A8MGI3 (SYA_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Clos_1666

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 880	880	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347486
Sites
	Metal binding	566	1	Zinc Potential
	Metal binding	570	1	Zinc Potential
	Metal binding	668	1	Zinc Potential
	Metal binding	672	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A8MGI3 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 7C060D18BD224C8D	FASTA	880	98,437
	10 20 30 40 50 60 MKKLGVNEIR KEFLEFFRSK EHIIHPSAPL VPQKDKSLLL INSGMAPLKP YFAGLEEPPG 70 80 90 100 110 120 KRMATCQKCI RTGDIENVGR TARHATFFEM LGNFSFGDYF KKESLTWGWE FVTKNLELPV 130 140 150 160 170 180 DKLWATVYVD DDEAFEIWNK QIGLPEERIV RLGKADNFWE IGLGPCGPCS EIYFDRGEDY 190 200 210 220 230 240 GCGCEECKPG CECDRYVEFW NHVFTQFDRD EEGNYHPLPN PNIDTGMGLE RMACIMQGVD 250 260 270 280 290 300 SIFDIDTMQD ILNSVCKITS SEYKKDERTD VSIRIITDHV RSISLMIGDG ILPSNEGRGY 310 320 330 340 350 360 VLRRLLRRAA RHGKLLGVNR AFLYELVDRV ADNYGETYVE LVDNKDYIKR VIKVEEERFM 370 380 390 400 410 420 ETIDQGMDIL NQYIEELVTL KETVLSGVNA FKLYDTYGFP FDLTKEILEE KGLSLDETGF 430 440 450 460 470 480 ESEMEKQRQR ARDARIGADT EGWKEDIFSG LDKEIQTAFK GYTNFEVEGK VLAIVSNDTV 490 500 510 520 530 540 VEQCDKGKEA TVILDETAFY GEGGGQVGDI GTLYNEAVRL SVLDTKKGPH NQVHHVVRVE 550 560 570 580 590 600 EGTIKIGDEV KAKVDMVTRM STARNHTATH LLHKALRDIV GEHVHQAGSL VTPDRLRFDF 610 620 630 640 650 660 THFEGLTKDQ ITQIEEKVNQ QILMALDVRT FETSIEDAKK IGAQALFGEK YGDVVRVVKV 670 680 690 700 710 720 GEYSTELCGG THVTNSGEIG MFIMLSEAGV AAGVRRIEAI TGMEAYKYVQ KNQKTIQEIA 730 740 750 760 770 780 DTLKTQVQNV VERVADLVHE TKEKDREINK LKSQLASNST GDILDKATVV GGINVVVEVL 790 800 810 820 830 840 ENQEMDDLRK IGDVLKEKIG SGVIVLGSSN QDKVNFVAMA TKDAVSKGVH AGNLVKEAAK 850 860 870 880 IAGGGGGGRP DMAQAGGKNP QKIQEALDTV KEILVNQLNQ

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW19206.1.
RefSeq	YP_001513202.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MGI3.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MGI3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5677913.
GenomeReviews	Gene locus Clos_1666 in contig CP000853_GR.
KEGG	aoe:Clos_1666.
PATRIC	20868578. VBIAlkOre124042_1745.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	MFTNSGM.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_1666-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ALKOO

Accession

Primary (citable) accession number: A8MGI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program