Reviewed, UniProtKB/Swiss-Prot A8MRC7 (B3GT2_ARATH)

Names and origin

Protein names

Recommended name:
    Probable beta-1,3-galactosyltransferase 2
    EC=2.4.1.-

Gene names

Name:	B3GALT2
Ordered Locus Names:	At1g05170
ORF Names:	YUP8H12.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue By similarity.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A8MRC7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Derived from EST data.

Isoform 2 (identifier: A8MRC7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     70-72: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Probable beta-1,3-galactosyltransferase 2		PRO_0000359412
Regions
	Transmembrane	13 – 35	23	Signal-anchor for type II membrane protein Potential
Natural variations
	Alternative sequence	70 – 72	3	Missing in isoform 2.		VSP_036143

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: F58ECFF90B2D60E5	FASTA	407	46,162
	10 20 30 40 50 60 MSAKIKGEYS SRSFVSRKWT ILLCLGSFCV GMFFTNRMWN IPESKGMSHP SVTEAERLKL 70 80 90 100 110 120 VSEGCNPKAL YQKEVKRDPQ ALFGEVANTH IALQTLDKTI SSLEMELAAA RSVQESLQNG 130 140 150 160 170 180 APLSDDMGKK QPQEQRRFLM VVGINTAFSS RKRRDSIRAT WMPQGEKRKR LEEEKGIIIR 190 200 210 220 230 240 FVIGHSATTG GILDRAIEAE DRKHGDFLRL DHVEGYLELS GKTKTYFSTA FSMWDADFYV 250 260 270 280 290 300 KVDDDVHVNI ATLGETLVRH RKKPRVYIGC MKSGPVLSQK GVRYHEPEYW KFGENGNKYF 310 320 330 340 350 360 RHATGQLYAI SRDLASYISI NQHVLHKYAN EDVSLGAWFI GIDVKHIDDR RLCCGTPPDC 370 380 390 400 EWKAQAGNIC VASFDWSCSG ICRSADRIKE VHRRCGEGEK ALWSATF
	Isoform 2. Checksum: F7AC0DF45BA362C0	FASTA	404	45,757
	10 20 30 40 50 60 MSAKIKGEYS SRSFVSRKWT ILLCLGSFCV GMFFTNRMWN IPESKGMSHP SVTEAERLKL 70 80 90 100 110 120 VSEGCNPKAK EVKRDPQALF GEVANTHIAL QTLDKTISSL EMELAAARSV QESLQNGAPL 130 140 150 160 170 180 SDDMGKKQPQ EQRRFLMVVG INTAFSSRKR RDSIRATWMP QGEKRKRLEE EKGIIIRFVI 190 200 210 220 230 240 GHSATTGGIL DRAIEAEDRK HGDFLRLDHV EGYLELSGKT KTYFSTAFSM WDADFYVKVD 250 260 270 280 290 300 DDVHVNIATL GETLVRHRKK PRVYIGCMKS GPVLSQKGVR YHEPEYWKFG ENGNKYFRHA 310 320 330 340 350 360 TGQLYAISRD LASYISINQH VLHKYANEDV SLGAWFIGID VKHIDDRRLC CGTPPDCEWK 370 380 390 400 AQAGNICVAS FDWSCSGICR SADRIKEVHR RCGEGEKALW SATF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[3]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC000098 Genomic DNA. Translation: AAB71461.1. AF389287 mRNA. Translation: AAK63859.1. AY113007 mRNA. Translation: AAM47315.1.
IPI	IPI00537667. IPI00846604.
PIR	A86186.
RefSeq	NP_001077462.1.
UniGene	At.10413
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	839292.
GenomeReviews	Gene locus AT1G05170 in contig CT485782_GR.
KEGG	ath:AT1G05170.
Organism-specific databases
TAIR	At1g05170.
Phylogenomic databases
OMA	A8MRC7. ICKSADR.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GT2_ARATH

Accession

Primary (citable) accession number: A8MRC7
Secondary accession number(s): O23050

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	December 4, 2007
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)