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A8MF40 (DAPA_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:Clos_1163
OrganismAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]
Taxonomic identifier350688 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Dihydrodipicolinate synthase HAMAP MF_00418
PRO_1000080520

Regions

Region47 – 482Pyruvate binding By similarity

Sites

Active site1601Schiff-base intermediate with substrate By similarity
Binding site1051Pyruvate By similarity
Site1321Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
A8MF40 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 62908D3859BD0E33

FASTA29031,872
        10         20         30         40         50         60 
MFKGAGVAIV TPFKEGKIDF AAFEKLIDFH LENNTQALIV LGTTGEASTQ TMEEREALIR 

        70         80         90        100        110        120 
LAVQRVEGRI PVIVGTGSNC TDTALQYTRQ AEDLGADGIL VVTPYYNKCT QKGLIEHFTQ 

       130        140        150        160        170        180 
IANATKLPVI LYNVPSRTGV NILPETVATM SKVENVIGIK EAGGNTAQVL EIKRLVPEDF 

       190        200        210        220        230        240 
KIYSGNDDQI IPIYACGGHG VISVASNVIP KEIQEMCAAF MEGNVEKALE IQLLYKKFID 

       250        260        270        280        290 
LLFCEVNPIP VKAAVSALGY IENELRLPLT PMEEVNRKKL MDEMKKQNII

« Hide

References

[1]"Complete genome of Alkaliphilus oremlandii OhILAs."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OhILAs.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000853 Genomic DNA. Translation: ABW18709.1.
RefSeqYP_001512705.1. NC_009922.1.

3D structure databases

ProteinModelPortalA8MF40.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8MF40.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5676461.
GenomeReviewsGene locus Clos_1163 in contig CP000853_GR.
KEGGaoe:Clos_1163.
PATRIC20867515. VBIAlkOre124042_1216.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG358848.
OMATIHFTHI.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycAORE350688:CLOS_1163-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_ALKOO
AccessionPrimary (citable) accession number: A8MF40
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 4, 2007
Last modified: January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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