Reviewed, UniProtKB/Swiss-Prot A8MEE1 (AROE_ALKOO)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	Clos_0042

Organism

Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain OhILAs)) [Complete proteome] [HAMAP]

Taxonomic identifier

350688 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Alkaliphilus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000058659
Regions
	Nucleotide binding	133 – 137	5	NADP By similarity
Sites
	Active site	73	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A8MEE1-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: B3141D5F0A3A85C9	FASTA	286	31,523
	10 20 30 40 50 60 MSYVITDETK LTGIIGRPIK QSFSPKIHNG AFQYLNLNYI YIPFEVVQQD LSKTVEAMKV 70 80 90 100 110 120 LKFRGFNVTM PHKREVMNYL DEVSENAKII GAVNTVVNQN GKLVGHNTDG KGYVQSLEDE 130 140 150 160 170 180 GIYVKEKTFV IAGAGGAARS VAVQLALDGA KKITILNRTI DKAHDIAQLI GMSIPNVHIE 190 200 210 220 230 240 INCLENKTLA KAVEEADVLI NTTSLGMYSM EEESIIGEEK VLPSNLVVSD LIYNPAKTKL 250 260 270 280 LQQAESRGCK TINGLGMLIG QAAIAFELWT EVAMPIDYIK KTLFTR

References

[1]

"Complete genome of Alkaliphilus oremlandii OhILAs." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E., Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000853 Genomic DNA. Translation: ABW17612.1.
RefSeq	YP_001511608.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5677725.
GenomeReviews	Gene locus Clos_0042 in contig CP000853_GR.
KEGG	aoe:Clos_0042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8MEE1. MEARIDG.
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_ALKOO

Accession

Primary (citable) accession number: A8MEE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 4, 2007
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)