A8MLB3 (SYP_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Clos_0465

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Proline--tRNA ligase HAMAP MF_01571		PRO_1000069189

Sequences

	Sequence		Length	Mass (Da)
	A8MLB3 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: AF6CC931623FFACE	FASTA	480	55,575
	10 20 30 40 50 60 MSKKEKQFVE EITPMEVDFA QWYTDVIKKT DLVDYSPVKG FMVIKPYGYA IWENIQNYMD 70 80 90 100 110 120 KRFKETGHKN CYFPLLIPES LLKKEAEHVE GFAPEVAWVT HGGNEELAER LCVRPTSETI 130 140 150 160 170 180 ICEMYSKWLT SYRDLPFLYN QWCSVVRWEK STRPFLRTSE FLWQEGHTLH ETYEEAQAET 190 200 210 220 230 240 LQMLNIYRET AENLLAMPVV IGQKSEKEKF AGAYATYTME ALMHDGKALQ AGTSHNLGQH 250 260 270 280 290 300 FTTAFDITYS DRNGELKHPY HTSWGVSTRL IGGIIMVHGD NRGLVLPPGI APTQVVIVPV 310 320 330 340 350 360 ASHKEGVLDK ANELRDRLKD KFRVELDDRD NYSPGWKFNE WEMKGVPIRI EIGPRDIENN 370 380 390 400 410 420 QAMLFRRDEL EKDAVSLDDL EEAVEKLLED INNNLLYKAK MMRDEKTYIV KTFDEMKEVM 430 440 450 460 470 480 EIKPGFVKAM WCGERACEEH VKAETGVTIR CIPFEQENLG HTCAFCGKEA KHMVYLAKAY

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW18027.1.
RefSeq	YP_001512023.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MLB3.
SMR	A8MLB3. Positions 10-480.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MLB3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5677892.
GenomeReviews	Gene locus Clos_0465 in contig CP000853_GR.
KEGG	aoe:Clos_0465.
PATRIC	20866031. VBIAlkOre124042_0484.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG334108.
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_0465-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ALKOO

Accession

Primary (citable) accession number: A8MLB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program