Reviewed, UniProtKB/Swiss-Prot A8MFY0 (TPIS_ALKOO)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	Clos_0971

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity. HAMAP MF_00147
Subcellular location	Cytoplasm Probable HAMAP MF_00147.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Triosephosphate isomerase HAMAP MF_00147		PRO_1000058107
Sites
	Active site	94	1	Electrophile By similarity
	Active site	166	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MFY0-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 4303995A9DE18DEC	FASTA	248	27,384
	10 20 30 40 50 60 MRKPIIAGNW KMNKVSKEAL DLVNQIKDEV HKTEVEVVVC CPFTVLSQVQ KALVGTNLKL 70 80 90 100 110 120 GAQNMHWEED GAYTGEISAN MLKDIGVEYV ILGHSERRQY FNETDETVNK KVKKAIKENL 130 140 150 160 170 180 KPIVCIGESL EEREANQTFD VIKKQLLGAF EGVPAEAMDN VVLAYEPIWA IGTGKTASSE 190 200 210 220 230 240 EAQTVIAYIR SLIEEKYGVD ISEEVRIQYG GSVKASNATE IMNETDIDGA LVGGASLKAE EFLGIINF

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW18518.1.
RefSeq	YP_001512514.1.
3D structure databases
SMR	A8MFY0. Positions 2-248.
ModBase	Search...
Genome annotation databases
GeneID	5677576.
GenomeReviews	Gene locus Clos_0971 in contig CP000853_GR.
KEGG	aoe:Clos_0971.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG708281.
OMA	DIRSVQT.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ALKOO

Accession

Primary (citable) accession number: A8MFY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	February 9, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)