Reviewed, UniProtKB/Swiss-Prot A8MGI3 (SYA_ALKOO)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Clos_1666

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity HAMAP MF_00036.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 880	880	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347486

Sequences

	Sequence		Length	Mass (Da)
	A8MGI3-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 7C060D18BD224C8D	FASTA	880	98,437
	10 20 30 40 50 60 MKKLGVNEIR KEFLEFFRSK EHIIHPSAPL VPQKDKSLLL INSGMAPLKP YFAGLEEPPG 70 80 90 100 110 120 KRMATCQKCI RTGDIENVGR TARHATFFEM LGNFSFGDYF KKESLTWGWE FVTKNLELPV 130 140 150 160 170 180 DKLWATVYVD DDEAFEIWNK QIGLPEERIV RLGKADNFWE IGLGPCGPCS EIYFDRGEDY 190 200 210 220 230 240 GCGCEECKPG CECDRYVEFW NHVFTQFDRD EEGNYHPLPN PNIDTGMGLE RMACIMQGVD 250 260 270 280 290 300 SIFDIDTMQD ILNSVCKITS SEYKKDERTD VSIRIITDHV RSISLMIGDG ILPSNEGRGY 310 320 330 340 350 360 VLRRLLRRAA RHGKLLGVNR AFLYELVDRV ADNYGETYVE LVDNKDYIKR VIKVEEERFM 370 380 390 400 410 420 ETIDQGMDIL NQYIEELVTL KETVLSGVNA FKLYDTYGFP FDLTKEILEE KGLSLDETGF 430 440 450 460 470 480 ESEMEKQRQR ARDARIGADT EGWKEDIFSG LDKEIQTAFK GYTNFEVEGK VLAIVSNDTV 490 500 510 520 530 540 VEQCDKGKEA TVILDETAFY GEGGGQVGDI GTLYNEAVRL SVLDTKKGPH NQVHHVVRVE 550 560 570 580 590 600 EGTIKIGDEV KAKVDMVTRM STARNHTATH LLHKALRDIV GEHVHQAGSL VTPDRLRFDF 610 620 630 640 650 660 THFEGLTKDQ ITQIEEKVNQ QILMALDVRT FETSIEDAKK IGAQALFGEK YGDVVRVVKV 670 680 690 700 710 720 GEYSTELCGG THVTNSGEIG MFIMLSEAGV AAGVRRIEAI TGMEAYKYVQ KNQKTIQEIA 730 740 750 760 770 780 DTLKTQVQNV VERVADLVHE TKEKDREINK LKSQLASNST GDILDKATVV GGINVVVEVL 790 800 810 820 830 840 ENQEMDDLRK IGDVLKEKIG SGVIVLGSSN QDKVNFVAMA TKDAVSKGVH AGNLVKEAAK 850 860 870 880 IAGGGGGGRP DMAQAGGKNP QKIQEALDTV KEILVNQLNQ

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW19206.1.
RefSeq	YP_001513202.1.
3D structure databases
SMR	A8MGI3. Positions 4-464, 490-699.
ModBase	Search...
Genome annotation databases
GeneID	5677913.
GenomeReviews	Gene locus Clos_1666 in contig CP000853_GR.
KEGG	aoe:Clos_1666.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR009000. Transl_elong_init/rib_B-barrel. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ALKOO

Accession

Primary (citable) accession number: A8MGI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 4, 2007
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)