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Protein

Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase

Gene

ALG12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for N-linked oligosaccharide assembly. Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor dolichol-PP-Man7GlcNAc2.1 Publication

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: TAIR
  • protein N-linked glycosylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:GQT-2223-MONOMER.
ARA:GQT-2225-MONOMER.
ARA:GQT-2226-MONOMER.
BRENDAi2.4.1.260. 399.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT22. Glycosyltransferase Family 22.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase (EC:2.4.1.260)
Alternative name(s):
Alpha-1,6-mannosyltransferase ALG12
Asparagine-linked glycosylation protein 12
EMS-mutagenized BRI1 suppressor 4
Gene namesi
Name:ALG12
Synonyms:EBS4
Ordered Locus Names:At1g02145
ORF Names:T6A9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G02145.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Transmembranei71 – 9121HelicalSequence analysisAdd
BLAST
Transmembranei92 – 11221HelicalSequence analysisAdd
BLAST
Transmembranei125 – 14521HelicalSequence analysisAdd
BLAST
Transmembranei154 – 17421HelicalSequence analysisAdd
BLAST
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Transmembranei215 – 23521HelicalSequence analysisAdd
BLAST
Transmembranei263 – 28523HelicalSequence analysisAdd
BLAST
Transmembranei292 – 31221HelicalSequence analysisAdd
BLAST
Transmembranei316 – 33621HelicalSequence analysisAdd
BLAST
Transmembranei346 – 36621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381E → K in ebs4-3; incomplete assembly of glycans and compromised endoplasmic reticulum-associated degradation (ERAD) of defective proteins. 1 Publication
Mutagenesisi307 – 3071S → P in ebs4-2; incomplete assembly of glycans and compromised endoplasmic reticulum-associated degradation (ERAD) of defective proteins. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferasePRO_0000412586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence analysis
Glycosylationi435 – 4351N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiA8MR93.
PRIDEiA8MR93.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G02145.3.

Structurei

3D structure databases

ProteinModelPortaliA8MR93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 22 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2516. Eukaryota.
ENOG410XR38. LUCA.
HOGENOMiHOG000265945.
InParanoidiA8MR93.
KOiK03847.
OMAiHADVLTC.
OrthoDBiEOG093607RF.
PhylomeDBiA8MR93.

Family and domain databases

InterProiIPR005599. GPI_mannosylTrfase.
[Graphical view]
PANTHERiPTHR22760. PTHR22760. 1 hit.
PfamiPF03901. Glyco_transf_22. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A8MR93-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTDSKMAKF LQSYGYDLIL GSVAAIYVVM APYTKVEESF NVQSMHDILY
60 70 80 90 100
HRHHLDSYDH LEFPGVVPRT FIGAFIVSVF ASPVVSIISC LGFPKVYSLV
110 120 130 140 150
AARLVLGCII LSTLRFFRIQ IKKKFGNQVE TFFVLFTSLQ FHFLFYCTRP
160 170 180 190 200
LPNILALGLV NLAYGNWLKG NFYPALSFLI FATVIFRCDT MLLLGPIGLE
210 220 230 240 250
LLLTKSISFW KALKYCVGTA LLAVGLTIFV DSIMWKKFVW PEFEVFWFNS
260 270 280 290 300
ILNRSSDWGT HSIHWYFTSA LPRSLLVAYP LSLLGTLVDR RVPFFIVPVL
310 320 330 340 350
SFVILYSKLP HKELRFIISS VPMFNLSAAV AASRIYNNRK KTIWKLVNMV
360 370 380 390 400
MLAFFAISAG CTVVTFMASY YNYPSGYALK RLHQIGHPAN VAGEEWVHID
410 420 430 440 450
TFGAMNGISR FCEDDFPWRY SKEEEIVVEE LRNRNFTYLV NEHSSVDGYK
460 470 480 490
CLFYEEGFER LELRRGFPPI VLVKKAKVYL HREMKKEDPF HKKWPGC
Length:497
Mass (Da):57,192
Last modified:December 4, 2007 - v1
Checksum:i7A37B1757AA8BF88
GO
Isoform 2 (identifier: A8MR93-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     260-380: Missing.

Show »
Length:332
Mass (Da):38,590
Checksum:iCABCB5138FF9B750
GO
Isoform 3 (identifier: A8MR93-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-322: Missing.

Show »
Length:175
Mass (Da):20,464
Checksum:iAB0C1CFEBABA0372
GO
Isoform 4 (identifier: A8MR93-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     71-74: Missing.

Show »
Length:449
Mass (Da):51,928
Checksum:i039866FA595997F3
GO
Isoform 5 (identifier: A8MR93-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     71-74: Missing.

Show »
Length:493
Mass (Da):56,804
Checksum:iAEDA7E983D33032F
GO
Isoform 6 (identifier: A8MR93-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Show »
Length:453
Mass (Da):52,317
Checksum:i26A3941197EAA7EA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 322322Missing in isoform 3. 1 PublicationVSP_041715Add
BLAST
Alternative sequencei1 – 4444Missing in isoform 2, isoform 4 and isoform 6. 1 PublicationVSP_041716Add
BLAST
Alternative sequencei71 – 744Missing in isoform 4 and isoform 5. 1 PublicationVSP_041717
Alternative sequencei260 – 380121Missing in isoform 2. 1 PublicationVSP_041718Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC064879 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE27389.1.
CP002684 Genomic DNA. Translation: AEE27390.1.
CP002684 Genomic DNA. Translation: AEE27391.1.
CP002684 Genomic DNA. Translation: AEE27392.1.
DQ492198 mRNA. Translation: ABF59127.1.
DQ492199 mRNA. Translation: ABF59128.1.
AK227725 mRNA. Translation: BAE99711.1.
RefSeqiNP_001077446.1. NM_001083977.4. [A8MR93-5]
NP_001077447.2. NM_001083978.2. [A8MR93-2]
NP_001077448.1. NM_001083979.2. [A8MR93-1]
NP_001117214.1. NM_001123742.1. [A8MR93-6]
UniGeneiAt.71188.

Genome annotation databases

EnsemblPlantsiAT1G02145.3; AT1G02145.3; AT1G02145. [A8MR93-1]
GeneIDi5007658.
KEGGiath:AT1G02145.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC064879 Genomic DNA. No translation available.
CP002684 Genomic DNA. Translation: AEE27389.1.
CP002684 Genomic DNA. Translation: AEE27390.1.
CP002684 Genomic DNA. Translation: AEE27391.1.
CP002684 Genomic DNA. Translation: AEE27392.1.
DQ492198 mRNA. Translation: ABF59127.1.
DQ492199 mRNA. Translation: ABF59128.1.
AK227725 mRNA. Translation: BAE99711.1.
RefSeqiNP_001077446.1. NM_001083977.4. [A8MR93-5]
NP_001077447.2. NM_001083978.2. [A8MR93-2]
NP_001077448.1. NM_001083979.2. [A8MR93-1]
NP_001117214.1. NM_001123742.1. [A8MR93-6]
UniGeneiAt.71188.

3D structure databases

ProteinModelPortaliA8MR93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G02145.3.

Protein family/group databases

CAZyiGT22. Glycosyltransferase Family 22.

Proteomic databases

PaxDbiA8MR93.
PRIDEiA8MR93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G02145.3; AT1G02145.3; AT1G02145. [A8MR93-1]
GeneIDi5007658.
KEGGiath:AT1G02145.

Organism-specific databases

TAIRiAT1G02145.

Phylogenomic databases

eggNOGiKOG2516. Eukaryota.
ENOG410XR38. LUCA.
HOGENOMiHOG000265945.
InParanoidiA8MR93.
KOiK03847.
OMAiHADVLTC.
OrthoDBiEOG093607RF.
PhylomeDBiA8MR93.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciARA:GQT-2223-MONOMER.
ARA:GQT-2225-MONOMER.
ARA:GQT-2226-MONOMER.
BRENDAi2.4.1.260. 399.
ReactomeiR-ATH-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiA8MR93.

Family and domain databases

InterProiIPR005599. GPI_mannosylTrfase.
[Graphical view]
PANTHERiPTHR22760. PTHR22760. 1 hit.
PfamiPF03901. Glyco_transf_22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG12_ARATH
AccessioniPrimary (citable) accession number: A8MR93
Secondary accession number(s): F4HVW9
, F4HVX0, Q0WT37, Q1G329, Q1G330
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: December 4, 2007
Last modified: September 7, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In the absence of ALG12 activity, the N-glycans transferred to proteins are aberrant, indicating that the oligosaccharyltransferase (OST) complex is substrate-tolerant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.