Reviewed, UniProtKB/Swiss-Prot A8MH34 (MURD_ALKOO)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	Clos_1377

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000061450
Regions
	Nucleotide binding	117 – 123	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8MH34-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: F3B14E2E42650250	FASTA	454	50,251
	10 20 30 40 50 60 MNLKEKNVLV IGFAVTGIPL VKVLCQLGAN VIVNDSKKEE ALKESMKLLS DHPIQYILGK 70 80 90 100 110 120 HPEIEELSAP LDLVVVSPGV PLDIPFIENL RARGIEIIGE IELAYRLAQG HIVAITGTNG 130 140 150 160 170 180 KTTTTSLVGE IFKNAGRRTH VVGNIGVAFI SKALETTADD VIVIEASSFQ LESIVDFRPQ 190 200 210 220 230 240 VGAILNLTPD HLNRHKTMEN YQKAKFNIFK NQGAEDIAVI NYDDVKLREE SKQIYAKKVY 250 260 270 280 290 300 FSRRTLLEEG VFVEDGKIVA LKDGHKKEII SKDDIFIPGN HNLENALAAT AMTLSLGVDI 310 320 330 340 350 360 DVIQHTLKTF KGVEHRTEIV DVIHGVRFIN DSKGTNPDAS IKGIEGINTP ILLIAGGYDK 370 380 390 400 410 420 GSDFDEFINA FDGKVKHMFV YGETANTLME TAKKLNFIDV TKVQNLDEAV KKAYDIAIQG 430 440 450 DTVLLSPACA SWDMYENFEM RGKHFKEIVA NLRR

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000853 Genomic DNA. Translation: ABW18921.1.
RefSeq	YP_001512917.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5678739.
GenomeReviews	Gene locus Clos_1377 in contig CP000853_GR.
KEGG	aoe:Clos_1377.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SEDHLDR.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_ALKOO

Accession

Primary (citable) accession number: A8MH34

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)