A8MK43 (PTH_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidyl-tRNA hydrolase
Short name=PTH
EC=3.1.1.29

Gene names

Name:	pth
Ordered Locus Names:	Clos_2644

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity. HAMAP MF_00083
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA. HAMAP MF_00083
Subunit structure	Monomer By similarity. HAMAP MF_00083
Subcellular location	Cytoplasm By similarity HAMAP MF_00083.
Sequence similarities	Belongs to the PTH family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Peptidyl-tRNA hydrolase HAMAP MF_00083		PRO_1000057545

Sequences

	Sequence		Length	Mass (Da)
	A8MK43 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: EC9385246CC98612	FASTA	185	20,539
	10 20 30 40 50 60 MHIIVGLGNP GKKYDATRHN IGFEAIDMLA KRNNIEVKKL KHKALCGEGT IGGNKVLLVK 70 80 90 100 110 120 PQTFMNLSGQ SLLDIVQFYK VDPKNIVVLY DDIDIPVGTL RIREKGSSGT HNGMKSIIYL 130 140 150 160 170 180 LQTDQFPRIR IGVGKPQFGD LADYVLGRFP KEEIPTMLET LERASQAVET LVKDGIAVSM NRYNG

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW20175.1.
RefSeq	YP_001514171.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MK43.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MK43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5677436.
GenomeReviews	Gene locus Clos_2644 in contig CP000853_GR.
KEGG	aoe:Clos_2644.
PATRIC	20870770. VBIAlkOre124042_2762.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG610927.
OMA	IKFKTGG.
ProtClustDB	PRK05426.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_2644-MONOMER.
Family and domain databases
HAMAP	MF_00083. Pept_tRNA_hydro_bact. [Tree]
InterPro	IPR001328. Pept_tRNA_hydro. IPR018171. Pept_tRNA_hydro_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.1470. Pept_tRNA_hydro. 1 hit.
KO	K01056.
PANTHER	PTHR17224. Pept_tRNA_hydro. 1 hit.
Pfam	PF01195. Pept_tRNA_hydro. 1 hit. [Graphical view]
SUPFAM	SSF53178. Pept_tRNA_hydro. 1 hit.
TIGRFAMs	TIGR00447. Pth. 1 hit.
PROSITE	PS01195. PEPT_TRNA_HYDROL_1. 1 hit. PS01196. PEPT_TRNA_HYDROL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTH_ALKOO

Accession

Primary (citable) accession number: A8MK43

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program