A8MG11 (GLPK_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Clos_1002

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Glycerol kinase HAMAP MF_00186		PRO_1000058443
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MG11 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 453A83872FCEE745	FASTA	504	56,167
	10 20 30 40 50 60 MKKYVMALDQ GTTSSRAILF DYEGKIVATS QKEFTQIYPK AGWVEHDPME IWGTQSGVAR 70 80 90 100 110 120 EVLERMAISP QDIAAIGITN QRETTIVWDK NTGKPVYNAI VWQCRRTAAI CDELKTQGMA 130 140 150 160 170 180 DYIRENTGLV LDAYFSGTKI KWILDHVEGA KEKAEKGELL FGTVDSWLIW NLTRGKVHVT 190 200 210 220 230 240 DYSNASRTML YNIKQLKWDD KILKALEIPK SMLPEVKESS AIYGYTDHQT FGGADIPIAG 250 260 270 280 290 300 AAGDQQAALF GQGCFKEGMG KNTYGTGCFM LMNTGDQFVQ SKNGLLTTLA WGIDGKVEYA 310 320 330 340 350 360 LEGSIFVAGA SVQWLRDELK IIRDAEDTEY LAKKVPNSNG VYVVPAFTGM GAPYWDMYAR 370 380 390 400 410 420 GAIVGLTRGA KAEHIIRATL ESIAYQTRDV LEAMEQDSGI QLKSLKVDGG AAMNNFLMQF 430 440 450 460 470 480 QADILSVPVD RPKITETTAL GAAYLAGLAV GFWKDKNEIE SKWSVDTVFE PGMDHEEKER 490 500 LYKGWKRAVN RALKWEEENE LDVK

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW18549.1.
RefSeq	YP_001512545.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MG11.
SMR	A8MG11. Positions 2-497.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MG11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5676744.
GenomeReviews	Gene locus Clos_1002 in contig CP000853_GR.
KEGG	aoe:Clos_1002.
PATRIC	20867165. VBIAlkOre124042_1043.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	SIAYQSN.
ProtClustDB	CLSK976238.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_1002-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_ALKOO

Accession

Primary (citable) accession number: A8MG11

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program