Reviewed, UniProtKB/Swiss-Prot A8MJ87 (AROD_ALKOO)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate dehydratase
      Short name=3-dehydroquinase
    EC=4.2.1.10
Alternative name(s):
    Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	Clos_2337

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000325516
Sites
	Active site	144	1	Proton acceptor By similarity
	Active site	171	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MJ87-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: C822F8C3259FCD5A	FASTA	255	28,828
	10 20 30 40 50 60 MKKVVKVKDI TIGEGMPKIC IPILGETLEE IKEEAELLST FDFDIVEWRA DFFQFVEDMD 70 80 90 100 110 120 CIKLAMEIIE ENLGDKPMIF TLRSLKEGGK REVCEALYFE INRRVLETKN LDILDIELFH 130 140 150 160 170 180 DKDEIESLIN LAHKNGAKVI ISNHDFQQTP SKDEILSRMN RAAQLGADIV KIAVMANDTE 190 200 210 220 230 240 DVITVLDATR ILKEDYLKIP LIAIAMGKKG MITRFAGEIF GSDITFATAK KTSAPGQLSV 250 EDVKNIVQFI HKHTI

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000853 Genomic DNA. Translation: ABW19869.1.
RefSeq	YP_001513865.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5677277.
GenomeReviews	Gene locus Clos_2337 in contig CP000853_GR.
KEGG	aoe:Clos_2337.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8MJ87. MSNHDFD.
Family and domain databases
HAMAP	MF_00214. [Tree]
InterPro	IPR018508. 3-dehydroquinate_DH_AS. IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
ProDom	PD005337. DHquinase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01093. aroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_ALKOO

Accession

Primary (citable) accession number: A8MJ87

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	December 4, 2007
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)