Reviewed, UniProtKB/Swiss-Prot A8MG12 (SYM1_ALKOO)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase 1
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase 1
      Short name=MetRS 1

Gene names

Name:	metG1
Ordered Locus Names:	Clos_1003

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	Methionyl-tRNA synthetase 1 HAMAP MF_00098		PRO_0000331778
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00098
	Motif	327 – 331	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	141	1	Zinc By similarity
	Metal binding	144	1	Zinc By similarity
	Metal binding	153	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Binding site	330	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MG12-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 7BBC960FEDA956DF	FASTA	540	62,728
	10 20 30 40 50 60 MNIFIGGAWP YANGSLHLGH VAALLPGDVM ARYFRAKGEN VLYVSGSDCH GTPISIRAKN 70 80 90 100 110 120 ENVSPQEIAE QYHKEFKYCF EELGFSYDYY SRTDDAYHKE EVQRIIKLLY ENEFIYEKEV 130 140 150 160 170 180 EQLYCADCNQ FLPDRFVEGI CPVCKNIARG DQCDVCSTIL DPLDLHHRKC KICGSEPEIK 190 200 210 220 230 240 NGNQLFFKLS KFQEMLQNHL EDSKKKWRVN ALNNTERYLK EGLQDRAISR DLNWGIEIPI 250 260 270 280 290 300 KGYEEKRVYV WIDAVLGYYT VSKKWGIERN RDWETFWSKE AIHYFIHGKD NIPFHSLIFP 310 320 330 340 350 360 ALLNGIGYKK MPDRIISSEY ITLEGKKIST SNNWAVWVPD MIERYNVDSI RYFLLANGPE 370 380 390 400 410 420 KRDADFSWRE FINSNNGELL GAYGNLVNRT FVFVKKYFNN TIPVGKLEEE IERATEKLYD 430 440 450 460 470 480 EVGKSIESGN LRLALEQIFQ FIRSINKYFD EETPWTTVNT NIDDCSNTIY NCLFSIINIA 490 500 510 520 530 540 NLLNPFLPSS SAKIKEWMGC KEASWNKLSL SSGIQLGDFN ILFERLDKKL AEEELAKLGR

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000853 Genomic DNA. Translation: ABW18550.1.
RefSeq	YP_001512546.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5675880.
GenomeReviews	Gene locus Clos_1003 in contig CP000853_GR.
KEGG	aoe:Clos_1003.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	DFSWREF.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM1_ALKOO

Accession

Primary (citable) accession number: A8MG12

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)