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A8MJ91 (SYN_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Asparagine--tRNA ligase
EC=6.1.1.22
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS
Gene names
Name:asnS
Ordered Locus Names:Clos_2341
OrganismAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]
Taxonomic identifier350688 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534

Subunit structure

Homodimer By similarity. HAMAP MF_00534

Subcellular location

Cytoplasm HAMAP MF_00534.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processasparaginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

asparagine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 463463Asparagine--tRNA ligase HAMAP MF_00534
PRO_1000061017

Sequences

Sequence LengthMass (Da)Tools
A8MJ91 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 69FDB59885240AB3

FASTA46352,838
        10         20         30         40         50         60 
MKSILVKDIY RNTEKYAGQE IVIEGWIRTL RASKAFGFIE VNDGSFFKNI QVVFEEGLEN 

        70         80         90        100        110        120 
FAEVSKLSIS TAIIVKGKLE ITPDAKQPFE IKATSIEVEG ESDSDYPLQK KRHTFEFLRE 

       130        140        150        160        170        180 
IAHLRPRSNT FSAVFRVRSL AAFAIHQFFQ ERGFVYTHTP IITGSDAEGA GEMFRITTLD 

       190        200        210        220        230        240 
PKNPGLNEAG EVDFTKDFFG KETSLTVSGQ LQGEAYALAF RNIYTFGPTF RAENSHTARH 

       250        260        270        280        290        300 
ASEFWMIEPE IAFADLQDNM ELAEEMLKYI INYVMENAPE EMEFFNSFVD KGLLDRLNNV 

       310        320        330        340        350        360 
ATSDFGRVTY TEAIEILQKS GETFEYPVAW GTDLQTEHER YLTEKVFKKP VFVTDYPKDI 

       370        380        390        400        410        420 
KAFYMKLNED GKTVAAMDLL VPGIGEIIGG SQREDNLALL EERMDAMGLD KEEYWWYLEL 

       430        440        450        460 
RKYGSARHAG YGLGFERAIM YLTGMSNIRD VISFPRTPKS AEF

« Hide

References

[1]"Complete genome of Alkaliphilus oremlandii OhILAs."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OhILAs.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000853 Genomic DNA. Translation: ABW19873.1.
RefSeqYP_001513869.1. NC_009922.1.

3D structure databases

ProteinModelPortalA8MJ91.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8MJ91.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5677281.
GenomeReviewsGene locus Clos_2341 in contig CP000853_GR.
KEGGaoe:Clos_2341.
PATRIC20870054. VBIAlkOre124042_2453.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG745843.
OMAAIHRFFH.
ProtClustDBPRK03932.

Enzyme and pathway databases

BioCycAORE350688:CLOS_2341-MONOMER.

Family and domain databases

HAMAPMF_00534. Asn_tRNA_synth.
[Tree]
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-synth_IIb.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01893.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PTHR22594:SF6. PTHR22594:SF6. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
[Graphical view]
PRINTSPR01042. TRNASYNTHASP.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00457. AsnS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYN_ALKOO
AccessionPrimary (citable) accession number: A8MJ91
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 4, 2007
Last modified: January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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