A8MKX8 (A8MKX8_ALKOO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase HAMAP MF_00555
EC=6.3.1.1 HAMAP MF_00555

Alternative name(s):
Asparagine synthetase A HAMAP MF_00555

Gene names

Name:	asnA HAMAP MF_00555
Ordered Locus Names:	Clos_0232

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP] EMBL ABW17795.1

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily. HAMAP MF_00555

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis HAMAP MF_00555
Cellular component	Cytoplasm HAMAP MF_00555
Ligand	ATP-binding HAMAP MF_00555 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00555 EMBL ABW17795.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A8MKX8 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: BEFA8AA0B7861EB4	FASTA	337	39,023
	10 20 30 40 50 60 MKERLIVPEG YEPSLSIKET EVAIKQLKDF FERELAKTLN LTRVSAPLFV CPETGMNDNL 70 80 90 100 110 120 NGVERPVSFD IKDIKGQYVE IVHSLAKWKR MALGRYGFEV GEGLYTDMDA IRRDEDLDNI 130 140 150 160 170 180 HSIYVDQWDW EKIITKEERT EAKLKEIVER IYEVFKRTGD YVEKLYPELK NILPEKITFI 190 200 210 220 230 240 TSQELEDRYP GLSSKDREHA IAKEYGAVFI MKIGGKLLSG ERHDGRAPDY DDWELNGDIL 250 260 270 280 290 300 FWNPLLNIGL ELSSMGIRVD EDTLEKQLSI AGCEDRKKLD FHRLVLEKKL PYTVGGGIGQ 310 320 330 SRICMFFLQK AHIGEVQASI WSKEMIDFCE KHHMPLL

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW17795.1.
RefSeq	YP_001511791.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MKX8.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MKX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5676270.
GenomeReviews	Gene locus Clos_0232 in contig CP000853_GR.
KEGG	aoe:Clos_0232.
PATRIC	20865547. VBIAlkOre124042_0244.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A8MKX8_ALKOO

Accession

Primary (citable) accession number: A8MKX8

Entry history

Integrated into UniProtKB/TrEMBL:	December 4, 2007
Last sequence update:	December 4, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)