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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43S-adenosyl-L-methionineUniRule annotation1
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei95S-adenosyl-L-methionineUniRule annotation1
Active sitei117By similarity1
Binding sitei117S-adenosyl-L-methionineUniRule annotation1
Binding sitei121SubstrateUniRule annotation1
Binding sitei153SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Clos_2120
OrganismiAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Taxonomic identifieri350688 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
Proteomesi
  • UP000000269 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000643861 – 211tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST211

Interactioni

Protein-protein interaction databases

STRINGi350688.Clos_2120.

Structurei

3D structure databases

ProteinModelPortaliA8MIM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 193Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8MIM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVRNISNAK ELLKEYKEYV AAPQLFKEQW HTYFENQNPI HIEIGMGKGQ
60 70 80 90 100
FIRTLAKLNP NINYIGLEKV EELVLKSVRS IEAEEICNIS LINWNAVKLE
110 120 130 140 150
EILGEGEIER IYLNFSDPWP KARHDKRRLT HHGFLDIYQK LLKDHGEIHL
160 170 180 190 200
KTDSKALFEF SLEQFTQKNF TLVEVIEDLH GSTDKEIAKT EYEEKFIAEG
210
KPIYKCIARI E
Length:211
Mass (Da):24,803
Last modified:December 4, 2007 - v1
Checksum:i186984AB6B681DD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000853 Genomic DNA. Translation: ABW19656.1.
RefSeqiWP_012159965.1. NC_009922.1.

Genome annotation databases

EnsemblBacteriaiABW19656; ABW19656; Clos_2120.
KEGGiaoe:Clos_2120.
PATRICi20869602. VBIAlkOre124042_2227.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000853 Genomic DNA. Translation: ABW19656.1.
RefSeqiWP_012159965.1. NC_009922.1.

3D structure databases

ProteinModelPortaliA8MIM4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350688.Clos_2120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW19656; ABW19656; Clos_2120.
KEGGiaoe:Clos_2120.
PATRICi20869602. VBIAlkOre124042_2227.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_ALKOO
AccessioniPrimary (citable) accession number: A8MIM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 4, 2007
Last modified: November 2, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.