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A8MIN1 (FTHS_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Clos_2127
OrganismAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]
Taxonomic identifier350688 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000068788

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8MIN1 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: C63A71BDB4F8CAA3

FASTA55659,818
        10         20         30         40         50         60 
MKTDVQIAQE AKMLPIADIA AGLGIQDDEL ELYGKYKAKV SLDVFDRLKD KPDGKLILVT 

        70         80         90        100        110        120 
AINPTPAGEG KTTTNVGLSM GLNKIGKKTI TALREPSLGP NFGVKGGAAG GGYAQVVPME 

       130        140        150        160        170        180 
DINLHFTGDI HAITTAHNLL AALLDNHLHQ GNKLNIDSRR IVWRRVLDMN DRALRNTVIG 

       190        200        210        220        230        240 
LGSRGDGVPR QDGFDITVAS EIMAILCLSN SLEDLKDRIS RMVVAYNLDN QPITVNDLEA 

       250        260        270        280        290        300 
TGALSLLLKD AIKPNLVQTL ENTPAFIHGG PFANIAHGCN SVLATKLGLK LADYVVTEAG 

       310        320        330        340        350        360 
FGADLGAEKF FDIKCRFAGL KPDCAVIVAT VRALKNHGGV PKAELNNENL EALEKGYRNL 

       370        380        390        400        410        420 
EKHIENVQKF GVPAVVAINK FPTDTEAELN FLRKHCAEMG AEVVLSDVWA NGGDGGIEMA 

       430        440        450        460        470        480 
KKVVEVVESK ESNFKPLYDV NASIVEKINT IAKEVYGADG VDFTKSAQTQ IKKYEDLGLD 

       490        500        510        520        530        540 
KMPICMAKTQ YSLSDDPSLI GRPSGFRITV KEIRLSAGAG FLVALTGDIM VMPGLPKVPA 

       550 
ANHMDILESG EIIGLF 

« Hide

References

[1]"Complete genome of Alkaliphilus oremlandii OhILAs."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OhILAs.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000853 Genomic DNA. Translation: ABW19663.1.
RefSeqYP_001513659.1. NC_009922.1.

3D structure databases

ProteinModelPortalA8MIN1.
SMRA8MIN1. Positions 4-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING350688.Clos_2127.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABW19663; ABW19663; Clos_2127.
GeneID5676638.
KEGGaoe:Clos_2127.
PATRIC20869616. VBIAlkOre124042_2234.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBCLSK976914.

Enzyme and pathway databases

BioCycAORE350688:GHBG-2188-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_ALKOO
AccessionPrimary (citable) accession number: A8MIN1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways