ID   A8MRP9_ARATH            Unreviewed;       328 AA.
AC   A8MRP9;
DT   04-DEC-2007, integrated into UniProtKB/TrEMBL.
DT   04-DEC-2007, sequence version 1.
DT   13-SEP-2023, entry version 98.
DE   RecName: Full=Methylthioribose-1-phosphate isomerase {ECO:0000256|HAMAP-Rule:MF_03119};
DE            Short=M1Pi {ECO:0000256|HAMAP-Rule:MF_03119};
DE            Short=MTR-1-P isomerase {ECO:0000256|HAMAP-Rule:MF_03119};
DE            EC=5.3.1.23 {ECO:0000256|HAMAP-Rule:MF_03119};
DE   AltName: Full=S-methyl-5-thioribose-1-phosphate isomerase {ECO:0000256|HAMAP-Rule:MF_03119};
DE   AltName: Full=Translation initiation factor eIF-2B subunit alpha/beta/delta-like protein {ECO:0000256|HAMAP-Rule:MF_03119};
GN   Name=MTI1 {ECO:0000313|EMBL:AEC05978.1, ECO:0000313|TAIR:AT2G05830};
GN   Synonyms=5-METHYLTHIORIBOSE KINASE 1 {ECO:0000313|EMBL:AEC05978.1};
GN   OrderedLocusNames=At2g05830 {ECO:0000313|Araport:AT2G05830,
GN   ECO:0000313|EMBL:AEC05978.1};
GN   ORFNames=T6P5.3 {ECO:0000313|EMBL:AEC05978.1}, T6P5_3
GN   {ECO:0000313|EMBL:AEC05978.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000313|EMBL:AEC05978.1, ECO:0000313|Proteomes:UP000006548};
RN   [1] {ECO:0000313|EMBL:AEC05978.1, ECO:0000313|Proteomes:UP000006548}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S., Shea T.P., Benito M.I., Town C.D.,
RA   Fujii C.Y., Mason T., Bowman C.L., Barnstead M., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2] {ECO:0000313|Proteomes:UP000006548}
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: Catalyzes the interconversion of methylthioribose-1-phosphate
CC       (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).
CC       {ECO:0000256|HAMAP-Rule:MF_03119}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-
CC         D-ribulose 1-phosphate; Xref=Rhea:RHEA:19989, ChEBI:CHEBI:58533,
CC         ChEBI:CHEBI:58548; EC=5.3.1.23; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_03119};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage
CC       pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate:
CC       step 1/6. {ECO:0000256|HAMAP-Rule:MF_03119}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03119}.
CC       Nucleus {ECO:0000256|HAMAP-Rule:MF_03119}.
CC   -!- SIMILARITY: Belongs to the eIF-2B alpha/beta/delta subunits family.
CC       MtnA subfamily. {ECO:0000256|HAMAP-Rule:MF_03119}.
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DR   EMBL; CP002685; AEC05978.1; -; Genomic_DNA.
DR   RefSeq; NP_001077883.1; NM_001084414.1.
DR   AlphaFoldDB; A8MRP9; -.
DR   SMR; A8MRP9; -.
DR   IntAct; A8MRP9; 7.
DR   iPTMnet; A8MRP9; -.
DR   DNASU; 815134; -.
DR   EnsemblPlants; AT2G05830.4; AT2G05830.4; AT2G05830.
DR   GeneID; 815134; -.
DR   Gramene; AT2G05830.4; AT2G05830.4; AT2G05830.
DR   Araport; AT2G05830; -.
DR   TAIR; AT2G05830; MTI1.
DR   PhylomeDB; A8MRP9; -.
DR   UniPathway; UPA00904; UER00874.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; A8MRP9; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0046523; F:S-methyl-5-thioribose-1-phosphate isomerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019509; P:L-methionine salvage from methylthioadenosine; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019284; P:L-methionine salvage from S-adenosylmethionine; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.420; translation initiation factor eif-2b, domain 1; 1.
DR   HAMAP; MF_01678; Salvage_MtnA; 1.
DR   InterPro; IPR000649; IF-2B-related.
DR   InterPro; IPR005251; IF-M1Pi.
DR   InterPro; IPR042529; IF_2B-like_C.
DR   InterPro; IPR011559; Initiation_fac_2B_a/b/d.
DR   InterPro; IPR027363; M1Pi_N.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   NCBIfam; TIGR00524; eIF-2B_rel; 1.
DR   NCBIfam; TIGR00512; salvage_mtnA; 1.
DR   PANTHER; PTHR43475; METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE; 1.
DR   PANTHER; PTHR43475:SF1; METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE; 1.
DR   Pfam; PF01008; IF-2B; 1.
DR   SUPFAM; SSF100950; NagB/RpiA/CoA transferase-like; 1.
PE   1: Evidence at protein level;
KW   Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605, ECO:0000256|HAMAP-
KW   Rule:MF_03119}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03119};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_03119};
KW   Methionine biosynthesis {ECO:0000256|ARBA:ARBA00023167, ECO:0000256|HAMAP-
KW   Rule:MF_03119}; Nucleus {ECO:0000256|HAMAP-Rule:MF_03119};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:A8MRP9,
KW   ECO:0007829|ProteomicsDB:A8MRP9};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006548};
KW   Transferase {ECO:0000313|EMBL:AEC05978.1}.
FT   ACT_SITE        207
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_03119"
FT   SITE            127
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_03119"
SQ   SEQUENCE   328 AA;  34535 MW;  96E1C36B596462FF CRC64;
     MEMVVRGAPA IAIAAALSLA VEVFNFHGFD GSASDAVAFL ENKLDYLVSS RPTAVNLADA
     ALKLKHVIAK ALATATEAKS IFKAYIEASE DMLEDDVVSN KAIGNFGLSL LRQQAKNPDK
     LSVLTHCNTG SLATAGYGTA LGVIRALHTQ GILERAYCTE TRPFNQGSRL TAFELVHEKI
     PATLIADSAA AALMKDGRVD GVIVGADRVA SNGDTANKIG TYSLALCAKH HGIPFYVAAP
     LTSVDLSLSS GKEIVIEERS PKELMHTHGG LGERIAAPGI SVWNPAFDMT PAELIAGIIT
     EKGVITKNGN DTFDISSFAK KITGNSSR
//