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A8MJY6 (SYL_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Clos_2587
OrganismAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]
Taxonomic identifier350688 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus

Protein attributes

Sequence length802 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 802802Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000057340

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif580 – 5845"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5831ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8MJY6 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 96F021E311FB5EC5

FASTA80292,499
        10         20         30         40         50         60 
MKQYNPKEIE SKWQNIWEER GVFHASNDKD KEKFYALIEF PYPSGQGLHV GHPRPYTALD 

        70         80         90        100        110        120 
VVSRKRRLEG YNVLYPMGWD AFGLPTENYA IKNKIHPKVV TEQNVARFKK QLQGLGMSFD 

       130        140        150        160        170        180 
WSREINTTDP EYYKWTQWIF LKLFEKGLAY KKEMSINWCT SCKVGLANEE VVNGGCERCG 

       190        200        210        220        230        240 
AEVVRKQKNQ WMLKITEYAE RLINDLDLVD YIERVKIQQK NWIGKSEGME VDFEITGGKK 

       250        260        270        280        290        300 
ITVYTTRPDT LFGSTYMVIS PEHPYIEELA AHIQNMDDLV HYREEAAKKS EFERTELVKD 

       310        320        330        340        350        360 
KTGVKIEGIE ATNPATGKQI PIFISDYVMM SYGTGAIMAV PGHDTRDWEF AKKFNLPIVE 

       370        380        390        400        410        420 
VVSGGNVDEA AYTDTEEGII VNSDFINGMQ VKEAKEKISS WLEEKGLGKR KVNYKLRDWV 

       430        440        450        460        470        480 
FSRQRYWGEP IPLVHCDCCG WVPVPESQLP VLLPEVESYE PTDNGESPLA NLRDWVETTC 

       490        500        510        520        530        540 
PKCGGKAERE TDTMPQWAGS SWYFLRYTDP HNNEELASKE NLDYWMPIDW YNGGMEHTTL 

       550        560        570        580        590        600 
HLLYSRFWHK FLYDCGVVPS CEPYQKRTSH GMILGENNEK MSKSRGNVIN PDDIVTEFGA 

       610        620        630        640        650        660 
DTLRLYEMFI GDFEKSVPWS QNGVKGCRRF LDRIWKLQEI LVDSQEMTKE LEGNIHKTIK 

       670        680        690        700        710        720 
KVTEDYETLK FNTAVASMMA LINDFYDHGT VTKGDMKTLL TLLNPVAPHI TEELWEVLGF 

       730        740        750        760        770        780 
EGHIYGTTWP IWDEAKTIDS VVEIPIQING KVKGQMVISV DFTADQVKEQ FRNDERLMAL 

       790        800 
VEGKSIVKEI YVPGKIYNIV VK 

« Hide

References

[1]"Complete genome of Alkaliphilus oremlandii OhILAs."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OhILAs.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000853 Genomic DNA. Translation: ABW20118.1.
RefSeqYP_001514114.1. NC_009922.1.

3D structure databases

ProteinModelPortalA8MJY6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING350688.Clos_2587.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABW20118; ABW20118; Clos_2587.
GeneID5676663.
KEGGaoe:Clos_2587.
PATRIC20870654. VBIAlkOre124042_2704.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMANENGVEG.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycAORE350688:GHBG-2700-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ALKOO
AccessionPrimary (citable) accession number: A8MJY6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 4, 2007
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries