]
    4E44 X-ray 2.10 B/D 1-81 [» ]
    4E45 X-ray 2.00 B/D/G/I/L/N 1-81 [» ]
    4NDY X-ray 7.00 B/D/H/L/M/N/U/V/W/X 8-81 [» ]
    4NE1 X-ray 6.50 B/D/H/L/M/N/U/V/W/X/Z/b/d/h/i/j/o/p/q/r 8-81 [» ]
    4NE3 X-ray 1.80 B 8-81 [» ]
    4NE5 X-ray 2.50 B/D/F/H 8-81 [» ]
    4NE6 X-ray 2.10 B/D 8-81 [» ]
    ProteinModelPortali A8MT69.
    SMRi A8MT69. Positions 8-81.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 128376. 7 interactions.
    IntActi A8MT69. 1 interaction.
    STRINGi 9606.ENSP00000302951.

    Proteomic databases

    MaxQBi A8MT69.
    PaxDbi A8MT69.
    PRIDEi A8MT69.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000306704 ; ENSP00000302951 ; ENSG00000169689 . [A8MT69-2 ]
    ENST00000392359 ; ENSP00000376168 ; ENSG00000169689 . [A8MT69-1 ]
    ENST00000580435 ; ENSP00000462015 ; ENSG00000169689 . [A8MT69-3 ]
    GeneIDi 201254.
    KEGGi hsa:201254.
    UCSCi uc002kdc.4. human. [A8MT69-2 ]
    uc002kdd.4. human. [A8MT69-3 ]
    uc031rey.1. human. [A8MT69-1 ]

    Organism-specific databases

    CTDi 201254.
    GeneCardsi GC17M079977.
    H-InvDB HIX0014271.
    HGNCi HGNC:11422. STRA13.
    HPAi HPA027348.
    neXtProti NX_A8MT69.
    PharmGKBi PA36223.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG71130.
    HOGENOMi HOG000231242.
    InParanoidi A8MT69.
    KOi K15360.
    PhylomeDBi A8MT69.

    Miscellaneous databases

    GenomeRNAii 201254.
    NextBioi 90103.
    PROi A8MT69.

    Gene expression databases

    ArrayExpressi A8MT69.
    Bgeei A8MT69.
    CleanExi HS_STRA13.
    Genevestigatori A8MT69.

    Family and domain databases

    InterProi IPR018552. CENP-X.
    [Graphical view ]
    Pfami PF09415. CENP-X. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of two human D9 transcripts."
      Scott L.M., Collins S.J.
      Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
    2. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-81 (ISOFORM 3).
      Tissue: Placenta and Skin.
    4. "The CENP-S complex is essential for the stable assembly of outer kinetochore structure."
      Amano M., Suzuki A., Hori T., Backer C., Okawa K., Cheeseman I.M., Fukagawa T.
      J. Cell Biol. 186:173-182(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH APITD1.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FA CORE COMPLEX, INTERACTION WITH FANCM AND APITD1, SUBCELLULAR LOCATION, DNA-BINDING, FUNCTION.
    6. "MHF1-MHF2, a histone-fold-containing protein complex, participates in the Fanconi anemia pathway via FANCM."
      Singh T.R., Saro D., Ali A.M., Zheng X.-F., Du C., Killen M.W., Sachpatzidis A., Wahengbam K., Pierce A.J., Xiong Y., Sung P., Meetei A.R.
      Mol. Cell 37:879-886(2010)
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FA CORE COMPLEX, INTERACTION WITH FANCM AND APITD1, SUBCELLULAR LOCATION, DNA-BINDING, FUNCTION.
    7. "CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."
      Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
      Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCENPX_HUMAN
    AccessioniPrimary (citable) accession number: A8MT69
    Secondary accession number(s): O00281
    , O00282, Q96DD4, Q96F51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3