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Reviewed, UniProtKB/Swiss-Prot A8ML31 (ISPE_ALKOO)

Last modified November 3, 2009. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name: ispE
Ordered Locus Names: Clos_0285
OrganismAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]
Taxonomic identifier350688 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2922924-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
PRO_1000092059

Regions

Nucleotide binding95 – 10511ATP Potential

Sites

Active site111 By similarity
Active site1371 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8ML31-1 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 203423BF926B838F

FASTA29232,187
        10         20         30         40         50         60 
MKQIYLKSRA KINLSLDVRR KREDGYHEVE MIMQQIDLYD NILIRERMDS EIVLSTNCIF 

        70         80         90        100        110        120 
IPTTSSNIAY KAAHKLKQRL DITRGIDIFI DKQIPVSAGL AGGSSNAAAV LMGLNHLWSL 

       130        140        150        160        170        180 
GLSTKELMEI GVTIGADVPF CLLGGTALAE GIGEVLTPIN SDIKNTWIVL VKPAISVSTG 

       190        200        210        220        230        240 
DVYGSLDLSK IVDRPPTAQL LEAIKEGNIY DVSSKMCNVL ETVTVNKYPI ITEIKKKMME 

       250        260        270        280        290 
YNALGAMMSG SGPTVFGIFK SYERAKSAYE HLSLFYKQSY MVQTYNGGTE IG 

« Hide

References

[1]"Complete genome of Alkaliphilus oremlandii OhILAs."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

CP000853 Genomic DNA. Translation: ABW17848.1.
RefSeqYP_001511844.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID5678591.
GenomeReviewsGene locus Clos_0285 in contig CP000853_GR.
KEGGaoe:Clos_0285.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMADGYHEVE.

Family and domain databases

HAMAPMF_00061.
[Tree]
InterProIPR006204. GHMP_kinase.
IPR013750. GHMP_kinase_C.
IPR004424. IspE.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
Gene3DG3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHERPTHR20861:SF2. IspE. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
TIGRFAMsTIGR00154. ispE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_ALKOO
AccessionPrimary (citable) accession number: A8ML31
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 4, 2007
Last modified: November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents