Reviewed, UniProtKB/Swiss-Prot A8ME45 (RIFK_CALMQ)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	Cmaq_1224

Organism

Caldivirga maquilingensis (strain DSMZ 13496 / IC-167) [Complete proteome] [HAMAP]

Taxonomic identifier

397948 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Caldivirga

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Riboflavin kinase HAMAP MF_01285		PRO_0000322081
Regions
	Nucleotide binding	111 – 116	6	CDP By similarity
	Nucleotide binding	210 – 213	4	CDP By similarity
	Region	1 – 101	101	H-T-H motif-like HAMAP MF_01285
	Region	102 – 230	129	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	140	1	Magnesium By similarity
	Metal binding	142	1	Magnesium By similarity
	Binding site	197	1	FMN By similarity
	Binding site	205	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ME45-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: F07DCE6137600F12	FASTA	230	25,887
	10 20 30 40 50 60 MDKQVNVVNY RELKKIPYIL LLLQNGVNDH DFTRISVSDL SKQMGTTPQN ISKVLRRLER 70 80 90 100 110 120 EGYIVRSSVK GEVSVMLSEK GSALLRNLMD LMENLLGKNI TIVLRGIVVT GFGEGSYYIS 130 140 150 160 170 180 LEGYRRQFIS KLGFDPYPGT LNVKLLDQYM KYRLYLERVP GVRIEGFSNG SRTYGGVKAF 190 200 210 220 230 KCTISDIPCG VLLIERTSHG PEVIEIVAPV KLRDRLGLKD GDDVTINILL

References

[1]

"Complete sequence of Caldivirga maquilingensis IC-167." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Ivanova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M., Saltikov C., House C.H., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000852 Genomic DNA. Translation: ABW02051.1.
RefSeq	YP_001541041.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5710113.
GenomeReviews	Gene locus Cmaq_1224 in contig CP000852_GR.
KEGG	cma:Cmaq_1224.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GAYYVRQ.
Family and domain databases
HAMAP	MF_01285. Fused. [Tree]
InterPro	IPR000835. HTH_MarR. IPR002834. Riboflavin_kinase_arc. IPR000485. Tscrpt_reg_AsnC-typ_HTH_dom. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. PF01047. MarR. 1 hit. [Graphical view]
PRINTS	PR00033. HTHASNC.
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RIFK_CALMQ

Accession

Primary (citable) accession number: A8ME45

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)