A8MFK5 (AROK_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate kinase
Short name=SK
EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	Clos_1625

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm By similarity HAMAP MF_00109.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW shikimate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Shikimate kinase HAMAP MF_00109		PRO_1000094370
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
Sites
	Metal binding	16	1	Magnesium By similarity
	Binding site	34	1	Substrate By similarity
	Binding site	58	1	Substrate By similarity
	Binding site	80	1	Substrate; via amide nitrogen By similarity
	Binding site	119	1	ATP By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	156	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MFK5 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: B48EA0A2338AAA53	FASTA	169	19,292
	10 20 30 40 50 60 MVKNIVLVGF MAVGKTTLGK LLADQLDYNF IDLDLFIEKK ESMSISEIFR LKGEAYFRQK 70 80 90 100 110 120 ESEALDLLSD VEQSVIATGG GTVISEENRS KLKQIGRVIY LEAEPSWILT NIKRSAVIRP 130 140 150 160 LLVDERKSMD KIIEILENRR LYYEGTSEIK IPVSHRTLEE IIKDIVCNI

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW19168.1.
RefSeq	YP_001513164.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MFK5.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MFK5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5678198.
GenomeReviews	Gene locus Clos_1625 in contig CP000853_GR.
KEGG	aoe:Clos_1625.
PATRIC	20868478. VBIAlkOre124042_1695.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335815.
OMA	GEPYFRN.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_1625-MONOMER.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. [Tree]
InterPro	IPR000623. Shikimate_kinase. IPR023000. Shikimate_kinase_CS. [Graphical view]
KO	K00891.
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_ALKOO

Accession

Primary (citable) accession number: A8MFK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program