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A8MH77

- DAPF_ALKOO

UniProt

A8MH77 - DAPF_ALKOO

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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131SubstrateUniRule annotation
Binding sitei47 – 471SubstrateUniRule annotation
Binding sitei65 – 651SubstrateUniRule annotation
Active sitei74 – 741Proton donor/acceptorUniRule annotation
Sitei165 – 1651Important for catalytic activityUniRule annotation
Binding sitei196 – 1961SubstrateUniRule annotation
Sitei214 – 2141Important for catalytic activityUniRule annotation
Active sitei223 – 2231Proton donor/acceptorUniRule annotation

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciAORE350688:GHBG-1450-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:Clos_1420
OrganismiAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Taxonomic identifieri350688 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
ProteomesiUP000000269: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Diaminopimelate epimerasePRO_1000058536Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi74 ↔ 223

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi350688.Clos_1420.

Structurei

3D structure databases

ProteinModelPortaliA8MH77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni74 – 763Substrate bindingUniRule annotation
Regioni214 – 2152Substrate bindingUniRule annotation
Regioni224 – 2252Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220466.
KOiK01778.
OMAiPAENICS.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8MH77-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVQFKKMQG TGNDFIVVKY DDFPFEEKLS QLAEKICDRH FGIGADGLLI
60 70 80 90 100
VNPSSIADIR MDYYNSDGSI AAMCGNGIRC FSKFVFDEGF LRTKQFSVET
110 120 130 140 150
LDGIKEIAII EEKGTVKSVE VNMGQVTYDT EKIPVVSEDG YFINKKITVG
160 170 180 190 200
GQDFIITAVS MGVPHVIIFT EKLDLEQIKF FGPLIEKHAI FPKKTNVNFV
210 220 230 240 250
HRIDKDNIAV RTWERGAGYT LACGTGSTSA VAVANKLGLV NNNVNVEVEG
260 270 280
GNIKIKIKES GNLFMEGPAE NICSGRFFFN
Length:280
Mass (Da):30,983
Last modified:December 4, 2007 - v1
Checksum:iA250AE65EC4C2419
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000853 Genomic DNA. Translation: ABW18964.1.
RefSeqiYP_001512960.1. NC_009922.1.

Genome annotation databases

EnsemblBacteriaiABW18964; ABW18964; Clos_1420.
GeneIDi5676454.
KEGGiaoe:Clos_1420.
PATRICi20868065. VBIAlkOre124042_1489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000853 Genomic DNA. Translation: ABW18964.1 .
RefSeqi YP_001512960.1. NC_009922.1.

3D structure databases

ProteinModelPortali A8MH77.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 350688.Clos_1420.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABW18964 ; ABW18964 ; Clos_1420 .
GeneIDi 5676454.
KEGGi aoe:Clos_1420.
PATRICi 20868065. VBIAlkOre124042_1489.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220466.
KOi K01778.
OMAi PAENICS.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci AORE350688:GHBG-1450-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OhILAs.

Entry informationi

Entry nameiDAPF_ALKOO
AccessioniPrimary (citable) accession number: A8MH77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3