Reviewed, UniProtKB/Swiss-Prot A8MFI7 (DXS_ALKOO)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Clos_1607

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000059442

Sequences

	Sequence		Length	Mass (Da)
	A8MFI7-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 1F212F8F23B074B7	FASTA	624	69,328
	10 20 30 40 50 60 MYKYLMEINS IEDFKKLNSN EIKVLAEEIR HFLIESVSKT GGHLASNLGV VELTLALHQA 70 80 90 100 110 120 FNSPEDKIIW DVGHQAYVHK ILTGRRDQFS TLRQYKGLSG FPKRYESEHD QFDTGHSSTS 130 140 150 160 170 180 ISAAMGLATA RDLNKDKYKV IAVIGDGAMT GGMAFEALNH IGQSQKDIIV ILNDNEMSIS 190 200 210 220 230 240 PNVGGLSNYL NKIRTAPIYS KVKDDVEYLI SNIPAIGKSV MKTAEKAKDS IKYFFVPGVL 250 260 270 280 290 300 FEELGFTYIG PVDGHNYHSL YDVMNRTKNI KGPVLLHVMT TKGKGYTLAE KHPDKYHGVN 310 320 330 340 350 360 SFHIETGEPI SCNENLSYSE IAGKTLVECA AEDEKIVAIT AAMPSGTGLN NFAKKHPERF 370 380 390 400 410 420 FDVGIAEQHA ATFAAGLAAN GFKPFFAVYS TFFQRAYDQV IHDACIQDLP VTYLIDRAGL 430 440 450 460 470 480 VGNDGETHHG SLDISFLSCI PNLTFMAPKD GIELSEMVRF AAKHNGPVAI RYPRGHSNMD 490 500 510 520 530 540 SQENFSPLAL GKGEITYHSG NDVLILALGT FNKMGLEICK DLEENNIFST LMNPRFIKPM 550 560 570 580 590 600 DEELIVEMVQ KHKIIYTIED NSKIGGFGTL VQVLLNDNQI LKPVKVCALP DRFIEHGNVE 610 620 DLYEELGLTK KQIVDQIKKE FSDL

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW19150.1.
RefSeq	YP_001513146.1.
3D structure databases
SMR	A8MFI7. Positions 2-620.
ModBase	Search...
Genome annotation databases
GeneID	5678080.
GenomeReviews	Gene locus Clos_1607 in contig CP000853_GR.
KEGG	aoe:Clos_1607.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	QRFPDRY.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme_bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ALKOO

Accession

Primary (citable) accession number: A8MFI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)