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A8MR93 (ALG12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
EC=2.4.1.260
Alternative name(s):
Alpha-1,6-mannosyltransferase ALG12
Asparagine-linked glycosylation protein 12
EMS-mutagenized BRI1 suppressor 4
Gene names
Name:ALG12
Synonyms:EBS4
Ordered Locus Names:At1g02145
ORF Names:T6A9
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for N-linked oligosaccharide assembly. Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor dolichol-PP-Man7GlcNAc2. Ref.5

Catalytic activity

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate. Ref.5

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.

Miscellaneous

In the absence of ALG12 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrat tolerant.

Sequence similarities

Belongs to the glycosyltransferase 22 family.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A8MR93-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A8MR93-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     260-380: Missing.
Isoform 3 (identifier: A8MR93-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-322: Missing.
Isoform 4 (identifier: A8MR93-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     71-74: Missing.
Isoform 5 (identifier: A8MR93-5)

The sequence of this isoform differs from the canonical sequence as follows:
     71-74: Missing.
Isoform 6 (identifier: A8MR93-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 497497Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
PRO_0000412586

Regions

Transmembrane10 – 3021Helical; Potential
Transmembrane71 – 9121Helical; Potential
Transmembrane92 – 11221Helical; Potential
Transmembrane125 – 14521Helical; Potential
Transmembrane154 – 17421Helical; Potential
Transmembrane178 – 19821Helical; Potential
Transmembrane215 – 23521Helical; Potential
Transmembrane263 – 28523Helical; Potential
Transmembrane292 – 31221Helical; Potential
Transmembrane316 – 33621Helical; Potential
Transmembrane346 – 36621Helical; Potential

Amino acid modifications

Glycosylation2531N-linked (GlcNAc...) Potential
Glycosylation4351N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 322322Missing in isoform 3.
VSP_041715
Alternative sequence1 – 4444Missing in isoform 2, isoform 4 and isoform 6.
VSP_041716
Alternative sequence71 – 744Missing in isoform 4 and isoform 5.
VSP_041717
Alternative sequence260 – 380121Missing in isoform 2.
VSP_041718

Experimental info

Mutagenesis381E → K in ebs4-3; incomplete assembly of glycans and compromised endoplasmic reticulum-associated degradation (ERAD) of defective proteins. Ref.5
Mutagenesis3071S → P in ebs4-2; incomplete assembly of glycans and compromised endoplasmic reticulum-associated degradation (ERAD) of defective proteins. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 7A37B1757AA8BF88

FASTA49757,192
        10         20         30         40         50         60 
MPTDSKMAKF LQSYGYDLIL GSVAAIYVVM APYTKVEESF NVQSMHDILY HRHHLDSYDH 

        70         80         90        100        110        120 
LEFPGVVPRT FIGAFIVSVF ASPVVSIISC LGFPKVYSLV AARLVLGCII LSTLRFFRIQ 

       130        140        150        160        170        180 
IKKKFGNQVE TFFVLFTSLQ FHFLFYCTRP LPNILALGLV NLAYGNWLKG NFYPALSFLI 

       190        200        210        220        230        240 
FATVIFRCDT MLLLGPIGLE LLLTKSISFW KALKYCVGTA LLAVGLTIFV DSIMWKKFVW 

       250        260        270        280        290        300 
PEFEVFWFNS ILNRSSDWGT HSIHWYFTSA LPRSLLVAYP LSLLGTLVDR RVPFFIVPVL 

       310        320        330        340        350        360 
SFVILYSKLP HKELRFIISS VPMFNLSAAV AASRIYNNRK KTIWKLVNMV MLAFFAISAG 

       370        380        390        400        410        420 
CTVVTFMASY YNYPSGYALK RLHQIGHPAN VAGEEWVHID TFGAMNGISR FCEDDFPWRY 

       430        440        450        460        470        480 
SKEEEIVVEE LRNRNFTYLV NEHSSVDGYK CLFYEEGFER LELRRGFPPI VLVKKAKVYL 

       490 
HREMKKEDPF HKKWPGC

« Hide

Isoform 2 [UniParc].

Checksum: CABCB5138FF9B750
Show »

FASTA33238,590
Isoform 3 [UniParc].

Checksum: AB0C1CFEBABA0372
Show »

FASTA17520,464
Isoform 4 [UniParc].

Checksum: 039866FA595997F3
Show »

FASTA44951,928
Isoform 5 [UniParc].

Checksum: AEDA7E983D33032F
Show »

FASTA49356,804
Isoform 6 [UniParc].

Checksum: 26A3941197EAA7EA
Show »

FASTA45352,317

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
Plant Biotechnol. J. 4:317-324(2006) [PubMed: 17147637] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: cv. Columbia.
[5]"Mutations of an alpha1,6 mannosyltransferase inhibit endoplasmic reticulum-associated degradation of defective brassinosteroid receptors in Arabidopsis."
Hong Z., Jin H., Fitchette A.C., Xia Y., Monk A.M., Faye L., Li J.
Plant Cell 21:3792-3802(2009) [PubMed: 20023196] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF GLU-38 AND SER-307.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC064879 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE27389.1.
CP002684 Genomic DNA. Translation: AEE27390.1.
CP002684 Genomic DNA. Translation: AEE27391.1.
CP002684 Genomic DNA. Translation: AEE27392.1.
DQ492198 mRNA. Translation: ABF59127.1.
DQ492199 mRNA. Translation: ABF59128.1.
AK227725 mRNA. Translation: BAE99711.1.
IPIIPI00775582.
IPI00775644.
IPI00846482.
IPI00892322.
IPI01027883.
IPI01027886.
RefSeqNP_001077446.1. NM_001083977.4.
NP_001077447.2. NM_001083978.2.
NP_001077448.1. NM_001083979.2.
NP_001117214.1. NM_001123742.1.
UniGeneAt.71188.

3D structure databases

ProteinModelPortalA8MR93.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8MR93.

Protein family/group databases

CAZyGT22. Glycosyltransferase Family 22.

Proteomic databases

PRIDEA8MR93.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G02145.3; AT1G02145.3; AT1G02145.
GeneID5007658.
GenomeReviewsGene locus AT1G02145 in contig CT485782_GR.
KEGGath:AT1G02145.

Organism-specific databases

TAIRAt1g02145.

Phylogenomic databases

eggNOGKOG2516.
GeneTreeEPGT00050000009885.
HOGENOMHBG385438.
PhylomeDBA8MR93.
ProtClustDBCLSN2694980.

Gene expression databases

GenevestigatorA8MR93.

Family and domain databases

InterProIPR005599. GPI_mannosylTrfase.
[Graphical view]
KOK03847.
PANTHERPTHR22760. Alg9_trans. 1 hit.
PfamPF03901. Glyco_transf_22. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALG12_ARATH
AccessionPrimary (citable) accession number: A8MR93
Secondary accession number(s): F4HVW9 expand/collapse secondary AC list , F4HVX0, Q0WT37, Q1G329, Q1G330
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: December 4, 2007
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents