A8MQ27 (NEU1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase NEURL1B
EC=6.3.2.-

Alternative name(s):
Neuralized-2
Short name=NEUR2
Neuralized-like protein 1B
Neuralized-like protein 3

Gene names

Name:	NEURL1B
Synonyms:	NEURL3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase involved in regulation of the Notch pathway through influencing the stability and activity of several Notch ligands. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with JAG1, DLL1 and DLL4. Ref.1
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Highest expression in brain, prostate and small intestine. In the brain the levels are higher in fetal than in adult stage. In the adult brain the highest levels are detected in the olfactory system, cerebellar cortex, optic nerve and the frontal lobe. Ref.1
Sequence similarities	Contains 2 NHR (neuralized homology repeat) domains. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Notch signaling pathway Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Notch signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A8MQ27-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A8MQ27-2)

Also known as: delta-NHR1;

The sequence of this isoform differs from the canonical sequence as follows:
     11-192: Missing.

Isoform 3 (identifier: A8MQ27-3)

Also known as: delta-NHR2;

The sequence of this isoform differs from the canonical sequence as follows:
     191-430: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	E3 ubiquitin-protein ligase NEURL1B		PRO_0000349382
Regions
	Domain	38 – 194	157	NHR 1
	Domain	279 – 433	155	NHR 2
	Zinc finger	503 – 543	41	RING-type
	Compositional bias	252 – 258	7	Poly-Ala
	Compositional bias	437 – 491	55	Ser-rich
Natural variations
	Alternative sequence	11 – 192	182	Missing in isoform 2.		VSP_044404
	Alternative sequence	191 – 430	240	Missing in isoform 3.		VSP_044405

