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A8MK93 (THIM_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:Clos_2694
OrganismAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]
Taxonomic identifier350688 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_1000058757

Sites

Binding site461Substrate; via amide nitrogen By similarity
Binding site1221ATP By similarity
Binding site1691ATP By similarity
Binding site1961Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A8MK93 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 5A0F14F070AD70B0

FASTA27128,694
        10         20         30         40         50         60 
MELKNELCTV LEQIKEKTPL VHHITNYVTV NDCANITLAI GGSPVMADDH KEVEDMVSIA 

        70         80         90        100        110        120 
SAVVLNIGTL NERTIESFVL AGKKANELNI PVILDPVGAG ATAFRSQTIE KILKEVKLSV 

       130        140        150        160        170        180 
LRGNMSEIKN IYGTGTQTKG VDSVDSSLDG GKEIAISLAK KLSCTVVITG EVDIVSDGNK 

       190        200        210        220        230        240 
TYAIQNGHKA LSSITGTGCM SASLIGACCG TGKGILQGAI LGTMIMGIAG EKANERLKVH 

       250        260        270 
EGLGSFKVYL MDAVSNFDQD DIRKRGKVDE I 

« Hide

References

[1]"Complete genome of Alkaliphilus oremlandii OhILAs."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OhILAs.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000853 Genomic DNA. Translation: ABW20225.1.
RefSeqYP_001514221.1. NC_009922.1.

3D structure databases

ProteinModelPortalA8MK93.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING350688.Clos_2694.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABW20225; ABW20225; Clos_2694.
GeneID5678747.
KEGGaoe:Clos_2694.
PATRIC20870874. VBIAlkOre124042_2814.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMALPLMATH.
OrthoDBEOG628F8M.
ProtClustDBPRK09355.

Enzyme and pathway databases

BioCycAORE350688:GHBG-2807-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_ALKOO
AccessionPrimary (citable) accession number: A8MK93
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 4, 2007
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways