A8MLI7 (PUR5_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	Clos_0539

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_1000083448

Sequences

	Sequence		Length	Mass (Da)
	A8MLI7 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 431AE717D3CDB5C9	FASTA	341	36,549
	10 20 30 40 50 60 MKITYKSAGV DVDEGQRAVQ LMKNHVKSTF NSNVLADIGG FGGLFSLNIK DYKEPVLVAG 70 80 90 100 110 120 TDGVGTKLKI AFMMDKHDTI GQDCVAMCVN DILCQGGKPL FFLDYVATGK LVGEKIAQIV 130 140 150 160 170 180 GGIAEGCRKA GSALIGGETA EMPGLYAENE YDLAGFAVGI VDREQIITGA NIKAGDIILG 190 200 210 220 230 240 LPSNGIHSNG YSLVRKLFFE VLDMKITDYV EEFGTTLGEE LLKPTKIYVK EILSLIEAIP 250 260 270 280 290 300 VKGISHITGG GFFENIPRIL PENVDAKINV DAWQIPPIFK FMAEKGNMSK DDIFGTFNMG 310 320 330 340 IGMVVVVSKE DVNGAIAVLN KAGQEAHIIG EVVEGAKQVI L

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW18101.1.
RefSeq	YP_001512097.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MLI7.
SMR	A8MLI7. Positions 16-337.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MLI7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5678036.
GenomeReviews	Gene locus Clos_0539 in contig CP000853_GR.
KEGG	aoe:Clos_0539.
PATRIC	20866179. VBIAlkOre124042_0558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG531222.
OMA	GIDMIAM.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_0539-MONOMER.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_ALKOO

Accession

Primary (citable) accession number: A8MLI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program