A8MCK2 (PURL_CALMQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Cmaq_0668

Organism

Caldivirga maquilingensis (strain ATCC 700844 / DSMZ 13496 / JCM 10307 / IC-167) [Complete proteome] [HAMAP]

Taxonomic identifier

397948 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Caldivirga › Caldivirga maquilingensis

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_1000080551
Regions
	Nucleotide binding	90 – 101	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8MCK2 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 031A5827388C6011	FASTA	724	77,413
	10 20 30 40 50 60 MGLTSDEAKI IINTLGRNPT EAEWLIFEAE WSEHCSYKSS RAWIRLLPSK SPLVIRGSGL 70 80 90 100 110 120 DAPIIRINNI AVTFKIESHN HPSAVDPYDG AATGVGGIVR DILTTGLRPI ALLDNLHLGS 130 140 150 160 170 180 LDNQRSLWLS RNIIKGISDY GNRIGVPVVG GETWFDESFN NNPIVLVTCI GAGDFKRVIW 190 200 210 220 230 240 GEGKVGDLVL VVGNDTGRDG MLGSSFASRE LSSSDDIGAV QVGNPLLEKL LIDALMELGE 250 260 270 280 290 300 RGLVKAIKDV GGGGLATALS ELAHQLGLGI EVDLTNIRLR DELKPEEILV SESQERMIIV 310 320 330 340 350 360 VDPSRLSYVE AVLRKYEVGF DLIGKLTNDG KFTAYYKGIK LIDLPLDLIT NPPEPIRKYT 370 380 390 400 410 420 EPVYLMRLRR IPPLPSVKFN EALIKVASSP NLASKEVIYT QYDYEVGVRT VIKPGRAGAT 430 440 450 460 470 480 VLRLLEEDGG DGKLGIAVKA DSNPRYSYLN PFTGAANSLA KAYRNVASVG AKPIAAVDSI 490 500 510 520 530 540 NVGNPEKPDK YWYFVKTVEG LTWMGNALGI PFVGGKVSFY NEDSVTGASI KPVVAVAVLG 550 560 570 580 590 600 VVNDYAKAIE GGLTGEGWLV IIGDTGPELG GSEFLHSVHG LVAGEPPEPK PLSEVKNANL 610 620 630 640 650 660 VMQLINNGLA KAVMDVGVGG LAAALIKMSI IGGVGFTVDL SKAPLTQGLN DPVTVAFSET 670 680 690 700 710 720 NARYIIETSN LKETVRIIEA NGVPYGVLGE SGGGIVQFKW GGLELASLSV DDLVSINDSL RGVI

References

[1]

"Complete sequence of Caldivirga maquilingensis IC-167." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Ivanova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M., Saltikov C., House C.H., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700844 / DSMZ 13496 / JCM 10307 / IC-167.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000852 Genomic DNA. Translation: ABW01508.1.
RefSeq	YP_001540498.1. NC_009954.1.
3D structure databases
ProteinModelPortal	A8MCK2.
ModBase	Search...
Protein-protein interaction databases
STRING	397948.Cmaq_0668.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABW01508; ABW01508; Cmaq_0668.
GeneID	5710326.
KEGG	cma:Cmaq_0668.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	CMAQ397948:GH9M-797-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_CALMQ

Accession

Primary (citable) accession number: A8MCK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program