Reviewed, UniProtKB/Swiss-Prot A8MK48 (MURC_ALKOO)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Clos_2649

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000336811
Regions
	Nucleotide binding	119 – 125	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8MK48-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 4B65363FF72302B5	FASTA	465	52,339
	10 20 30 40 50 60 MTTIDMNQDL GKHIHLIGIG GISMSAIAEI LLNQGYRVSG SDMNDSNIIN KLRAHGAEIF 70 80 90 100 110 120 IGHNEDNVKD PDLVVYTAAI KEHNPERMAA KERNIPQIDR AEMLGIIMKN YKKAIAVSGS 130 140 150 160 170 180 HGKTTTTSLI SLIMEYSNLD PTIMVGGELD EIGGNIKIGN SEHFITEACE YVESFLKFYP 190 200 210 220 230 240 FIGIILNIDE DHLDYFKDLN HIKEAFRKFI NLIPQEGFLI ANGDDANVQE ILHSIECNLI 250 260 270 280 290 300 TYGLDSSNTF VARNISFTND GYPIFTVDFK GESMGTFELN IPGKHNIYNA LASIATAYLL 310 320 330 340 350 360 GVEPSEIQNH IKKFRGIHRR FDILGEVKGC KIVDDYAHHP VEISATLEAA KNYPHNKIWC 370 380 390 400 410 420 VFQPHTFSRT KALLEDFSRS FTNADHIIIT DIYAAREQDT GEVHSEILAN MIQKNNMHSQ 430 440 450 460 VRYMKDFEDI AAYIYDHMEP GDLVLTMGAG NVYQIGQKIL QRHNE

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000853 Genomic DNA. Translation: ABW20180.1.
RefSeq	YP_001514176.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5676611.
GenomeReviews	Gene locus Clos_2649 in contig CP000853_GR.
KEGG	aoe:Clos_2649.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ALKOO

Accession

Primary (citable) accession number: A8MK48

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)