A8MC04 (SYP_CALMQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Cmaq_1972

Organism

Caldivirga maquilingensis (strain ATCC 700844 / DSMZ 13496 / JCM 10307 / IC-167) [Complete proteome] [HAMAP]

Taxonomic identifier

397948 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Caldivirga

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Proline--tRNA ligase HAMAP MF_01571		PRO_1000215547

Sequences

	Sequence		Length	Mass (Da)
	A8MC04 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 466D63DF3D7D93A6	FASTA	481	55,799
	10 20 30 40 50 60 MVRGPQGRPR SRWVSFIEWF NKVIMDAEVY DYRYPVKGAY IWRPYGVAIR RNVEALIRRL 70 80 90 100 110 120 HDETGHQEVL FPVFIPYEFF SKESEHIRGF ESEVFWVSKG TGGEERLVLR PTSETAMMPM 130 140 150 160 170 180 FKLWIRDHTD LPLRVYQIVS VFRAETKMTH PMIRLREISM FKEAHTAHAD RDDAERQVKE 190 200 210 220 230 240 AVGIYRRIMD ELCIPYLISR RPDWDKFAGA VYTIAFDTIM PDGRTMQIGT VHYLGENFSR 250 260 270 280 290 300 VFDVKYLGKD GQMHYIHTTS YGISERIIAS MIAVNGDDRG LLLPPRYAPI QVVVIPIMYG 310 320 330 340 350 360 EDQSVLNYAK GVSGELLNAG VRVHVDDRRD KTPGWKYYHW ELKGVPIRLE VGPSDVKDNA 370 380 390 400 410 420 VTLTRRDTFE KYAVERSNVV DAVRELMKAI EDNMRKSTWE WLRSHVRRSS NVSEAKALLN 430 440 450 460 470 480 EGGVVEVPWS GDDECGRRIM ELTESDALGI PLDTDETPSD LRDAACSEKK AEYWLRLSRR Y

References

[1]

"Complete sequence of Caldivirga maquilingensis IC-167." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Ivanova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M., Saltikov C., House C.H., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700844 / DSMZ 13496 / JCM 10307 / IC-167.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000852 Genomic DNA. Translation: ABW02788.1.
RefSeq	YP_001541778.1. NC_009954.1.
3D structure databases
ProteinModelPortal	A8MC04.
ModBase	Search...
Protein-protein interaction databases
STRING	A8MC04.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5708446.
GenomeReviews	Gene locus Cmaq_1972 in contig CP000852_GR.
KEGG	cma:Cmaq_1972.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG334108.
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	CMAQ397948:CMAQ_1972-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_CALMQ

Accession

Primary (citable) accession number: A8MC04

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program