Reviewed, UniProtKB/Swiss-Prot A8MIN1 (FTHS_ALKOO)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase
      Short name=FHS
      Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Clos_2127

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_1000068788
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MIN1-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: C63A71BDB4F8CAA3	FASTA	556	59,818
	10 20 30 40 50 60 MKTDVQIAQE AKMLPIADIA AGLGIQDDEL ELYGKYKAKV SLDVFDRLKD KPDGKLILVT 70 80 90 100 110 120 AINPTPAGEG KTTTNVGLSM GLNKIGKKTI TALREPSLGP NFGVKGGAAG GGYAQVVPME 130 140 150 160 170 180 DINLHFTGDI HAITTAHNLL AALLDNHLHQ GNKLNIDSRR IVWRRVLDMN DRALRNTVIG 190 200 210 220 230 240 LGSRGDGVPR QDGFDITVAS EIMAILCLSN SLEDLKDRIS RMVVAYNLDN QPITVNDLEA 250 260 270 280 290 300 TGALSLLLKD AIKPNLVQTL ENTPAFIHGG PFANIAHGCN SVLATKLGLK LADYVVTEAG 310 320 330 340 350 360 FGADLGAEKF FDIKCRFAGL KPDCAVIVAT VRALKNHGGV PKAELNNENL EALEKGYRNL 370 380 390 400 410 420 EKHIENVQKF GVPAVVAINK FPTDTEAELN FLRKHCAEMG AEVVLSDVWA NGGDGGIEMA 430 440 450 460 470 480 KKVVEVVESK ESNFKPLYDV NASIVEKINT IAKEVYGADG VDFTKSAQTQ IKKYEDLGLD 490 500 510 520 530 540 KMPICMAKTQ YSLSDDPSLI GRPSGFRITV KEIRLSAGAG FLVALTGDIM VMPGLPKVPA 550 ANHMDILESG EIIGLF

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW19663.1.
RefSeq	YP_001513659.1.
3D structure databases
SMR	A8MIN1. Positions 4-554.
ModBase	Search...
Genome annotation databases
GeneID	5676638.
GenomeReviews	Gene locus Clos_2127 in contig CP000853_GR.
KEGG	aoe:Clos_2127.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG677721.
OMA	GMKGGAT.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_ALKOO

Accession

Primary (citable) accession number: A8MIN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	January 19, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)