A8MHN7 (NADE_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Clos_1779

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077530
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity
Sites
	Active site	33	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MHN7 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 1FF2AA192F7C1D7F	FASTA	250	27,596
	10 20 30 40 50 60 MTQNIQKNID QVVEWLREQV RNAGAKGLTV GISGGIDSAV VACLIKKAFP ENSLGVILPI 70 80 90 100 110 120 KSSTKDVEHG ILTAEACGIE YIEIDLGEEH NTVANKVVHQ LNNKNLFNTG MERAMDSNLR 130 140 150 160 170 180 ARLRMSTLYA VANNLNYLVV GTDNAAEIYT GYFTKYGDGG VDILPIASLK KYEVYEWAKV 190 200 210 220 230 240 LGVPKEVLEK EPSAGLWEGQ TDEGEMGTSY KYIDEFLEGK AIPEKDLTVI ERLHRNSAHK 250 RTMPPSPKIG

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW19319.1.
RefSeq	YP_001513315.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MHN7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MHN7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5678709.
GenomeReviews	Gene locus Clos_1779 in contig CP000853_GR.
KEGG	aoe:Clos_1779.
PATRIC	20868810. VBIAlkOre124042_1860.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	YDISARD.
ProtClustDB	CLSK932624.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_1779-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_ALKOO

Accession

Primary (citable) accession number: A8MHN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program