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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551SubstrateUniRule annotation
Binding sitei190 – 1901SubstrateUniRule annotation
Metal bindingi196 – 1961ManganeseUniRule annotation
Binding sitei196 – 1961ATPUniRule annotation
Binding sitei196 – 1961SubstrateUniRule annotation
Metal bindingi215 – 2151Manganese; via tele nitrogenUniRule annotation
Binding sitei215 – 2151ATPUniRule annotation
Metal bindingi252 – 2521ManganeseUniRule annotation
Binding sitei280 – 2801ATPUniRule annotation
Binding sitei317 – 3171ATPUniRule annotation
Binding sitei317 – 3171SubstrateUniRule annotation
Binding sitei442 – 4421ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi231 – 2399ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAORE350688:GHBG-908-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:Clos_0884
OrganismiAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs))
Taxonomic identifieri350688 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus
Proteomesi
  • UP000000269 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 526526Phosphoenolpyruvate carboxykinase [ATP]PRO_1000072372Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi350688.Clos_0884.

Structurei

3D structure databases

ProteinModelPortaliA8MEV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG6DG2RK.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8MEV2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNVKELIDS GILKTNKIHY NLPVEELIKI AVEKEGGVIA KNGALCINTG
60 70 80 90 100
KYTGRSPEDR FIVDEPSVHD HINWNKGNKG ISAQVFDNLF NKAMAHVKDK
110 120 130 140 150
ELFVFEGFVG SDLDYRMPIR VINEFAYQNL FASQMFIKPK AGERENFDPE
160 170 180 190 200
FTVLALPDLK ADPAVDGTNS EVFIILNFAQ KIVLIGGTRY SGEIKKSIFT
210 220 230 240 250
VMNYLLPFKD VLPMHCSANI GEDGNVALFF GLSGTGKTTL SADNNRKLIG
260 270 280 290 300
DDEHGWTANG VFNFEGGCYA KCINLTEEQE PQIWNAIKHG AILENIVIDE
310 320 330 340 350
NTGHIDYDDD RYTENTRAAY PVEYIDDAVL EGKGGIPNTI IFLTADATGV
360 370 380 390 400
LPPIAKLTKE QAMYHFMSGY TSKLAGTERG IVEPTATFST CFGGPFMLLK
410 420 430 440 450
PQTYAKLLGE KIEKYNTRVF LVNTGWTGGP YGIGSRMKLK YTRSMVRAAI
460 470 480 490 500
KGELDQVEYI KDPIFNLSIP TTCPEVPVEV LEPSKTWDNQ DEYDQKANEL
510 520
AKSFVENFKK FDDVSDDIKN VNPKVQ
Length:526
Mass (Da):58,663
Last modified:December 4, 2007 - v1
Checksum:i195DF3B2804957A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000853 Genomic DNA. Translation: ABW18431.1.
RefSeqiWP_012158743.1. NC_009922.1.

Genome annotation databases

EnsemblBacteriaiABW18431; ABW18431; Clos_0884.
KEGGiaoe:Clos_0884.
PATRICi20866923. VBIAlkOre124042_0922.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000853 Genomic DNA. Translation: ABW18431.1.
RefSeqiWP_012158743.1. NC_009922.1.

3D structure databases

ProteinModelPortaliA8MEV2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350688.Clos_0884.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW18431; ABW18431; Clos_0884.
KEGGiaoe:Clos_0884.
PATRICi20866923. VBIAlkOre124042_0922.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiEOG6DG2RK.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciAORE350688:GHBG-908-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OhILAs.

Entry informationi

Entry nameiPCKA_ALKOO
AccessioniPrimary (citable) accession number: A8MEV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 4, 2007
Last modified: December 9, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.