A8MT69 (CENPX_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centromere protein X
Short name=CENP-X

Alternative name(s):
FANCM-interacting histone fold protein 2
Fanconi anemia-associated polypeptide of 10 kDa
Retinoic acid-inducible gene D9 protein homolog
Stimulated by retinoic acid gene 13 protein homolog

Gene names

Name:	STRA13
Synonyms:	CENPX, FAAP10, MHF2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	81 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation. Ref.4 Ref.5 Ref.6
Subunit structure	Belongs to the multisubunit FA complex composed of APITD1/CENPS, FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, FANCM, FAAP24 and STRA13/CENPX. Interacts with APITD1/CENPS, FANCM and FAAP24. Component of a discrete APITD1/CENPS complex composed of at least APITD1/CENPS and STRA13/CENPX; this complex binds DNA. The heterodimer composed of APITD1/CENPS and STRA13/CENPX can dimerize to form a heterotetramer. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Ref.4 Ref.5 Ref.6 Ref.7
Subcellular location	Nucleus. Chromosome › centromere. Chromosome › centromere › kinetochore. Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Ref.5 Ref.6
Sequence similarities	Belongs to the CENPX family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA repair Mitosis
Cellular component	Centromere Chromosome Kinetochore Nucleus
Coding sequence diversity	Alternative splicing
Disease	Fanconi anemia
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of protein ubiquitination Traceable author statement PubMed 20347428. Source: UniProtKB replication fork processing Inferred from mutant phenotype PubMed 20347428. Source: UniProtKB resolution of meiotic recombination intermediates Inferred from mutant phenotype PubMed 20347428. Source: UniProtKB
Cellular_component	FANCM-MHF complex Inferred from direct assay PubMed 20347428. Source: UniProtKB Fanconi anaemia nuclear complex Inferred from direct assay PubMed 20347428PubMed 20347429. Source: UniProtKB condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from direct assay PubMed 20347429. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APITD1	Q8N2Z9	2	EBI-5529694,EBI-5529649

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A8MT69-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A8MT69-2)

Also known as: Variant A;

The sequence of this isoform differs from the canonical sequence as follows:
     30-47: Missing.

Isoform 3 (identifier: A8MT69-3)

Also known as: Variant B;

The sequence of this isoform differs from the canonical sequence as follows:
     30-47: Missing.
     73-77: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 81

81

Centromere protein X

PRO_0000337180

Natural variations

Alternative sequence

30 – 47

18

Missing in isoform 2 and isoform 3.

VSP_033948

Alternative sequence

73 – 77

5

Missing in isoform 3.

VSP_033949

Experimental info

Sequence conflict

65

1

V → A in AAH09571. Ref.3

Sequence conflict

73

1

V → L in AAB53638. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

81

Helix Strand Turn

Details...

Helix

12 – 20

9

Helix

32 – 59

28

Beta strand

63 – 65

3

Helix

67 – 80

14

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 0BA47A4F0FFD2978	FASTA	81	8,959
	10 20 30 40 50 60 MEGAGAGSGF RKELVSRLLH LHFKDDKTKV SGDALQLMVE LLKVFVVEAA VRGVRQAQAE 70 80 DALRVDVDQL EKVLPQLLLD F
	Isoform 2 (Variant A) [UniParc]. Checksum: B751CBF3D27E6B1A	FASTA	63	7,017
	10 20 30 40 50 60 MEGAGAGSGF RKELVSRLLH LHFKDDKTKE AAVRGVRQAQ AEDALRVDVD QLEKVLPQLL LDF
	Isoform 3 (Variant B) [UniParc]. Checksum: 5166DA91818795E5	FASTA	58	6,466
	10 20 30 40 50 MEGAGAGSGF RKELVSRLLH LHFKDDKTKE AAVRGVRQAQ AEDALRVDVD QLEKLLDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of two human D9 transcripts." Scott L.M., Collins S.J. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3).
[2]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-81 (ISOFORM 3). Tissue: Placenta and Skin.
[4]	"The CENP-S complex is essential for the stable assembly of outer kinetochore structure." Amano M., Suzuki A., Hori T., Backer C., Okawa K., Cheeseman I.M., Fukagawa T. J. Cell Biol. 186:173-182(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH APITD1.
[5]	"A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability." Yan Z., Delannoy M., Ling C., Daee D., Osman F., Muniandy P.A., Shen X., Oostra A.B., Du H., Steltenpool J., Lin T., Schuster B., Decaillet C., Stasiak A., Stasiak A.Z., Stone S., Hoatlin M.E., Schindler D. , Woodcock C.L., Joenje H., Sen R., de Winter J.P., Li L., Seidman M.M., Whitby M.C., Myung K., Constantinousend A., Wang W. Mol. Cell 37:865-878(2010) Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FA CORE COMPLEX, INTERACTION WITH FANCM AND APITD1, SUBCELLULAR LOCATION, DNA-BINDING, FUNCTION.
[6]	"MHF1-MHF2, a histone-fold-containing protein complex, participates in the Fanconi anemia pathway via FANCM." Singh T.R., Saro D., Ali A.M., Zheng X.-F., Du C., Killen M.W., Sachpatzidis A., Wahengbam K., Pierce A.J., Xiong Y., Sung P., Meetei A.R. Mol. Cell 37:879-886(2010) Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FA CORE COMPLEX, INTERACTION WITH FANCM AND APITD1, SUBCELLULAR LOCATION, DNA-BINDING, FUNCTION.
[7]	"CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold." Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T. Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U95006 mRNA. Translation: AAB53638.1.
U95007 mRNA. Translation: AAB53639.1.
AC137723 Genomic DNA. No translation available.
BC009571 mRNA. Translation: AAH09571.1.
BC011610 mRNA. Translation: AAH11610.1.

IPI

IPI00010887.
IPI00795077.
IPI00795646.

RefSeq

NP_001257935.1. NM_001271006.1.
NP_001257936.1. NM_001271007.1.
NP_659435.2. NM_144998.3.

UniGene

Hs.37616.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4DRA	X-ray	2.41	E/F/G/H	1-81	[»]
4DRB	X-ray	2.63	J/K/L/M/N/O	1-81	[»]

ProteinModelPortal

A8MT69.

ModBase

Search...

Protein-protein interaction databases

IntAct

A8MT69. 1 interaction.

STRING

9606.ENSP00000302951.

Proteomic databases

PaxDb

A8MT69.

PRIDE

A8MT69.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000306704; ENSP00000302951; ENSG00000169689.
ENST00000392359; ENSP00000376168; ENSG00000169689.
ENST00000580435; ENSP00000462015; ENSG00000169689.

GeneID

201254.

KEGG

hsa:201254.

UCSC

uc002kdc.3. human.

Organism-specific databases

CTD

201254.

GeneCards

GC17M079977.

H-InvDB

HIX0014271.

HGNC

HGNC:11422. STRA13.

HPA

HPA027348.

neXtProt

NX_A8MT69.

PharmGKB

PA36223.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG71130.

HOGENOM

HOG000231242.

InParanoid

A8MT69.

KO

K15360.

OMA

KVFVVEA.

Gene expression databases

Bgee

A8MT69.

CleanEx

HS_STRA13.

Genevestigator

A8MT69.

Family and domain databases

InterPro

IPR018552. CENP-S_centromere_X.
[Graphical view]

Pfam

PF09415. CENP-X. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

201254.

NextBio

90103.

Entry information

Entry name

CENPX_HUMAN

Accession

Primary (citable) accession number: A8MT69
Secondary accession number(s): O00281 , O00282, Q96DD4, Q96F51

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.