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A8MFY0 (TPIS_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1
Alternative name(s):
Triose-phosphate isomerase
Gene names
Name:tpiA
Ordered Locus Names:Clos_0971
OrganismAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]
Taxonomic identifier350688 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B

Subunit structure

Homodimer By similarity. HAMAP MF_00147_B

Subcellular location

Cytoplasm By similarity HAMAP MF_00147_B.

Sequence similarities

Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
   Biological processGluconeogenesis
Glycolysis
Pentose shunt
   Cellular componentCytoplasm
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

glycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

pentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiontriose-phosphate isomerase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Triosephosphate isomerase HAMAP MF_00147_B
PRO_1000058107

Sites

Active site941Electrophile By similarity
Active site1661Proton acceptor By similarity
Binding site91Substrate By similarity
Binding site111Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A8MFY0 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 4303995A9DE18DEC

FASTA24827,384
        10         20         30         40         50         60 
MRKPIIAGNW KMNKVSKEAL DLVNQIKDEV HKTEVEVVVC CPFTVLSQVQ KALVGTNLKL 

        70         80         90        100        110        120 
GAQNMHWEED GAYTGEISAN MLKDIGVEYV ILGHSERRQY FNETDETVNK KVKKAIKENL 

       130        140        150        160        170        180 
KPIVCIGESL EEREANQTFD VIKKQLLGAF EGVPAEAMDN VVLAYEPIWA IGTGKTASSE 

       190        200        210        220        230        240 
EAQTVIAYIR SLIEEKYGVD ISEEVRIQYG GSVKASNATE IMNETDIDGA LVGGASLKAE 


EFLGIINF

« Hide

References

[1]"Complete genome of Alkaliphilus oremlandii OhILAs."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OhILAs.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000853 Genomic DNA. Translation: ABW18518.1.
RefSeqYP_001512514.1. NC_009922.1.

3D structure databases

ProteinModelPortalA8MFY0.
SMRA8MFY0. Positions 2-248.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8MFY0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5677576.
GenomeReviewsGene locus Clos_0971 in contig CP000853_GR.
KEGGaoe:Clos_0971.
PATRIC20867101. VBIAlkOre124042_1011.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG708281.
OMAVCVGETQ.
ProtClustDBPRK00042.

Enzyme and pathway databases

BioCycAORE350688:CLOS_0971-MONOMER.

Family and domain databases

HAMAPMF_00147_B. TIM_B.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01803.
PANTHERPTHR21139. Triophos_ismrse. 1 hit.
PfamPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMSSF51351. Triophos_ismrse. 1 hit.
TIGRFAMsTIGR00419. Tim. 1 hit.
PROSITEPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTPIS_ALKOO
AccessionPrimary (citable) accession number: A8MFY0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 4, 2007
Last modified: January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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