A8MEG7 (GCSPB_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glycine dehydrogenase [decarboxylating] subunit 2
EC=1.4.4.2

Alternative name(s):
Glycine cleavage system P-protein subunit 2
Glycine decarboxylase subunit 2

Gene names

Name:	gcvPB
Ordered Locus Names:	Clos_0068

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus › Alkaliphilus oremlandii

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP-Rule MF_00713
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP-Rule MF_00713
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits By similarity.
Sequence similarities	Belongs to the GcvP family. C-terminal subunit subfamily.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Molecular_function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Probable glycine dehydrogenase [decarboxylating] subunit 2 HAMAP-Rule MF_00713		PRO_1000062080
Amino acid modifications
	Modified residue	273	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8MEG7 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: F4CF441B7DFB1782	FASTA	486	54,111
	10 20 30 40 50 60 MKSDYNKLIF EISKEGRKAY SLPKCDVEEV NLESLIPKEF LSDKELDLPE VSEVDVIRHY 70 80 90 100 110 120 TQLSNKNYGV DTGFYPLGSC TMKYNPKLNE DMAVLPGFAN IHPYQPEETV QGALELMYKL 130 140 150 160 170 180 DKMLAEVAGM ERMTLQPAAG AHGELVGLMV IKAYHKKRGD LKRTKIIIPD SAHGTNPASA 190 200 210 220 230 240 AVAGFDVVEI KSNPDGSVNI DSLKSALSDE IAGLMLTNPS TLGLFETNIK QIADLVHDAG 250 260 270 280 290 300 GLLYYDGANM NAIMGVTRPG DMGFDVIHYN IHKTFSTPHG GGGPGSGPVG VRKDLVEFLP 310 320 330 340 350 360 SPVIEKKGEE YVLDYDRPYS IGKIKSFYGH FGILVRAYTY ILSYGPALRE VSEKAVLNAN 370 380 390 400 410 420 YMMHKLKEKY YLPIEQVCKH EFVLGGLNED ILGVSTLDIA KRLLDYGYHP PTIYFPLIVN 430 440 450 460 470 480 EAMMIEPTET ESVETLDQFI DALNKIADEA KETPELLKNA PHHTHVRRID EAKAARNLIV KWEREQ

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW17638.1.
RefSeq	YP_001511634.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MEG7.
SMR	A8MEG7. Positions 7-483.
ModBase	Search...
Protein-protein interaction databases
STRING	350688.Clos_0068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABW17638; ABW17638; Clos_0068.
GeneID	5675981.
KEGG	aoe:Clos_0068.
PATRIC	20865204. VBIAlkOre124042_0073.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1003.
HOGENOM	HOG000239368.
KO	K00283.
OMA	RHYTRLS.
ProtClustDB	PRK04366.
Enzyme and pathway databases
BioCyc	AORE350688:GHBG-183-MONOMER.
Family and domain databases
Gene3D	3.40.640.10. 1 hit.
HAMAP	MF_00713. GcvPB.
InterPro	IPR020580. GDC-P_N. IPR020581. GDC_P. IPR023012. GDC_P_su2. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11773. PTHR11773. 1 hit.
Pfam	PF02347. GDC-P. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCSPB_ALKOO

Accession

Primary (citable) accession number: A8MEG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program