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A8MEG7 (GCSPB_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable glycine dehydrogenase (decarboxylating) subunit 2

EC=1.4.4.2
Alternative name(s):
Glycine cleavage system P-protein subunit 2
Glycine decarboxylase subunit 2
Glycine dehydrogenase (aminomethyl-transferring) subunit 2
Gene names
Name:gcvPB
Ordered Locus Names:Clos_0068
OrganismAlkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]
Taxonomic identifier350688 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeAlkaliphilus

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP-Rule MF_00713

Catalytic activity

Glycine + [glycine-cleavage complex H protein]-N(6)-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N(6)-dihydrolipoyl-L-lysine + CO2. HAMAP-Rule MF_00713

Cofactor

Pyridoxal phosphate By similarity. HAMAP-Rule MF_00713

Subunit structure

The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is a heterodimer of two subunits By similarity.

Sequence similarities

Belongs to the GcvP family. C-terminal subunit subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Probable glycine dehydrogenase (decarboxylating) subunit 2 HAMAP-Rule MF_00713
PRO_1000062080

Amino acid modifications

Modified residue2731N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
A8MEG7 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: F4CF441B7DFB1782

FASTA48654,111
        10         20         30         40         50         60 
MKSDYNKLIF EISKEGRKAY SLPKCDVEEV NLESLIPKEF LSDKELDLPE VSEVDVIRHY 

        70         80         90        100        110        120 
TQLSNKNYGV DTGFYPLGSC TMKYNPKLNE DMAVLPGFAN IHPYQPEETV QGALELMYKL 

       130        140        150        160        170        180 
DKMLAEVAGM ERMTLQPAAG AHGELVGLMV IKAYHKKRGD LKRTKIIIPD SAHGTNPASA 

       190        200        210        220        230        240 
AVAGFDVVEI KSNPDGSVNI DSLKSALSDE IAGLMLTNPS TLGLFETNIK QIADLVHDAG 

       250        260        270        280        290        300 
GLLYYDGANM NAIMGVTRPG DMGFDVIHYN IHKTFSTPHG GGGPGSGPVG VRKDLVEFLP 

       310        320        330        340        350        360 
SPVIEKKGEE YVLDYDRPYS IGKIKSFYGH FGILVRAYTY ILSYGPALRE VSEKAVLNAN 

       370        380        390        400        410        420 
YMMHKLKEKY YLPIEQVCKH EFVLGGLNED ILGVSTLDIA KRLLDYGYHP PTIYFPLIVN 

       430        440        450        460        470        480 
EAMMIEPTET ESVETLDQFI DALNKIADEA KETPELLKNA PHHTHVRRID EAKAARNLIV 


KWEREQ 

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References

[1]"Complete genome of Alkaliphilus oremlandii OhILAs."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OhILAs.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000853 Genomic DNA. Translation: ABW17638.1.
RefSeqYP_001511634.1. NC_009922.1.

3D structure databases

ProteinModelPortalA8MEG7.
SMRA8MEG7. Positions 7-483.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING350688.Clos_0068.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABW17638; ABW17638; Clos_0068.
GeneID5675981.
KEGGaoe:Clos_0068.
PATRIC20865204. VBIAlkOre124042_0073.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1003.
HOGENOMHOG000239368.
KOK00283.
OMAWTGLMMI.
OrthoDBEOG6HMXDX.

Enzyme and pathway databases

BioCycAORE350688:GHBG-83-MONOMER.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
HAMAPMF_00713. GcvPB.
InterProIPR020580. GDC-P_N.
IPR020581. GDC_P.
IPR023012. GDC_P_su2.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11773. PTHR11773. 1 hit.
PfamPF02347. GDC-P. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGCSPB_ALKOO
AccessionPrimary (citable) accession number: A8MEG7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 4, 2007
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families