A8MEG4 (GCST_ALKOO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	Clos_0065

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Aminomethyltransferase HAMAP MF_00259		PRO_1000059078

Sequences

	Sequence		Length	Mass (Da)
	A8MEG4 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: E75FAE97D052FAE6	FASTA	368	41,165
	10 20 30 40 50 60 MTELKRTPLF DAHNRYGGKL VDFAGWEMSV QFEGLTAEHE AVRNAAGIFD VSHMGEIEVR 70 80 90 100 110 120 GKDAEAFVQY LVTNDVAALE DNQIVYTFMC YPDGGIVDDL LVYKFNKEYY YLVVNASNSD 130 140 150 160 170 180 KDFAWMNENK GAYDVEIINI SDSVSQVAVQ GPKAEEIVQE LTDTDLSEIP FFYFKNDVVI 190 200 210 220 230 240 NGANCLISRT GYTGEDGFEI YVDNDKVDAL WDKIIEVGKD RGLKPAGLGA RDTLRFEATL 250 260 270 280 290 300 PLYGHEIDKD ISPLEAGLGF FVKLNKENFI GKDALVRQKE EGLKRKVVGF EMKENGIPRQ 310 320 330 340 350 360 GYEVKVGDKV IGVVTTGYNS PTLKKNIGYA LIDAEYAALG TPIDIQVRKK TLKAEVVSKK FYTRSTKK

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OhILAs.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000853 Genomic DNA. Translation: ABW17635.1.
RefSeq	YP_001511631.1. NC_009922.1.
3D structure databases
ProteinModelPortal	A8MEG4.
SMR	A8MEG4. Positions 4-361.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MEG4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5675978.
GenomeReviews	Gene locus Clos_0065 in contig CP000853_GR.
KEGG	aoe:Clos_0065.
PATRIC	20865198. VBIAlkOre124042_0070.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299834.
OMA	VINASNK.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	AORE350688:CLOS_0065-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_ALKOO

Accession

Primary (citable) accession number: A8MEG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program