Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A8MFB4

- PNP_ALKOO

UniProt

A8MFB4 - PNP_ALKOO

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi485 – 4851MagnesiumUniRule annotation
    Metal bindingi491 – 4911MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciAORE350688:GHBG-1564-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:Clos_1534
    OrganismiAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
    Taxonomic identifieri350688 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
    ProteomesiUP000000269: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 706706Polyribonucleotide nucleotidyltransferasePRO_0000329495Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi350688.Clos_1534.

    Structurei

    3D structure databases

    ProteinModelPortaliA8MFB4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini552 – 61160KHUniRule annotationAdd
    BLAST
    Domaini621 – 68969S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218327.
    KOiK00962.
    OMAiRASAENQ.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8MFB4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKVFKKEIA GRVLEVEVGK LAQLSNGSCL LKYGQTAVLV NACASKSPRE    50
    GIDFFPLSVD YEEKMYAAGK IPGGFIKREG RPSENAILTS RLIDRPIRPL 100
    FPDGYRNDVQ IIATVLSVDI DCTPDIVAML GSSIALSISD IPFQGPTASV 150
    HVGLVDGKLL INPTSDEREH SSLDLVVSGT KDAVMMIEAG ANEVTEAQML 200
    EAILFAHEEI KNIISFIEEI AAEVGKEKQE VVTLEINEDF ETEVRAYAKT 250
    RISEAIRTVE KLERNAKIEL ANEETINYFY EKYQEELNLK QIHTILDSIL 300
    KEETRNLILY DKKRPDDRQH DEIRPISCEV DLLPKTHGSG LFTRGQTQVL 350
    SIVTLGAIGD VQILDGLGKE ESKRYMHHYN FPPYSVGETK MLRSPGRREI 400
    GHGALAERAL EPMIPSQDDF PYAIRVVSEV LASNGSSSQA SVCGSTLSLL 450
    DAGVPLKDMV AGIAMGLVKE GDAVAILSDI QGMEDHLGDM DFKVAGTAKG 500
    ITAIQMDIKI KGIDEPILKD ALEQARVGRL YILDKMKEAI SSPRPELSPY 550
    APRMLKMKIH PDKIREVIGS GGKTINKIIE DTGVKIDIEN DGTIFIAAQT 600
    QEAGELALSI INNIVREPEI GDIFKGKVIK LMNFGAFVEI LPGKEGLVHI 650
    SNLAHERVNK VEDVLAVGDE IDVKVIEIDQ QGKVSLSRKA LLPKDNDKKQ 700
    DDPQKA 706
    Length:706
    Mass (Da):77,654
    Last modified:December 4, 2007 - v1
    Checksum:i38174443246F1D5D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000853 Genomic DNA. Translation: ABW19077.1.
    RefSeqiYP_001513073.1. NC_009922.1.

    Genome annotation databases

    EnsemblBacteriaiABW19077; ABW19077; Clos_1534.
    GeneIDi5677572.
    KEGGiaoe:Clos_1534.
    PATRICi20868296. VBIAlkOre124042_1604.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000853 Genomic DNA. Translation: ABW19077.1 .
    RefSeqi YP_001513073.1. NC_009922.1.

    3D structure databases

    ProteinModelPortali A8MFB4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 350688.Clos_1534.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABW19077 ; ABW19077 ; Clos_1534 .
    GeneIDi 5677572.
    KEGGi aoe:Clos_1534.
    PATRICi 20868296. VBIAlkOre124042_1604.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218327.
    KOi K00962.
    OMAi RASAENQ.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci AORE350688:GHBG-1564-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: OhILAs.

    Entry informationi

    Entry nameiPNP_ALKOO
    AccessioniPrimary (citable) accession number: A8MFB4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3