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A8MLB5

- SYE_ALKOO

UniProt

A8MLB5 - SYE_ALKOO

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei260 – 2601ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAORE350688:GHBG-485-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:Clos_0467
    OrganismiAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
    Taxonomic identifieri350688 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
    ProteomesiUP000000269: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 495495Glutamate--tRNA ligasePRO_1000057190Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi350688.Clos_0467.

    Structurei

    3D structure databases

    ProteinModelPortaliA8MLB5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi9 – 1911"HIGH" regionAdd
    BLAST
    Motifi257 – 2615"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK09698.
    OMAiFFVEEVD.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A8MLB5-1 [UniParc]FASTAAdd to Basket

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    MSVRVRFAPS PTGFVHIGSL RTALYNYLFA KKSNGKYILR VEDTDQSRYV    50
    EGAIEGMLRS MAWAGVHHDE GVVFTGETLG QKGDFGPYIQ SERLEIYKKY 100
    MDILLEKGHA YYCFCSKERL DEVREKQKVE GLNAGYDGHC RNLSKEEIEE 150
    KLNAGQPYVI RLRLPENKDI QFHDIVRGDV VMNTADLDDQ VLMKSDGFPT 200
    YHFAVVVDDH LMEITHVIRG EEWLPSTPKH VYLYEAFGWE APQYVHLPNI 250
    LNADRKKLSK RQGDVAVEDF LKKGYLPEGL VNYIALVGWS PEDNNEIFSM 300
    EELEANFSLE RVSKSGGVFD VHKLNWVNGH YIRESSVERI TDLAIPHLIE 350
    AGYITESDAQ ERYEWLKDLV SVLQDRISYV AEIKEKAGIF FNAEIELKDE 400
    ESKELIQVEH LPQLLDVLKA KVEAAEVIDE EFGKSVFKEI QKETGIKGPN 450
    LFKPIRVVLT GEVHGPDLPL VMKVLGSKNI LDRIEYVYDL IQSMK 495
    Length:495
    Mass (Da):56,679
    Last modified:December 4, 2007 - v1
    Checksum:i6C94413C6AC28043
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000853 Genomic DNA. Translation: ABW18029.1.
    RefSeqiYP_001512025.1. NC_009922.1.

    Genome annotation databases

    EnsemblBacteriaiABW18029; ABW18029; Clos_0467.
    GeneIDi5677003.
    KEGGiaoe:Clos_0467.
    PATRICi20866035. VBIAlkOre124042_0486.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000853 Genomic DNA. Translation: ABW18029.1 .
    RefSeqi YP_001512025.1. NC_009922.1.

    3D structure databases

    ProteinModelPortali A8MLB5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 350688.Clos_0467.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABW18029 ; ABW18029 ; Clos_0467 .
    GeneIDi 5677003.
    KEGGi aoe:Clos_0467.
    PATRICi 20866035. VBIAlkOre124042_0486.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K09698.
    OMAi FFVEEVD.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci AORE350688:GHBG-485-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: OhILAs.

    Entry informationi

    Entry nameiSYE_ALKOO
    AccessioniPrimary (citable) accession number: A8MLB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3