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 228B459336279423	FASTA	555	59,270
	10 20 30 40 50 60 MGNTVHRTLP DPSPPARLLA TRPCCGPGPE RRPVLGEAPR FHAQAKGKNV RLDGHSRRAT 70 80 90 100 110 120 RRNSFCNGVT FTQRPIRLYE QVRLRLVAVR PGWSGALRFG FTAHDPSLMS AQDIPKYACP 130 140 150 160 170 180 DLVTRPGYWA KALPENLALR DTVLAYWADR HGRVFYSVND GEPVLFHCGV AVGGPLWALI 190 200 210 220 230 240 DVYGITDEVQ LLESAFADTL TPARLSQARF SACLPPSSHD AANFDNNELE NNQVVAKLGH 250 260 270 280 290 300 LALGRAPGPP PADAAAAAIP CGPRERPRPA SSPALLEADL RFHATRGPDV SLSADRKVAC 310 320 330 340 350 360 APRPDGGRTL VFSERPLRPG ESLFVEVGRP GLAAPGALAF GITSCDPGVL RPNELPADPD 370 380 390 400 410 420 ALLDRKEYWV VARAGPVPSG GDALSFTLRP GGDVLLGING RPRGRLLCVD TTQALWAFFA 430 440 450 460 470 480 VRGGVAGQLR LLGTLQSSPA TTTPSGSLSG SQDDSDSDMT FSVNQSSSAS ESSLVTAPSS 490 500 510 520 530 540 PLSPPVSPVF SPPEPAGIKN GECTVCFDGE VDTVIYTCGH MCLCHSCGLR LKRQARACCP 550 ICRRPIKDVI KIYRP
	Isoform 2 (delta-NHR1) [UniParc]. Checksum: 2EA7D029C5E62A91	FASTA	373	39,058
	10 20 30 40 50 60 MGNTVHRTLP ESAFADTLTP ARLSQARFSA CLPPSSHDAA NFDNNELENN QVVAKLGHLA 70 80 90 100 110 120 LGRAPGPPPA DAAAAAIPCG PRERPRPASS PALLEADLRF HATRGPDVSL SADRKVACAP 130 140 150 160 170 180 RPDGGRTLVF SERPLRPGES LFVEVGRPGL AAPGALAFGI TSCDPGVLRP NELPADPDAL 190 200 210 220 230 240 LDRKEYWVVA RAGPVPSGGD ALSFTLRPGG DVLLGINGRP RGRLLCVDTT QALWAFFAVR 250 260 270 280 290 300 GGVAGQLRLL GTLQSSPATT TPSGSLSGSQ DDSDSDMTFS VNQSSSASES SLVTAPSSPL 310 320 330 340 350 360 SPPVSPVFSP PEPAGIKNGE CTVCFDGEVD TVIYTCGHMC LCHSCGLRLK RQARACCPIC 370 RRPIKDVIKI YRP
	Isoform 3 (delta-NHR2) [UniParc]. Checksum: C7AC47D5CBD9B54B	FASTA	315	34,230
	10 20 30 40 50 60 MGNTVHRTLP DPSPPARLLA TRPCCGPGPE RRPVLGEAPR FHAQAKGKNV RLDGHSRRAT 70 80 90 100 110 120 RRNSFCNGVT FTQRPIRLYE QVRLRLVAVR PGWSGALRFG FTAHDPSLMS AQDIPKYACP 130 140 150 160 170 180 DLVTRPGYWA KALPENLALR DTVLAYWADR HGRVFYSVND GEPVLFHCGV AVGGPLWALI 190 200 210 220 230 240 DVYGITDEVQ LLGTLQSSPA TTTPSGSLSG SQDDSDSDMT FSVNQSSSAS ESSLVTAPSS 250 260 270 280 290 300 PLSPPVSPVF SPPEPAGIKN GECTVCFDGE VDTVIYTCGH MCLCHSCGLR LKRQARACCP 310 ICRRPIKDVI KIYRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Neuralized-2: Expression in human and rodents and interaction with Delta-like ligands." Rullinkov G., Tamme R., Sarapuu A., Lauren J., Sepp M., Palm K., Timmusk T. Biochem. Biophys. Res. Commun. 389:420-425(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, INTERACTION WITH DLL1 AND DLL4, TISSUE SPECIFICITY.
[2]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	GQ414757 mRNA. Translation: ACV53563.1. GQ414758 mRNA. Translation: ACV53564.1. GQ414759 mRNA. Translation: ACV53565.1. AC027309 Genomic DNA. No translation available.
IPI	IPI00871148. IPI00952681. IPI00985052.
RefSeq	NP_001136123.1. NM_001142651.1.
UniGene	Hs.91521.
3D structure databases
ProteinModelPortal	A8MQ27.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000358815.
PTM databases
PhosphoSite	A8MQ27.
Proteomic databases
PaxDb	A8MQ27.
PRIDE	A8MQ27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000369800; ENSP00000358815; ENSG00000214357. ENST00000520919; ENSP00000429797; ENSG00000214357. ENST00000522853; ENSP00000430001; ENSG00000214357.
GeneID	54492.
KEGG	hsa:54492.
UCSC	uc003mbt.3. human.
Organism-specific databases
CTD	54492.
GeneCards	GC05P172068.
HGNC	HGNC:35422. NEURL1B.
HPA	HPA037612.
neXtProt	NX_A8MQ27.
PharmGKB	PA164723819.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG145384.
HOGENOM	HOG000233742.
InParanoid	A8MQ27.
KO	K01931.
OMA	AIPCGPR.
OrthoDB	EOG4PK284.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	A8MQ27.
Bgee	A8MQ27.
Genevestigator	A8MQ27.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR006573. Neu_Z. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF07177. Neuralized. 2 hits. [Graphical view]
SMART	SM00588. NEUZ. 2 hits. SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS51065. NHR. 2 hits. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NEURL1B. human.
GenomeRNAi	54492.
NextBio	35484036.

Entry information

Entry name

NEU1B_HUMAN

Accession

Primary (citable) accession number: A8MQ27
Secondary accession number(s): C9DQJ5, C9DQJ6, C9DQJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.