A8MLB7 (SYC_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Clos_0469

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000332785
Regions
	Motif	29 – 39	11	"HIGH" region HAMAP MF_00041
	Motif	264 – 268	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	27	1	Zinc By similarity
	Metal binding	207	1	Zinc By similarity
	Metal binding	232	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Binding site	267	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MLB7 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 94456F50DC365CCF	FASTA	464	53,471
	10 20 30 40 50 60 MKLYNTLTRK KEEFIPLEEG KVRMYACGPT VYNYFHIGNA RTFMVFDALR RYLIYRGYDV 70 80 90 100 110 120 TFVQNFTDVD DKIIRRANEE GVAPEEISER FIKEYFYDAG TLGIEKADIH PKVTENMAEI 130 140 150 160 170 180 IAFVAKLVEK GHAYESNGDV YFDVSKYEEY GKLSKQSLED LQAGARIEIN EYKKNPLDFA 190 200 210 220 230 240 LWKSAKAGEP SWESPWGNGR PGWHIECSAM AKRYLGETID IHGGGGDLVF PHHENEIAQS 250 260 270 280 290 300 EACSGKKFAN YWLHVGYLNV DNKKMSKSLN NFFTPREISE EFDLENLRFF MLSAHYRNPI 310 320 330 340 350 360 NFSRDLLEAA KNGLDRLYTG KNNLEYLLEN AAEREVSEEE KNFIHGLQSF KNQFIDAVDD 370 380 390 400 410 420 DFNTADGIAV IFDLVREINS HISEKNSKKA VEASYDLLME LTGVLGLLKR EAEDLEEEIE 430 440 450 460 RLIAERQQAR KDKNFALSDK IRDDLKEKGI VLEDTAQGVK WRKL

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW18031.1.
RefSeq	YP_001512027.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MLB7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MLB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5677005.
GenomeReviews	Gene locus Clos_0469 in contig CP000853_GR.
KEGG	aoe:Clos_0469.
PATRIC	20866039. VBIAlkOre124042_0488.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_0469-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ALKOO

Accession

Primary (citable) accession number: A8MLB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program