ID   A8MFW5_ALKOO            Unreviewed;       428 AA.
AC   A8MFW5;
DT   04-DEC-2007, integrated into UniProtKB/TrEMBL.
DT   04-DEC-2007, sequence version 1.
DT   27-MAR-2024, entry version 61.
DE   SubName: Full=Sarcosine reductase {ECO:0000313|EMBL:ABW18503.1};
DE            EC=1.21.4.3 {ECO:0000313|EMBL:ABW18503.1};
GN   OrderedLocusNames=Clos_0956 {ECO:0000313|EMBL:ABW18503.1};
OS   Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain
OS   OhILAs)).
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Alkaliphilus.
OX   NCBI_TaxID=350688 {ECO:0000313|EMBL:ABW18503.1, ECO:0000313|Proteomes:UP000000269};
RN   [1] {ECO:0000313|EMBL:ABW18503.1, ECO:0000313|Proteomes:UP000000269}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OhILAs {ECO:0000313|EMBL:ABW18503.1,
RC   ECO:0000313|Proteomes:UP000000269};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E.,
RA   Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.;
RT   "Complete genome of Alkaliphilus oremlandii OhILAs.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Proteomes:UP000000269}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OhILAs {ECO:0000313|Proteomes:UP000000269};
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E.,
RA   Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.;
RT   "Complete genome of Alkaliphilus oremlandii OhILAs.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000853; ABW18503.1; -; Genomic_DNA.
DR   RefSeq; WP_012158815.1; NC_009922.1.
DR   AlphaFoldDB; A8MFW5; -.
DR   STRING; 350688.Clos_0956; -.
DR   KEGG; aoe:Clos_0956; -.
DR   eggNOG; ENOG502Z7RC; Bacteria.
DR   HOGENOM; CLU_050376_0_0_9; -.
DR   OrthoDB; 5808629at2; -.
DR   Proteomes; UP000000269; Chromosome.
DR   GO; GO:0033794; F:sarcosine reductase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR016585; Gly/sarc/bet_Rdtase_B_asu/bsu.
DR   InterPro; IPR015417; Gly_reductase_pB_sua/b.
DR   Pfam; PF09338; Gly_reductase; 1.
DR   PIRSF; PIRSF011588; Gly_sarc_betain_red_a/b; 1.
PE   4: Predicted;
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000313|EMBL:ABW18503.1}; Pyruvate {ECO:0000256|PIRSR:PIRSR011588-51};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000269};
KW   Schiff base {ECO:0000256|PIRSR:PIRSR011588-50}.
FT   ACT_SITE        242
FT                   /note="Schiff-base intermediate with substrate; via pyruvic
FT                   acid"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR011588-50"
FT   MOD_RES         242
FT                   /note="Pyruvic acid (Cys)"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR011588-51"
SQ   SEQUENCE   428 AA;  46288 MW;  21CD570551ACBB83 CRC64;
     MRLELGNIFI QDVKFGEATK VENKILYINK DEMLKELGGD ERIKSIDIEL AHPGEEVRIT
     PVKDVIEPRV KVEGPGGIFP GVINKVDTVG SGRTHALKNV AVVTAGKIVG FQEGIIDMSG
     PGAEYTPFSG LHNIVVVAEP IDGLKQYDHE AAVRMIGFKA AIYLGEAARN VEPDEVKAYE
     TEPIFESANK YPDLPKVGYV YMLQTQGLLH DTYVYGVDAK KIVPTILYPT EIMDGAIVSG
     NCVSACDKNP TYVHLNNGII EDLYEKHGKE LNFVGVIITN ENVYLADKER SSNWTAKLCK
     FLGLDGVIVS QEGFGNPDTD LIMNCKKIEG EGIKTVIVTD EYAGRDGASQ SLADADVKAN
     AVVTNGNANE TIVLPPMKKI IGTTDYVDKI AGGFDGSLRA DGSIEVEIQA ITGATNETGF
     GRLTAKGF
//