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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAORE350688:GHBG-2188-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:Clos_2127
OrganismiAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Taxonomic identifieri350688 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
ProteomesiUP000000269: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Formate--tetrahydrofolate ligasePRO_1000068788Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi350688.Clos_2127.

Structurei

3D structure databases

ProteinModelPortaliA8MIN1.
SMRiA8MIN1. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8MIN1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTDVQIAQE AKMLPIADIA AGLGIQDDEL ELYGKYKAKV SLDVFDRLKD
60 70 80 90 100
KPDGKLILVT AINPTPAGEG KTTTNVGLSM GLNKIGKKTI TALREPSLGP
110 120 130 140 150
NFGVKGGAAG GGYAQVVPME DINLHFTGDI HAITTAHNLL AALLDNHLHQ
160 170 180 190 200
GNKLNIDSRR IVWRRVLDMN DRALRNTVIG LGSRGDGVPR QDGFDITVAS
210 220 230 240 250
EIMAILCLSN SLEDLKDRIS RMVVAYNLDN QPITVNDLEA TGALSLLLKD
260 270 280 290 300
AIKPNLVQTL ENTPAFIHGG PFANIAHGCN SVLATKLGLK LADYVVTEAG
310 320 330 340 350
FGADLGAEKF FDIKCRFAGL KPDCAVIVAT VRALKNHGGV PKAELNNENL
360 370 380 390 400
EALEKGYRNL EKHIENVQKF GVPAVVAINK FPTDTEAELN FLRKHCAEMG
410 420 430 440 450
AEVVLSDVWA NGGDGGIEMA KKVVEVVESK ESNFKPLYDV NASIVEKINT
460 470 480 490 500
IAKEVYGADG VDFTKSAQTQ IKKYEDLGLD KMPICMAKTQ YSLSDDPSLI
510 520 530 540 550
GRPSGFRITV KEIRLSAGAG FLVALTGDIM VMPGLPKVPA ANHMDILESG

EIIGLF
Length:556
Mass (Da):59,818
Last modified:December 4, 2007 - v1
Checksum:iC63A71BDB4F8CAA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000853 Genomic DNA. Translation: ABW19663.1.
RefSeqiYP_001513659.1. NC_009922.1.

Genome annotation databases

EnsemblBacteriaiABW19663; ABW19663; Clos_2127.
GeneIDi5676638.
KEGGiaoe:Clos_2127.
PATRICi20869616. VBIAlkOre124042_2234.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000853 Genomic DNA. Translation: ABW19663.1.
RefSeqiYP_001513659.1. NC_009922.1.

3D structure databases

ProteinModelPortaliA8MIN1.
SMRiA8MIN1. Positions 4-554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350688.Clos_2127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW19663; ABW19663; Clos_2127.
GeneIDi5676638.
KEGGiaoe:Clos_2127.
PATRICi20869616. VBIAlkOre124042_2234.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciAORE350688:GHBG-2188-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OhILAs.

Entry informationi

Entry nameiFTHS_ALKOO
AccessioniPrimary (citable) accession number: A8MIN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 4, 2007
Last modified: January 7, 2015
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.