A8MIU1 (PYRB_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	Clos_2188

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000321068

Sequences

	Sequence		Length	Mass (Da)
	A8MIU1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 88782631EA0F37AF	FASTA	331	37,306
	10 20 30 40 50 60 MPLKGKHLID PDQLTFNEIH EIINLGLDME QNPAKYSKVC EGKILGTMFF EPSTRTRLSF 70 80 90 100 110 120 ESAMLRMGGT VLGFSDVTTS SVTKGESVAD TIRVLDDYAD ILVMRHPVAG TPREASQYST 130 140 150 160 170 180 VPVINGGDGG HQHPTQTLTD LITIQKYHGE IRGLKVAFCG DLLFGRTVHS LLKTLSRFPD 190 200 210 220 230 240 MEFILISPPE LPIPEDLKTE VIEKYNVKIT ETDSLEKHMA EIDILYMTRI QKERFLNQED 250 260 270 280 290 300 YEKLKDSYIL TNSLLENAKQ DLIILHPLPR VNEIHTEVDR DPRAKYFEQA KMGVYVRMAL 310 320 330 MALLLGNIEI AAPTLTLKVK NGTIMTKFIA S

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW19723.1.
RefSeq	YP_001513719.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MIU1.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MIU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5675971.
GenomeReviews	Gene locus Clos_2188 in contig CP000853_GR.
KEGG	aoe:Clos_2188.
PATRIC	20869738. VBIAlkOre124042_2295.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	FKQSFYG.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_2188-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_ALKOO

Accession

Primary (citable) accession number: A8MIU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program