Reviewed, UniProtKB/Swiss-Prot A8ML24 (AMPA_ALKOO)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Clos_0278

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000058384
Sites
	Active site	279	1	Potential
	Active site	353	1	Potential
	Metal binding	267	1	Manganese 2 By similarity
	Metal binding	272	1	Manganese 1 By similarity
	Metal binding	272	1	Manganese 2 By similarity
	Metal binding	290	1	Manganese 2 By similarity
	Metal binding	349	1	Manganese 1 By similarity
	Metal binding	351	1	Manganese 1 By similarity
	Metal binding	351	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ML24-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 835EF5EB2C9707F7	FASTA	499	53,414
	10 20 30 40 50 60 MKIQVMKDQI KEIMADAIVI GIYEGTKSLN DNLKSMDLQL DGIITEMISS EAFKGKEGET 70 80 90 100 110 120 LVIYSLGRIP AKKIMLLGLG KETDLKEDTI RRLTAKVVRE AEAMKAKVVA MTAIGLDRGI 130 140 150 160 170 180 APELVGQCMM EGADLAAYKF DKYKTTDRNS GEGVQELYLL NEEESINKDL EKGFQTGAKL 190 200 210 220 230 240 AQGTIIARNL VNEPSNVLTP TAMADKAIEI ANNHGLEISI LEKEDMEKLG MGSFLGVTKG 250 260 270 280 290 300 SEEPPKLIAI KYFGNKEDEE IIGLVGKGLT FDSGGISLKP GAGMDAMKSD MGGAAAVLGA 310 320 330 340 350 360 MDVIGALKPK VNVIAIVGAC ENMPSGKAYK PGDILTSMNG KTIEILNTDA EGRLVLIDCI 370 380 390 400 410 420 TYALKQGATR IVDLATLTGA CIVALGHVTT ALVSNDDDFV QQMYLAAERA GEKVWQLPSF 430 440 450 460 470 480 PEYKELIKSD IADLKNVGDK GAGTITAGLF LGEFVEGRPW IHMDIAGTAM ALGTKGYYAK 490 GGSGVGVRTL YHLVKSMEK

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000853 Genomic DNA. Translation: ABW17841.1.
RefSeq	YP_001511837.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M17.010.
Genome annotation databases
GeneID	5678182.
GenomeReviews	Gene locus Clos_0278 in contig CP000853_GR.
KEGG	aoe:Clos_0278.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CVVGLMH.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ALKOO

Accession

Primary (citable) accession number: A8ML24

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)